scholarly journals Anionic nanoparticle-lipid membrane interactions: the protonation of anionic ligands at the membrane surface reduces membrane disruption

RSC Advances ◽  
2019 ◽  
Vol 9 (25) ◽  
pp. 13992-13997 ◽  
Author(s):  
Sebastian Salassi ◽  
Ester Canepa ◽  
Riccardo Ferrando ◽  
Giulia Rossi
Keyword(s):  
Lipid Membranes ◽  
Au Nanoparticles ◽  
Lipid Membrane ◽  
Membrane Surface ◽  
Membrane Disruption ◽  
Membrane Interactions ◽  
Carboxylate Groups ◽  
Model Lipid Membranes ◽  
Anionic Ligands

The interaction between anionic Au nanoparticles and model lipid membranes is facilitated by the spontaneous protonation of the NP ligand carboxylate groups, COO−˙ → COOH, in the lipid headgroup region.

scholarly journals Interaction of Tau Protein with Model Lipid Membranes Induces Tau Structural Compaction and Membrane Disruption

Biochemistry ◽  
2012 ◽  
Vol 51 (12) ◽  
pp. 2539-2550 ◽  
Author(s):  
Emmalee M. Jones ◽  
Manish Dubey ◽  
Phillip J. Camp ◽  
Briana C. Vernon ◽  
Jacek Biernat ◽  
...  
Keyword(s):  
Tau Protein ◽  
Lipid Membranes ◽  
Membrane Disruption ◽  
Model Lipid Membranes

scholarly journals Structural Study of the Hydration of Lipid Membranes Upon Interaction With Mesoporous Supports Prepared by Standard Methods and/or X‐Ray Irradiation

2021 ◽  
Vol 8 ◽  
Author(s):  
Benedetta Marmiroli ◽  
Barbara Sartori ◽  
Adriana R. Kyvik ◽  
Imma Ratera ◽  
Heinz Amenitsch
Keyword(s):  
Structural Study ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Subsequent Development ◽  
Grazing Incidence ◽  
Mesoporous Films ◽  
X Ray ◽  
Sensing Applications ◽  
Model Lipid Membranes ◽  
Biological Studies

Mesoporous materials feature ordered tailored structures with uniform pore sizes and highly accessible surface areas, making them an ideal host for functional organic molecules or nanoparticles for analytical and sensing applications. Moreover, as their porosity could be employed to deliver fluids, they could be suitable materials for nanofluidic devices. As a first step in this direction, we present a study of the hydration of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) model lipid membranes on solid mesoporous support. POPC was selected as it changes the structure upon hydration at room temperature. Mesoporous films were prepared using two different templating agents, Pluronic P123 (PEO–PPO–PEO triblock copolymer where PEO is polyethylene oxide and PPO is polypropylene oxide) and Brij 58 (C16H33(EO)20OH where EO is ethylene oxide), both following the conventional route and by X-ray irradiation via deep X-ray lithography technique and subsequent development. The same samples were additionally functionalized with a self-assembly monolayer (SAM) of (3-aminopropyl)triethoxysilane. For every film, the contact angle was measured. A time resolved structural study was conducted using in situ grazing incidence small-angle X-ray scattering while increasing the external humidity (RH), from 15 to 75% in a specially designed chamber. The measurements evidenced that the lipid membrane hydration on mesoporous films occurs at a lower humidity value with respect to POPC deposited on silicon substrates, demonstrating the possibility of using porosity to convey water from below. A different level of hydration was reached by using the mesoporous thin film prepared with conventional methods or the irradiated ones, or by functionalizing the film using the SAM strategy, meaning that the hydration can be partially selectively tuned. Therefore, mesoporous films can be employed as “interactive” sample holders with specimens deposited on them. Moreover, thanks to the possibility of patterning the films using deep X-ray lithography, devices for biological studies of increasing complexity by selectively functionalizing the mesopores with biofunctional SAMs could be designed and fabricated.

scholarly journals Diptool—A Novel Numerical Tool for Membrane Interactions Analysis, Applying to Antimicrobial Detergents and Drug Delivery Aids

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6455
Author(s):  
Mateusz Rzycki ◽  
Sebastian Kraszewski ◽  
Marta Gładysiewicz-Kudrawiec
Keyword(s):  
Drug Delivery ◽  
Free Energy ◽  
Antimicrobial Agents ◽  
Lipid Membrane ◽  
Gemini Surfactants ◽  
Experimental Studies ◽  
Critical Issue ◽  
Membrane Interactions ◽  
Bacterial Strains ◽  
Model Lipid Membranes

