scholarly journals A gold nanoparticle strip for simultaneously evaluating FMDV immunized antibody level and discriminating FMDV vaccinated animals from infected animals

RSC Advances ◽  
2019 ◽  
Vol 9 (52) ◽  
pp. 30164-30170
Author(s):  
Suzhen Yang ◽  
Yaning Sun ◽  
Jifei Yang ◽  
Yunchao Liu ◽  
Hua Feng ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Antibody Level ◽  
Fmdv Type

A gold nanoparticle strip was developed for rapidly evaluating FMDV type O antibody level and simultaneously discriminating FMDV vaccinated animals from infected animals.

Dielectrophoretic Assembly of Gold Nanoparticle Arrays Evaluated in Terms of Room-Temperature Resistance

2020 ◽  
Vol E103.C (2) ◽  
pp. 62-65
Author(s):  
Yoshinao MIZUGAKI ◽  
Makoto MORIBAYASHI ◽  
Tomoki YAGAI ◽  
Masataka MORIYA ◽  
Hiroshi SHIMADA ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Room Temperature ◽  
Nanoparticle Arrays ◽  
Temperature Resistance

Colorimetric Detection with Aptamer-Gold Nanoparticle Conjugates: Effect of Aptamer Length on Response

2012 ◽  
Author(s):  
Jorge L. Chavez ◽  
Nancy Kelley-Loughnane ◽  
Morley O. Stone ◽  
Robert I. MacCuspie
Keyword(s):  
Gold Nanoparticle ◽  
Colorimetric Detection

Gold Nanoparticle Biosynthesis by E. coli and Conjugation with Streptomycin and Evaluation of its Antibacterial Effect

2014 ◽  
Vol 10 (4) ◽  
pp. 553-561 ◽  
Author(s):  
Samaneh Mazdeh ◽  
Hossein Motamedi ◽  
Azim Khiavi ◽  
Mohammad Mehrabi
Keyword(s):  
Gold Nanoparticle ◽  
Antibacterial Effect ◽  
E Coli ◽  
Nanoparticle Biosynthesis

A Novel Strategy for Synthesis of Gold Nanoparticle Self Assemblies

2014 ◽  
Vol 10 (5) ◽  
pp. 670-675 ◽  
Author(s):  
Jyoti Verma ◽  
Sumit Lal ◽  
Henk Veen ◽  
Cornelis Noorden
Keyword(s):  
Gold Nanoparticle ◽  
Novel Strategy

Interference of Gold Nanoparticles with In vitro Endotoxin Detection Assays

2020 ◽  
Vol 16 (2) ◽  
pp. 204-213 ◽  
Author(s):  
Melissa A. Vetten ◽  
Mary Gulumian
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Surface Reactivity ◽  
In Vitro Assays ◽  
Limulus Amebocyte Lysate ◽  
Chromogenic Assay ◽  
Nanoparticle Suspensions ◽  
Endotoxin Contamination ◽  
Factor C

Background: Endotoxin-free engineered nanoparticle suspensions are imperative for their successful applications in the field of nanomedicine as well as in the investigations in their toxicity. Gold nanoparticles are known to interfere with various in vitro assays due to their optical properties and potential for surface reactivity. In vitro endotoxin testing assays are known to be susceptible to interference caused by the sample being tested. Objective: This study aimed to identify a preferred assay for the testing of endotoxin contamination in gold nanoparticle suspensions. Methods: The interference by gold nanoparticles on three assays namely, the commonly used limulus amebocyte lysate chromogenic assay, the limulus amebocyte lysate gel-clot method, and the less common recombinant Factor C (rFC) assay, was tested. Results: Possible interference could be observed with all three assays. The interference with the absorbance- based chromogenic assay could not be overcome by dilution; whilst the qualitative nature of the gel-clot assay excluded the possibility of distinguishing between a false positive result due to enhancement of the sensitivity of the assay, and genuine endotoxin contamination. However, interference with the rFC assay was easily overcome through dilution. Conclusion: The rFC assay is recommended as an option for endotoxin contamination detection in gold nanoparticle suspensions.

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