scholarly journals Systematically quantitative proteomics and metabolite profiles offer insight into fruit ripening behavior in Fragaria × ananassa

RSC Advances ◽  
2019 ◽  
Vol 9 (25) ◽  
pp. 14093-14108 ◽  
Author(s):  
Li Li ◽  
Qiong Wu ◽  
Youyong Wang ◽  
Morteza Soleimani Aghdam ◽  
Zhaojun Ban ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Quantitative Proteomics ◽  
Fragaria Ananassa ◽  
Metabolite Profiles ◽  
Insight Into

Proteome and metabolite profiles of fruit ripening behavior in Fragaria × ananassa Duch. ‘Benihoppe’.

scholarly journals Comparative Proteomics Analysis of Mouse Habu Nephritis Models with and without Unilateral Nephrectomy

2016 ◽  
Vol 39 (5) ◽  
pp. 1761-1776 ◽  
Author(s):  
Lei Chen ◽  
Yang Lu ◽  
Jun Wen ◽  
Xu Wang ◽  
Lingling Wu ◽  
...  
Keyword(s):  
Renal Injury ◽  
Quantitative Proteomics ◽  
Mesangial Cells ◽  
Unilateral Nephrectomy ◽  
Label Free ◽  
Proteomics Analysis ◽  
Single Kidney ◽  
Regulation Of Actin Cytoskeleton ◽  
Insight Into

Background/Aims: Individuals possessing a single kidney are at greater risk of renal injury upon exposure to harmful stimuli. This study aimed to explore the pathogenesis of renal injury in glomerulonephritis with versus without unilateral nephrectomy (UNX). Methods: Histological analysis and label-free quantitative proteomics were performed on two models—the Habu snake venom-induced glomerulonephritis model with versus without UNX (HabuU and Habu models, respectively). The role of villin 1, a differentially expressed protein (DEP) in mouse mesangial cells, was investigated. Results: Persistent mesangiolysis and focal hypercellularity together with reduced activation of cell proliferation in the HabuU model induced more serious renal injury compared with that in the Habu model. The DEPs between the two models were identified by label-free liquid chromatography-mass spectrometry. The KEGG pathway results indicated that regulation of actin cytoskeleton and focal adhesion were specifically enriched in the HabuU model. The cytoskeleton regulation protein villin 1 was downregulated in the HabuU model, but unchanged in the Habu model. Knockdown of villin 1 promoted apoptosis and inhibited the proliferation of mouse mesangial cells, suggesting villin 1 to be involved in qlomerular lesion self-repair insufficiency. Conclusion: By assessing the proteomic profiles of the two models, this study identified several important differences, particularly villin 1 expression, in regulatory mechanisms between the two models. Our findings provide novel insight into the mechanism of serious renal injury in glomerulonephritis with UNX.

scholarly journals Transcriptome profiling by RNA-Seq reveals differentially expressed genes related to fruit development and ripening characteristics in strawberries (Fragaria×ananassa)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4976 ◽  
Author(s):  
Panpan Hu ◽  
Gang Li ◽  
Xia Zhao ◽  
Fengli Zhao ◽  
Liangjie Li ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Fruit Ripening ◽  
Fruit Development ◽  
Transcriptome Profiling ◽  
Differentially Expressed ◽  
Fragaria Ananassa ◽  
Mads Box ◽  
Cell Wall Modification ◽  
Ripening Process ◽  
Fruit Development And Ripening

Strawberry (Fragaria × ananassa) is an ideal plant for fruit development and ripening research due to the rapid substantial changes in fruit color, aroma, taste, and softening. To gain deeper insights into the genes that play a central regulatory role in strawberry fruit development and ripening characteristics, transcriptome profiling was performed for the large green fruit, white fruit, turning fruit, and red fruit stages of strawberry. A total of 6,608 differentially expressed genes (DEGs) with 2,643 up-regulated and 3,965 down-regulated genes were identified in the fruit development and ripening process. The DEGs related to fruit flavonoid biosynthesis, starch and sucrose biosynthesis, the citrate cycle, and cell-wall modification enzymes played important roles in the fruit development and ripening process. Particularly, some candidate genes related to the ubiquitin mediated proteolysis pathway and MADS-box were confirmed to be involved in fruit development and ripening according to their possible regulatory functions. A total of fiveubiquitin-conjugating enzymesand 10MADS-box transcription factorswere differentially expressed between the four fruit ripening stages. The expression levels of DEGs relating to color, aroma, taste, and softening of fruit were confirmed by quantitative real-time polymerase chain reaction. Our study provides important insights into the complicated regulatory mechanism underlying the fruit ripening characteristics inFragaria × ananassa.

