scholarly journals Oxidative stress generated at nickel oxide nanoparticle interface results in bacterial membrane damage leading to cell death

RSC Advances ◽  
2019 ◽  
Vol 9 (43) ◽  
pp. 24888-24894 ◽  
Author(s):  
Nibedita Behera ◽  
Manoranjan Arakha ◽  
Mamali Priyadarshinee ◽  
Biraja S. Pattanayak ◽  
Siba Soren ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Nickel Oxide ◽  
Metal Oxide Nanoparticles ◽  
Membrane Damage ◽  
Oxide Nanoparticles ◽  
Bacterial Membrane ◽  
Antibacterial Effects ◽  
Nickel Oxide Nanoparticle ◽  
Oxide Nanoparticle

Metal oxide nanoparticles (NPs) have shown enhanced antibacterial effects against many bacteria.

scholarly journals Phytosynthetic Fabrication of Lanthanum Ion-Doped Nickel Oxide Nanoparticles Using Sesbania grandiflora Leaf Extract and Their Anti-Microbial Properties

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 124
Author(s):  
Srihasam Saiganesh ◽  
Thyagarajan Krishnan ◽  
Golla Narasimha ◽  
Hesham S. Almoallim ◽  
Sulaiman Ali Alhari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Optical Properties ◽  
Rare Earth ◽  
Nickel Oxide ◽  
Photoelectron Spectroscopy ◽  
Metal Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Nio Nanoparticles ◽  
X Ray ◽  
Sesbania Grandiflora

Over the past few years, the photogenic fabrication of metal oxide nanoparticles has attracted considerable attention, owing to the simple, eco-friendly, and non-toxic procedure. Herein, we fabricated NiO nanoparticles and altered their optical properties by doping with a rare earth element (lanthanum) using Sesbania grandiflora broth for antibacterial applications. The doping of lanthanum with NiO was systematically studied. The optical properties of the prepared nanomaterials were investigated through UV-Vis diffuse reflectance spectra (UV-DRS) analysis, and their structures were studied using X-ray diffraction analysis. The morphological features of the prepared nanomaterials were examined by scanning electron microscopy and transmission electron microscopy, their elemental structure was analyzed by energy-dispersive X-ray spectral analysis, and their oxidation states were analyzed by X-ray photoelectron spectroscopy. Furthermore, the antibacterial action of NiO and La-doped NiO nanoparticles was studied by the zone of inhibition method for Gram-negative and Gram-positive bacterial strains such as Escherichia coli and Bacillus sublitis. It was evident from the obtained results that the optimized compound NiOLa-04 performed better than the other prepared compounds. To the best of our knowledge, this is the first report on the phytosynthetic fabrication of rare-earth ion Lanthanum (La3+)-doped Nickel Oxide (NiO) nanoparticles and their anti-microbial studies.

Epicatechin and its in vivo metabolite, 3′-O-methyl epicatechin, protect human fibroblasts from oxidative-stress-induced cell death involving caspase-3 activation

2001 ◽  
Vol 354 (3) ◽  
pp. 493-500 ◽  
Author(s):  
Jeremy P. E. SPENCER ◽  
Hagen SCHROETER ◽  
Gunter KUHNLE ◽  
S. Kaila S. SRAI ◽  
Rex M. TYRRELL ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Cell Death ◽  
Caspase 3 ◽  
Cell Damage ◽  
Protective Action ◽  
Human Fibroblasts ◽  
Membrane Damage ◽  
Antioxidant Properties

There is considerable current interest in the cytoprotective effects of natural antioxidants against oxidative stress. In particular, epicatechin, a major member of the flavanol family of polyphenols with powerful antioxidant properties in vitro, has been investigated to determine its ability to attenuate oxidative-stress-induced cell damage and to understand the mechanism of its protective action. We have induced oxidative stress in cultured human fibroblasts using hydrogen peroxide and examined the cellular responses in the form of mitochondrial function, cell-membrane damage, annexin-V binding and caspase-3 activation. Since one of the major metabolites of epicatechin in vivo is 3′-O-methyl epicatechin, we have compared its protective effects with that of epicatechin. The results provide the first evidence that 3′-O-methyl epicatechin inhibits cell death induced by hydrogen peroxide and that the mechanism involves suppression of caspase-3 activity as a marker for apoptosis. Furthermore, the protection elicited by 3′-O-methyl epicatechin is not significantly different from that of epicatechin, suggesting that hydrogen-donating antioxidant activity is not the primary mechanism of protection.