The widespread problem of resistance development in bacteria has become a critical issue for modern medicine. To limit that phenomenon, many compounds have been extensively studied. Among them were derivatives of available drugs, but also alternative novel detergents such as Gemini surfactants. Over the last decade, they have been massively synthesized and studied to obtain the most effective antimicrobial agents, as well as the most selective aids for nanoparticles drug delivery. Various protocols and distinct bacterial strains used in Minimal Inhibitory Concentration experimental studies prevented performance benchmarking of different surfactant classes over these last years. Motivated by this limitation, we designed a theoretical methodology implemented in custom fast screening software to assess the surfactant activity on model lipid membranes. Experimentally based QSAR (quantitative structure-activity relationship) prediction delivered a set of parameters underlying the Diptool software engine for high-throughput agent-membrane interactions analysis. We validated our software by comparing score energy profiles with Gibbs free energy from the Adaptive Biasing Force approach on octenidine and chlorhexidine, popular antimicrobials. Results from Diptool can reflect the molecule behavior in the lipid membrane and correctly predict free energy of translocation much faster than classic molecular dynamics. This opens a new venue for searching novel classes of detergents with sharp biologic activity.

scholarly journals Evaluation of Strategies for Improving Proteolytic Resistance of Antimicrobial Peptides by Using Variants of EFK17, an Internal Segment of LL-37

2008 ◽  
Vol 53 (2) ◽  
pp. 593-602 ◽  
Author(s):  
Adam A. Strömstedt ◽  
Mukesh Pasupuleti ◽  
Artur Schmidtchen ◽  
Martin Malmsten
Keyword(s):  
Lipid Membranes ◽  
Lipid Membrane ◽  
Antimicrobial Properties ◽  
Peptide Sequence ◽  
Proteolytic Degradation ◽  
Human Neutrophil Elastase ◽  
Helix Formation ◽  
Proteolytic Resistance ◽  
Model Lipid Membranes ◽  
Protease Cleavage Sites

ABSTRACT Methods for increasing the proteolytic stability of EFK17 (EFKRIVQRIKDFLRNLV), a new peptide sequence with antimicrobial properties derived from LL-37, were evaluated. EFK17 was modified by four d-enantiomer or tryptophan (W) substitutions at known protease cleavage sites as well as by terminal amidation and acetylation. The peptide variants were studied in terms of proteolytic resistance, antibacterial potency, and cytotoxicity but also in terms their adsorption at model lipid membranes, liposomal leakage generation, and secondary-structure behavior. The W substitutions resulted in a marked reduction in the proteolytic degradation caused by human neutrophil elastase, Staphylococcus aureus aureolysin, and V8 protease but not in the degradation caused by Pseudomonas aeruginosa elastase. For the former two endoproteases, amidation and acetylation of the terminals also reduced proteolytic degradation but only when used in combination with W substitutions. The d-enantiomer substitutions rendered the peptides indigestible by all four proteases; however, those peptides displayed little antimicrobial potency. The W- and end-modified peptides, on the other hand, showed an increased bactericidal potency compared to that of the native peptide sequence, coupled with a moderate cytotoxicity that was largely absent in serum. The bactericidal, cytotoxic, and liposome lytic properties correlated with each other as well as with the amount of peptide adsorbed at the lipid membrane and the extent of helix formation associated with the adsorption. The lytic properties of the W-substituted peptides were less impaired by increased ionic strength, presumably by a combination of W-mediated stabilization of the largely amphiphilic helix conformation and a nonelectrostatic W affinity for the bilayer interface. Overall, W substitutions constitute an interesting means to reduce the proteolytic susceptibility of EFK17 while also improving antimicrobial performance.

scholarly journals Induction of Morphological Changes in Model Lipid Membranes and the Mechanism of Membrane Disruption by a Large Scorpion-Derived Pore-Forming Peptide

2005 ◽  
Vol 89 (6) ◽  
pp. 4067-4080 ◽  
Author(s):  
Kaoru Nomura ◽  
Gilles Ferrat ◽  
Terumi Nakajima ◽  
Herve Darbon ◽  
Takashi Iwashita ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Morphological Changes ◽  
Membrane Disruption ◽  
Model Lipid Membranes

scholarly journals Responsive core-shell DNA particles trigger lipid-membrane disruption and bacteria entrapment

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Michal Walczak ◽  
Ryan A. Brady ◽  
Leonardo Mancini ◽  
Claudia Contini ◽  
Roger Rubio-Sánchez ◽  
...  
Keyword(s):  
Immune Cells ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Lipid Vesicles ◽  
Membrane Disruption ◽  
Dna Nanostructures ◽  
E Coli ◽  
Synthetic Dna ◽  
Amyloid Aggregates ◽  
Particle Coalescence

