scholarly journals Identification and characterization of differentially expressed miRNAs in HepG2 cells under normoxic and hypoxic conditions

RSC Advances ◽  
2019 ◽  
Vol 9 (29) ◽  
pp. 16884-16891 ◽  
Author(s):  
Fanzhi Kong ◽  
Wei Ran ◽  
Ning Jiang ◽  
Shize Li ◽  
Dongjie Zhang ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Transcriptional Regulators ◽  
Differentially Expressed ◽  
Hypoxic Conditions ◽  
Differentially Expressed Mirnas ◽  
Identification And Characterization

MicroRNAs (miRNAs) are important post-transcriptional regulators involved in hypoxia conditions; however, their roles in HepG2 cells remain poorly understood.

Identification and characterization of microRNAs in white and black coated yak

2018 ◽  
Author(s):  
Wang-Dui Basang ◽  
Tian-Wu An ◽  
Xiao-Lin Luo ◽  
Yan-Bin Zhu ◽  
Luo-Bu Danjiu Danjiu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Coat Color ◽  
Wnt Signaling Pathway ◽  
Differentially Expressed ◽  
Black And White ◽  
Kegg Pathways ◽  
Differentially Expressed Mirnas ◽  
Paired End Sequencing ◽  
Identification And Characterization

In this study, we used high-throughput technology to provide the first transcriptome dataset for differentially expressed miRNA in mixed pools of dermis tissue from black- and white-coated yak to research the possible molecular mechanisms of yak coat pigmentation. In this study, 92,636,002 and 95,917,842 clear reads were generated through Illumina paired-end sequencing. A total of 78 differentially expressed miRNAs (DEMs) were identified, including 59 upregulated and 19 downregulated miRNAs in the mixed pools of white-coated yak compared with the mixed pools of black-coated yak. In addition, 3634 genes were predicted as putative targets of DEMs. These DEGs related to 59 GO categories and were enriched in 216 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including melanogenesis and the Wnt signaling pathway. The results of the current study indicated that the coat color of the yak involved the transcriptional regulation process of miRNAs. These results provide helpful data to understand the molecular mechanisms of yak coat pigmentation.

Identification and characterization of differentially expressed miRNAs between bamboo shoot and rhizome shoot

2016 ◽  
Vol 59 (4) ◽  
pp. 322-335 ◽  
Author(s):  
Qun-Ying Jin ◽  
Hua-Zheng Peng ◽  
Er-Pei Lin ◽  
Nan Li ◽  
Dan-Ni Huang ◽  
...  
Keyword(s):  
Differentially Expressed ◽  
Bamboo Shoot ◽  
Differentially Expressed Mirnas ◽  
Identification And Characterization

In silico identification and characterization of sensory motifs in the transcriptional regulators of the ArsR-SmtB family

Metallomics ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1476-1500 ◽  
Author(s):  
Rima Roy ◽  
Saikat Samanta ◽  
Surajit Patra ◽  
Nav Kumar Mahato ◽  
Rudra P. Saha
Keyword(s):  
In Silico ◽  
Transcriptional Regulators ◽  
Transcriptional Repressors ◽  
Structural Elements ◽  
In Silico Identification ◽  
Metal Efflux ◽  
Identification And Characterization ◽  
Secondary Structural Elements ◽  
Efflux Proteins

The ArsR-SmtB family of transcriptional repressors regulates the transcription of metal-efflux proteins by binding specific metals at a variety of secondary structural elements, called motifs, on the surface of the proteins.

scholarly journals MicroRNA expression profiling involved in Borax-induced anti-tumor effect using gene-chip analysis

2020 ◽  
Author(s):  
Lun Wu ◽  
Ying Wei ◽  
Wen-Bo Zhou ◽  
Jiao Zhou ◽  
Li-Hua Yang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Hepg2 Cells ◽  
Enrichment Analysis ◽  
Gene Chip ◽  
Differentially Expressed ◽  
Control Group ◽  
Pathway Enrichment Analysis ◽  
Ras Signaling ◽  
Therapeutic Benefits ◽  
Differentially Expressed Mirnas

Abstract Background Borax, a boron compound, which is becoming widely recognized for its biological effects, including antioxidant activity, cytotoxicity, and potential therapeutic benefits. However, the specific molecular mechanisms underlying borax-induced anti-tumor effect still remain to be to further elucidated. MicroRNAs (miRNAs) may play key roles in cellular processes including tumor progression, cell apoptosis and cytotoxicity. Thus, this study aimed to investigate, whether miRNAs were involved in the borax-mediated anti-tumor effect using miRNA profiling of a human liver cancer cell line (HepG2) using gene-chip analysis.Methods Total RNA was extracted and purified from HepG2 cells that were treated with 4 mM borax for either 2 or 24 h. The samples underwent microarray analysis using an Agilent Human miRNA Array. Differentially expressed miRNAs were analysed by volcano plot and heatmap, and were validated using real-time fluorescent quantitative PCR (qPCR).ResultsAmong this, 2- or 24-h exposure to borax significantly altered the expression level of miRNAs in HepG2 cells, 4 or 14 were upregulated and 3 were downregulated compared with the control group, respectively (≥2-fold; P<0.05). GO enrichment analysis and KEGG pathway enrichment analysis revealed that target genes of differentially expressed miRNAs in HepG2 cells predominantly participated in MAPK signaling pathway, TGF-beta signaling pathway, NF-kappa B signaling pathway, etc; in 2-h borax treatment group, while Ras signaling pathway, FoxO signaling pathway, Cellular senescence, etc; involved in 24-h treatment group.Conclusions Result indicates that borax-induced anti-tumor effect may be associated with alterations in miRNAs.

Identification and Characterization of Two Novel and Differentially Expressed Isoforms of Humanα2- andα1-Globin Genes

Hemoglobin ◽  
2012 ◽  
Vol 36 (5) ◽  
pp. 421-432 ◽  
Author(s):  
Reza Ghassemifar ◽  
Luke Forster ◽  
Talal Qadah ◽  
Jill Finlayson
Keyword(s):  
Differentially Expressed ◽  
Globin Genes ◽  
Identification And Characterization
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