ABA accelerates blackberry (Rubus spp.) fruit ripening by positively affecting ripening-related gene expression and metabolite profiles

2021 ◽  
pp. 1-16
Author(s):  
Chunhong Zhang ◽  
Yaqiong Wu ◽  
Zhenghao Xiong ◽  
Weilin Li ◽  
Wenlong Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Fruit Ripening ◽  
Plant Hormone ◽  
Expression Profiles ◽  
Related Gene ◽  
Commercial Use ◽  
Metabolite Profiles ◽  
Metabolite Accumulation ◽  
Aba Synthesis

BACKGROUND: The softness of blackberry fruits limits their postharvest shelf-life and commercial use, and abscisic acid (ABA) is considered one of the key hormones involved in fruit ripening. OBJECTIVE: This study aimed to explore the underlying physiological and molecular actions of ABA on blackberry fruit ripening and softening. METHODS: Various physiological indices of and plant hormone levels in treated and untreated blackberry fruits were determined simultaneously. The differentially expressed genes (DEGs) were analyzed by RNA-sequencing, and their expression profiles were detected. The ripening mechanism was elucidated by UHPLC-MS using two groups of fruits at 28 d. RESULTS: After 25 d, the ABA concentration and polygalacturonase (PG) and beta-1,4-endoglucanase (EG) activities in ABA-treated fruits were significantly higher than those in untreated fruits. Large differences in the expression profiles were detected at 28 d. The expression of DEGs related to cell wall softening and ABA synthesis was largely triggered after 25 or 28 d. Sixty-nine differentially accumulated metabolites were ultimately annotated as related to fruit ripening. CONCLUSIONS: ABA stimulates blackberry fruit ripening by promoting cell wall enzyme activities, the expression of various ripening-related genes and metabolite accumulation.

A fruit-specific plasma membrane aquaporin subtype PIP1;1 is regulated during strawberry (Fragaria × ananassa) fruit ripening

2008 ◽  
Vol 132 (4) ◽  
pp. 538-551 ◽  
Author(s):  
Paula Mut ◽  
Claudia Bustamante ◽  
Gustavo Martínez ◽  
Karina Alleva ◽  
Moira Sutka ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Fruit Ripening ◽  
Fragaria Ananassa

Gene expression atlas of fruit ripening of octoploid strawberry (Fragaria×ananassa) and transcriptome assembly from RNA-seq data

2016 ◽  
Vol 33 ◽  
pp. S156
Author(s):  
José Federico Sánchez Sevilla ◽  
José G. Vallarino ◽  
Sonia Osorio ◽  
Aureliano Bombarely ◽  
Katharina Merchante ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruit Ripening ◽  
Transcriptome Assembly ◽  
Fragaria Ananassa ◽  
Rna Seq ◽  
Gene Expression Atlas ◽  
Expression Atlas

Quantitative proteomics and bioinformatic analysis provide new insight into protein function during avian eggshell biomineralization

2015 ◽  
Vol 113 ◽  
pp. 178-193 ◽  
Author(s):  
Pauline Marie ◽  
Valérie Labas ◽  
Aurélien Brionne ◽  
Grégoire Harichaux ◽  
Christelle Hennequet-Antier ◽  
...  
Keyword(s):  
Protein Function ◽  
Quantitative Proteomics ◽  
Bioinformatic Analysis ◽  
Insight Into

scholarly journals A FERONIA-Like Receptor Kinase Regulates Strawberry (Fragaria × ananassa) Fruit Ripening and Quality Formation

2017 ◽  
Vol 8 ◽  
Author(s):  
Meiru Jia ◽  
Ning Ding ◽  
Qing Zhang ◽  
Sinian Xing ◽  
Lingzhi Wei ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Receptor Kinase ◽  
Fragaria Ananassa ◽  
Quality Formation

scholarly journals Transcriptome profiling by RNA-Seq reveals differentially expressed genes related to fruit development and ripening characteristics in strawberry (Fragaria × ananassa)

2018 ◽  
Author(s):  
Panpan Hu ◽  
Gang Li ◽  
Xia Zhao ◽  
Fengli Zhao ◽  
Liangjie Li ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Fruit Ripening ◽  
Fruit Development ◽  
Transcriptome Profiling ◽  
Differentially Expressed ◽  
Fragaria Ananassa ◽  
Mads Box ◽  
Cell Wall Modification ◽  
Ripening Process ◽  
Fruit Development And Ripening

Strawberry (Fragaria × ananassa) is an ideal plant for fruit development and ripening research due to the rapid substantial changes in fruit color, aroma, taste and softening. To gain deeper insights into the genes that play a central regulatory role in strawberry fruit development and ripening characteristics, transcriptome profiling was performed for the large green fruit, white fruit, turning fruit, and red fruit stages of strawberry. A total of 6,608 differentially expressed genes (DEGs) with 2,643 up-regulated and 3,965 down-regulated genes were identified in the fruit development and ripening process. The DEGs related to fruit flavonoid biosynthesis, starch and sucrose biosynthesis, the citrate cycle, and cell-wall modification enzymes played important roles in the fruit development and ripening process. Particularly, some candidate genes related to the ubiquitin mediated proteolysis pathway and MADS-box were confirmed to be involved in fruit development and ripening according to their possible regulatory functions. Five ubiquitin-conjugating enzymes and ten MADS-box transcription factors were differentially expressed between the four fruit ripening stages. The expression levels of DEGs relating to color, aroma, taste, and softening of fruit were confirmed by quantitative real-time polymerase chain reaction. Our study provides important insights into the complicated regulatory mechanism underlying the fruit ripening characteristics in Fragaria × ananassa.

scholarly journals SRNAome and transcriptome analysis provide insight into strawberry fruit ripening

Genomics ◽  
2020 ◽  
Vol 112 (3) ◽  
pp. 2369-2378
Author(s):  
Yunxiang Wang ◽  
Wensheng Li ◽  
Hong Chang ◽  
Jiahua Zhou ◽  
Yunbo Luo ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening ◽  
Insight Into
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