scholarly journals Visual detection of Fusarium proliferatum based on asymmetric recombinase polymerase amplification and hemin/G-quadruplex DNAzyme

RSC Advances ◽  
2019 ◽  
Vol 9 (64) ◽  
pp. 37144-37147 ◽  
Author(s):  
Ying Wang ◽  
Xiangdong Li ◽  
Dongmei Xi ◽  
Xiaoqiang Wang
Keyword(s):  
Visual Detection ◽  
Fusarium Proliferatum ◽  
Recombinase Polymerase Amplification ◽  
G Quadruplex

Asymmetric recombinase polymerase amplification and hemin/G-quadruplex DNAzyme-based visual detection of F. proliferatum is demonstrated.

Development of recombinase polymerase amplification combined with lateral flow detection assay for rapid and visual detection of Ralstonia solanacearum in tobacco

Plant Disease ◽  
2021 ◽  
Author(s):  
Changfeng Li ◽  
Yuliang Ju ◽  
Xun Wu ◽  
Pengfei Shen ◽  
Le Cao ◽  
...  
Keyword(s):  
Ralstonia Solanacearum ◽  
Visual Detection ◽  
High Sensitivity ◽  
High Specificity ◽  
Lateral Flow ◽  
Recombinase Polymerase Amplification ◽  
Molecular Diagnostic ◽  
Tobacco Stem ◽  
Detection Assay ◽  
Flow Detection

Bacterial wilt caused by Ralstonia solanacearum is a serious soil-borne disease that results in severe losses to tobacco (Nicotiana tabacum) production in China. In this study, a novel RPA-LFD assay for the rapid visual detection of R. solanacearum was established using recombinase polymerase amplification (RPA) and lateral-flow dipstick (LFD). The RPA-LFD assay was performed at 37°C in 30 min without complex equipment. Targeting the sequence of the RipTALI-9 gene, we designed RPA primers (Rs-rpa-F/R) and an LF probe (Rs-LF-probe) that showed high specificity to R. solanacearum. The sensitivity of RPA-LFD assay to R. solanacearum was the same as that in conventional PCR at 1 pg genomic DNA, 102 CFU/g artificially inoculated tobacco stem, and 103 CFU/g artificially inoculated soil. The RPA-LFD assay could also detect R. solanacearum from plant and soil samples collected from naturally infested tobacco fields. These results suggest that the RPA-LFD assay developed in this study is a rapid, accurate molecular diagnostic tool with high sensitivity for the detection of R. solanacearum.

A G-quadruplex DNAzyme-based LAMP biosensing platform for a novel colorimetric detection ofListeria monocytogenes

2018 ◽  
Vol 10 (8) ◽  
pp. 848-854 ◽  
Author(s):  
Zhanmin Liu ◽  
Chenhui Yao ◽  
Yanming Wang ◽  
Cuiyun Yang
Keyword(s):  
Listeria Monocytogenes ◽  
Visual Detection ◽  
Colorimetric Detection ◽  
G Quadruplex ◽  
Oflisteria Monocytogenes ◽  
Cascade Amplification

A LAMP-based method for the visual detection ofListeria monocytogeneshas been developed by employing DNAzyme-catalyzed cascade amplification of the colorimetric signal.

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