scholarly journals Gas Sensing with Iridium Oxide Nanoparticle Decorated Carbon Nanotubes

Sensors ◽  
2018 ◽  
Vol 19 (1) ◽  
pp. 113 ◽  
Author(s):  
Juan Casanova-Cháfer ◽  
Eric Navarrete ◽  
Xavier Noirfalise ◽  
Polona Umek ◽  
Carla Bittencourt ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Gas Sensing ◽  
Metal Oxide Nanoparticles ◽  
Iridium Oxide ◽  
Oxide Nanoparticles ◽  
Step Method ◽  
Experimental Conditions ◽  
Multi Wall Carbon Nanotubes ◽  
Condensation Growth ◽  
Oxide Nanoparticle

The properties of multi-wall carbon nanotubes decorated with iridium oxide nanoparticles (IrOx-MWCNTs) are studied to detect harmful gases such as nitrogen dioxide and ammonia. IrOx nanoparticles were synthetized using a two-step method, based on a hydrolysis and acid condensation growth mechanism. The metal oxide nanoparticles obtained were employed for decorating the sidewalls of carbon nanotubes. Iridium-oxide nanoparticle decorated carbon nanotube material showed higher and more stable responses towards NH3 and NO2 than bare carbon nanotubes under different experimental conditions, establishing the optimal operating temperatures and estimating the limits of detection and quantification. Furthermore, the nanomaterials employed were studied using different morphological and compositional characterization techniques and a gas sensing mechanism is proposed.

scholarly journals In Situ Transmission Electron Microscopy Graphene Liquid Cell on Chemical Sodiation of Nickel Oxide Nanoparticle

2017 ◽  
Vol 23 (S1) ◽  
pp. 204-205
Author(s):  
Frank Jaksoni Mweta ◽  
Joon Ha Chang ◽  
Hyeon Kook Seo ◽  
Sung Joo Kim ◽  
Jun Young Cheong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nickel Oxide ◽  
Transmission Electron ◽  
Nickel Oxide Nanoparticle ◽  
Liquid Cell ◽  
Oxide Nanoparticle

scholarly journals Combinatorial Antimicrobial Efficacy and Mechanism of Linalool Against Clinically Relevant Klebsiella pneumoniae

2021 ◽  
Vol 12 ◽  
Author(s):  
Shun-Kai Yang ◽  
Khatijah Yusoff ◽  
Mokrish Ajat ◽  
Chien-Yeong Wee ◽  
Polly-Soo-Xi Yap ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Klebsiella Pneumoniae ◽  
Membrane Permeability ◽  
Membrane Damage ◽  
Bacterial Membrane ◽  
Bacterial Cells ◽  
Bacterial Killing ◽  
Potential Measurement ◽  
Bacterial Surface ◽  
Electron Microscopies

Antibiotic–adjuvant combinatory therapy serves as a viable treatment option in addressing antibiotic resistance in the clinical setting. This study was carried out to assess and characterize the adjuvant potential and mode of action of linalool against carbapenemase-producing Klebsiella pneumoniae (KPC-KP). Linalool exhibited bactericidal activity alone (11,250 μg/ml) and in combination with meropenem (5,625 μg/ml). Comparative proteomic analysis showed significant reduction in the number of cytoplasmic and membrane proteins, indicating membrane damage in linalool-treated KPC-KP cells. Upregulation of oxidative stress regulator proteins and downregulation of oxidative stress-sensitive proteins indicated oxidative stress. Zeta potential measurement and outer membrane permeability assay revealed that linalool increases the bacterial surface charge as well as the membrane permeability. Intracellular leakage of nucleic acid and proteins was detected upon linalool treatment. Scanning and transmission electron microscopies further revealed the breakage of bacterial membrane and loss of intracellular materials. Linalool induced oxidative stress by generating reactive oxygen species (ROS) which initiates lipid peroxidation, leading to damage of the bacterial membrane. This leads to intracellular leakage, eventually killing the KPC-KP cells. Our study demonstrated that linalool possesses great potential in future clinical applications as an adjuvant along with existing antibiotics attributed to their ability in disrupting the bacterial membrane by inducing oxidative stress. This facilitates the uptake of antibiotics into the bacterial cells, enhancing bacterial killing.

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