AbstractBiology has evolved a variety of agents capable of permeabilizing and disrupting lipid membranes, from amyloid aggregates, to antimicrobial peptides, to venom compounds. While often associated with disease or toxicity, these agents are also central to many biosensing and therapeutic technologies. Here, we introduce a class of synthetic, DNA-based particles capable of disrupting lipid membranes. The particles have finely programmable size, and self-assemble from all-DNA and cholesterol-DNA nanostructures, the latter forming a membrane-adhesive core and the former a protective hydrophilic corona. We show that the corona can be selectively displaced with a molecular cue, exposing the ‘sticky’ core. Unprotected particles adhere to synthetic lipid vesicles, which in turn enhances membrane permeability and leads to vesicle collapse. Furthermore, particle-particle coalescence leads to the formation of gel-like DNA aggregates that envelop surviving vesicles. This response is reminiscent of pathogen immobilisation through immune cells secretion of DNA networks, as we demonstrate by trapping E. coli bacteria.

scholarly journals Impact of selected polyphenolics on the structural properties of model lipid membranes – a review

2017 ◽  
Vol 6 (2) ◽  
Author(s):  
Nataša P. Ulrih ◽  
Ajda Ota ◽  
Veronika Abram
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Fluidity ◽  
Phenolic Acids ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Biological Effects ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Model Lipid Membranes ◽  
Electron Paramagnetic

This review is a presentation of data gathered on the interactions of several polyphenolics (i.e., phenolic acids, stilbenes, flavonoids) with lipid bilayers of different lipid compositions. These polyphenolics have been investigated through a combination of fluorescence spectroscopy, electron paramagnetic resonance spectroscopy, and differential scanning calorimetry, to detect changes in membrane fluidity. Among the investigated phenolic acids, the least polar phenolic acid, p-coumaric acid, has the greatest effect on lipid membrane structure. It appears to have a greater ability to cross membranes by passive transport than more polar phenolic acids. On the other hand, among the flavonoids that have been studied, the anthocyanins cyanidin 3-glucoside and its aglycone are inactive. All of the flavonols tested, except for epigallocatechin-3-gallate, promote small decreases in membrane fluidity. Computer simulation of electron paramagnetic resonance spectra for flavonoids indicated two or three regions in the phosphatidylcholine/ phosphatidylserine (2.4:1) membrane with different fluidity characteristics. The effects of the different flavonoids are correlated to their structural characteristics, whereby even the difference in one -OH group can be important, as can the number of H-bonds they form. The role of membrane composition and flavonoid structure in these interactions with lipid membranes are of great importance for bioavailability of these compounds and for their biological effects in an organism

scholarly journals Rapid evaluation of gold nanoparticle–lipid membrane interactions using a lipid/polydiacetylene vesicle sensor

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 3049-3055
Author(s):  
Congcong Gu ◽  
Yingying Geng ◽  
Feng Zheng ◽  
Vincent M. Rotello
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Rapid Evaluation ◽  
Membrane Interactions

Lipid/polydiacetylene vesicles serve as a sensor to rapidly measure the interactions between gold nanoparticles and lipid membranes. A colorimetric signal is generated upon membrane disturbance caused by gold nanoparticles.

scholarly journals Structure, Formation, and Biological Interactions of Supported Lipid Bilayers (SLB) Incorporating Lipopolysaccharide

Coatings ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 981
Author(s):  
Palak Sondhi ◽  
Dhanbir Lingden ◽  
Keith J. Stine
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Lipid Membrane ◽  
Biologically Active ◽  
Supported Lipid Bilayers ◽  
Biological Interactions ◽  
Relevant Model ◽  
Biologically Relevant ◽  
Model Lipid Membranes ◽  
Biomimetic Membrane

Biomimetic membrane systems play a crucial role in the field of biosensor engineering. Over the years, significant progress has been achieved creating artificial membranes by various strategies from vesicle fusion to Langmuir transfer approaches to meet an ever-growing demand for supported lipid bilayers on various substrates such as glass, mica, gold, polymer cushions, and many more. This paper reviews the diversity seen in the preparation of biologically relevant model lipid membranes which includes monolayers and bilayers of phospholipid and other crucial components such as proteins, characterization techniques, changes in the physical properties of the membranes during molecular interactions and the dynamics of the lipid membrane with biologically active molecules with special emphasis on lipopolysaccharides (LPS).

Sign in / Sign up

Export Citation Format

Share Document