scholarly journals Functionalized gold nanostructures: promising gene delivery vehicles in cancer treatment

RSC Advances ◽  
2019 ◽  
Vol 9 (41) ◽  
pp. 23894-23907 ◽  
Author(s):  
Sanjay Kumar ◽  
Anchita Diwan ◽  
Parinita Singh ◽  
Shikha Gulati ◽  
Devanshu Choudhary ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Cancer Treatment ◽  
Gold Nanostructures ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

Various functions of gold nanoparticles in conjugation with nucleic acids.

scholarly journals Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles

2014 ◽  
Vol 2 (46) ◽  
pp. 8142-8153 ◽  
Author(s):  
Cheng-Hsiang Kuo ◽  
Lorraine Leon ◽  
Eun Ji Chung ◽  
Ru-Ting Huang ◽  
Timothy J. Sontag ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nucleic Acids ◽  
Enzymatic Degradation ◽  
Polyelectrolyte Complex ◽  
Specific Interactions ◽  
Delivery Vehicles ◽  
Complex Micelles ◽  
Gene Delivery Vehicles

Polyelectrolyte complex micelles have great potential as gene delivery vehicles because of their ability to encapsulate charged nucleic acids forming a core by neutralizing their charge, while simultaneously protecting the nucleic acids from non-specific interactions and enzymatic degradation.

scholarly journals Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Pharmaceutics ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 53
Author(s):  
Calrin Joseph ◽  
Aliscia Daniels ◽  
Sooboo Singh ◽  
Moganavelli Singh
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Folate Receptor ◽  
Endosomal Escape ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

Nanotechnology has emerged as a promising treatment strategy in gene therapy, especially against diseases such as cancer. Gold nanoparticles (AuNPs) are regarded as favorable gene delivery vehicles due to their low toxicity, ease of synthesis and ability to be functionalized. This study aimed to prepare functionalized AuNPs (FAuNPs) and evaluate their folate-targeted and nontargeted pCMV-Luc-DNA delivery in breast cancer cells in vitro. CS was added to induce stability and positive charges to the AuNPs (Au-CS), histidine (Au-CS-His) to enhance endosomal escape and folic acid for folate-receptor targeting (Au-CS-FA-His). The FAuNP:pDNA nanocomplexes possessed favorable sizes (<135 nm) and zeta potentials (<−20 mV), strong compaction efficiency and were capable of pDNA protection against nuclease degradation. These nanocomplexes showed minimal cytotoxicity (>73% cell viability) and enhanced transgene activity. The influence of His was notable in the HER2 overexpressing SKBR3 cells, which produced higher gene expression. Furthermore, the FA-targeted nanocomplexes enhanced receptor-mediated endocytosis, especially in MCF-7 cells, as confirmed by the receptor competition assay. While the role of His may need further optimization, the results achieved suggest that these FAuNPs may be suitable gene delivery vehicles for breast cancer therapeutics.

Exosome as a Natural Gene Delivery Vector for Cancer Treatment

2020 ◽  
Vol 20 (11) ◽  
pp. 821-830
Author(s):  
Prasad Pofali ◽  
Adrita Mondal ◽  
Vaishali Londhe
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Cancer Treatment ◽  
Intestinal Barrier ◽  
Gene Carrier ◽  
Gene Delivery Vector ◽  
Delivery Vector

Background: Current gene therapy vectors such as viral, non-viral, and bacterial vectors, which are used for cancer treatment, but there are certain safety concerns and stability issues of these conventional vectors. Exosomes are the vesicles of size 40-100 nm secreted from multivesicular bodies into the extracellular environment by most of the cell types in-vivo and in-vitro. As a natural nanocarrier, exosomes are immunologically inert, biocompatible, and can cross biological barriers like the blood-brain barrier, intestinal barrier, and placental barrier. Objective: This review focusses on the role of exosome as a carrier to efficiently deliver a gene for cancer treatment and diagnosis. The methods for loading of nucleic acids onto the exosomes, advantages of exosomes as a smart intercellular shuttle for gene delivery and therapeutic applications as a gene delivery vector for siRNA, miRNA and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and also the limitations of exosomes as a gene carrier are all reviewed in this article. Methods: Mostly, electroporation and chemical transfection are used to prepare gene loaded exosomes. Results: Exosome-mediated delivery is highly promising and advantageous in comparison to the current delivery methods for systemic gene therapy. Targeted exosomes, loaded with therapeutic nucleic acids, can efficiently promote the reduction of tumor proliferation without any adverse effects. Conclusion: In the near future, exosomes can become an efficient gene carrier for delivery and a biomarker for the diagnosis and treatment of cancer.

Characterization of Cationic Vector-Based Gene Delivery Vehicles Using Isothermal Titration and Differential Scanning Calorimetry

2003 ◽  
pp. 319-348 ◽  
Author(s):  
Brian A. Lobo ◽  
Sheila A. Rogers ◽  
Christopher M. Wiethoff ◽  
Sirirat Choosakoonkriang ◽  
Susan Bogdanowich-Knipp ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Gene Delivery ◽  
Scanning Calorimetry ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

scholarly journals Functionalized MoS2 Nanosheets as Multi-Gene Delivery Vehicles for In Vivo Pancreatic Cancer Therapy

2018 ◽  
Vol 2 (4) ◽  
pp. 371-386 ◽  
Author(s):  
Feng Yin ◽  
Tommy Anderson ◽  
Nishtha Panwar ◽  
Kang Zhang ◽  
Swee Chuan Tjin ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Mos2 Nanosheets ◽  
Delivery Vehicles ◽  
Pancreatic Cancer Therapy ◽  
Gene Delivery Vehicles
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