scholarly journals Study on microwave assisted extraction of chrysophanol and its intervention in biofilm formation of Streptococcus suis

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 28996-29004
Author(s):  
Jing-Wen Bai ◽  
Xing-Ru Chen ◽  
Yang Tang ◽  
Wen-Qiang Cui ◽  
Da-Long Li ◽  
...  

A microwave assisted extraction technology was used to extract chrysophanol from rhubarb.

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 129 ◽  
Author(s):  
Bao Le ◽  
Kirill S. Golokhvast ◽  
Seung Hwan Yang ◽  
Sangmi Sun

The use of green marine seaweed Ulva spp. as foods, feed supplements, and functional ingredients has gained increasing interest. Microwave-assisted extraction technology was employed to improve the extraction yield and composition of Ulva pertusa polysaccharides. The antioxidant activity of ulvan was also evaluated. The impacts of four independent variables, i.e., extraction time (X1, 30 to 60 min), power (X2, 500 to 700 W), water-to-raw-material ratio (X3, 40 to 70), and pH (X4, 5 to 7) were evaluated. The chemical structure of different polysaccharides fractions was investigated via FT-IR and the determination of their antioxidant activities. A response surface methodology based on a Box–Behnken design (BBD) was used to optimize the extraction conditions as follows: extraction time of 43.63 min, power level of 600 W, water-to-raw-material ratio of 55.45, pH of 6.57, and maximum yield of 41.91%, with a desired value of 0.381. Ulvan exerted a strong antioxidant effect against 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and showed reducing power in vitro. Ulvan protected RAW 264.7 cells against H2O2-induced oxidative stress by upregulating the expression and enhancing the activity of oxidative enzymes such as superoxide dismutase (SOD) and superoxide dismutase (CAT). The results suggest that the polysaccharides from U. pertusa might be promising bioactive compounds for commercial use.


2014 ◽  
Vol 1033-1034 ◽  
pp. 329-336
Author(s):  
Xiu Li Zhang ◽  
Chun Li Li ◽  
Xue Yin ◽  
Xin Yun Su ◽  
De Hong Yu

Catalpol, an iridoid glycoside, has been verified to be of neuroprotection and may be a potential agent for the treatment of neurodegenerative disease. The extraction technology of catalpol has been tested in this paper. We used different extraction methods including extraction at room temperature (ERT), heat reflux extraction, Soxhlet extraction, ultrasonic extraction and microwave-assisted extraction (MAE) to evaluat the percentage extraction of catapol fromRehmannia glutinosa Libosch.The extracts were analyzed by high performance liquid chromatography (HPLC). Methanol (100%, v/v) extracted high percentage extraction of catapol compared to ethanol (60%, v/v) which achieved the highest percentage extraction of catapol in different concentration of ethanol. The results showed that the percentage extraction of catalpol fromRehmannia glutinosa Liboschby MAE was more efficient in short time followed by ultrasonic extraction, Soxhlet extraction, heat reflux extraction and ERT methods. MAE has need of 4 min, whereas ultrasonic extraction, Soxhlet extraction, heat reflux extraction and ERT techniques require 70, 140, 180 min and 60 h respectively, to leach higher percentage extraction of catalpol. The time taken by MAE was nearly 18 times less than the ultrasonic extraction, 35 times less than Soxhlet extraction, 45 times less than heat reflux extraction and 900 times less than ERT for the highest percentage extraction of catalpol. The present results showed that the high extraction efficiency and considerable saving of time by MAE were more competent than the conventional extraction techniques.


BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 8060-8081
Author(s):  
Jie Xu ◽  
Jianjun Wu ◽  
Juan Qi ◽  
Juan Li ◽  
Yongju Liu ◽  
...  

Flavonoids were extracted from Phyllostachys heterocycla leaves by adopting microwave-assisted extraction technology. Based on the single factor experiment and Plackett-Burman design results, the extraction process of flavonoids was further optimized using the response surface methodology. The optimum conditions were as follows: an ethanol concentration of 78.1%, an extraction time of 24.9 min, and a microwave power of 559 W. Under these conditions, the extraction yield of flavonoids was 4.67%, which was in close proximity to the predicted value (4.70%) and higher than the extraction yield from traditional Soxhlet extraction (3.35%). Moreover, the possible extraction mechanisms of these two extraction methods were further derived to explain why the microwave-assisted extraction of flavonoids was more efficient compared with traditional Soxhlet extraction. Ultimately, the antioxidant activities in vitro of flavonoids from Phyllostachys heterocycla leaves were evaluated via DPPH and ABTS radical scavenging assay. The flavonoids from Phyllostachys heterocycla leaves exhibited excellent antioxidant activities in vitro and Phyllostachys heterocycla leaves could be a new natural source for developing antioxidants. Overall, the findings of this research could provide a theoretical reference for the further comprehensive development and utilization of bamboo resources.


2014 ◽  
Vol 1004-1005 ◽  
pp. 868-872
Author(s):  
Yi Hua Jiang ◽  
Xin Long Jiang ◽  
Cheng Gang Cai

Orthogonal experiment optimization of microwave-assisted extraction (MAE) conditions of total flavonoids from castor leaves with the factors of ethanol concentration, microwave power, extraction time, solid/liquid ratio on the yield of total flavonoids were studied. The results showed that optimum extracting conditions of total flavonoids from castor leaves were as follows: adding 1:50 g·mL-1 of 40% alcohol and extracting for 3 min,microwave irradiation power 464W. Microwave extraction of total flavonoids from castor leaves is fast, energy saving and high extraction rate.


2012 ◽  
Vol 518-523 ◽  
pp. 3931-3937 ◽  
Author(s):  
Zhi Ling Chen ◽  
Jian Ping Chao ◽  
Bing Kun Wang ◽  
Hui Lin Cao ◽  
Song Wang ◽  
...  

spinach contains a variety of plant pigments, high lutein content, which makes the research of spinach pigment extracting research special significance. Supercritical CO2fluid extraction food industry is a new extraction and isolation technique; microwave assisted extraction technology has the potential to become future food industrialization production of the leading. The UV-vis spectrophotometric analysis, parallel experimental method to determine the optimum process conditions of extracting pigment from spinach. For supercritical CO2extraction: ethanol (as extractant) is 250ml, extraction pressure 30MPa, extraction temperature 50°C, extraction time 1.5h. The microwave assisted extraction of ethanol as extractant, liquid ratio is 1:20, extraction time1.5h, extraction temperature of 40 °C. The comparison of two methods: microwave extraction is energy saving and high efficiency, and supercritical fluid extraction has safety and environmental protection, therefore, the two methods are worth to advocate and promote.


2010 ◽  
Vol 30 (5) ◽  
pp. 567-568
Author(s):  
Xiao-li LI ◽  
Ming-yuan ZHANG ◽  
Wei-quan ZHAO ◽  
Man Li ◽  
Hai-ying TENG ◽  
...  

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