scholarly journals Biotransformation of 4-methylcoumarins by cambial meristematic cells of Camptotheca acuminata

RSC Advances ◽  
2019 ◽  
Vol 9 (17) ◽  
pp. 9449-9456
Author(s):  
Yuhua Zhang ◽  
Jiayi Jiang ◽  
Ningbo Qin ◽  
Qian Zhang ◽  
Chunyan Yan
Keyword(s):  
Suspension Cultures ◽  
Meristematic Cell ◽  
Camptotheca Acuminata ◽  
Meristematic Cells ◽  
First Time ◽  
Biotransformation System

Cambial meristematic cell (CMC) suspension cultures were investigated as a new biotransformation system for the first time.

The subcellular concentration of ions and elements in thin cryosections of onion root meristem cells. An electron-probe EDS study

1984 ◽  
Vol 72 (1) ◽  
pp. 295-306
Author(s):  
I.L. Cameron ◽  
K.E. Hunter ◽  
N.K. Smith
Keyword(s):  
Electron Probe ◽  
Dry Weight ◽  
Cell Types ◽  
Meristematic Cells ◽  
Freeze Dried ◽  
Concentration Of Ions ◽  
Intracellular Compartments ◽  
Root Meristematic Cells ◽  
Storage Granules ◽  
First Time

Quantitative electron-probe energy dispersive X-ray microanalysis has, for the first time, been accomplished at a subcellular level in plant tissue using cryofixed and thin freeze-dried cryosections. The subcellular concentrations of Na+, Cl-, K+, P, S, Mg2+ and Ca2+ were measured in mol/kg dry weight in two types of root meristematic cells of the onion, Allium cepa. The cell wall of the meristematic cells had much higher concentrations of K+ and Ca2+ than was found in the intracellular compartments. Storage granules in the protoderm cells were about 6–12 times lower in P and were about four times higher in S as compared to other intracellular compartments. Comparison between the concentrations of ions and other elements in meristematic plant cells and in mouse cardiac myocytes confirms that major differences in cytoplasmic Na+ and Cl- concentrations do indeed exist between these cell types.

Major Indole Alkaloids Produced in Cell Suspension Cultures of Rhazya strict a Decaisne

1986 ◽  
Vol 41 (4) ◽  
pp. 385-390 ◽  
Author(s):  
Karl-Heinz Pawelka ◽  
Joachim Stöckigt
Keyword(s):  
Cell Suspension ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Structural Heterogeneity ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Minor Components ◽  
Rhazya Stricta ◽  
First Time

Eleven main alkaloids were identified from cell suspension cultures of Rhazya stricta grown in 4X-medium for 15 days. The alkaloids comprised the five groups Corynanthe, Strychnos, Eburnan, Secodine, and Aspidosperma and can be regarded as being typical Rhazya alkaloids, although the Strychnos alkaloid akuammicine has been isolated for the first time from the genus Rhazya. The most abundant compound was ( + )-1,2-dehydroaspidospermidine (15 mg/l medium) whereas all other constituents were synthesized in about 5 - 10 times lower amounts. More than 15 further alkaloids were formed as minor components which have not yet been identified. Cultivated Rhazya cells have been shown to be one of the richest alkaloid sources from apocynaceous cell suspensions. The isolated compounds, because of their structural heterogeneity, cannot be presently arranged into a scheme with coherent biosynthetic sequences.

scholarly journals Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

2020 ◽  
Vol 143 (3) ◽  
pp. 573-581
Author(s):  
Alexander Mehring ◽  
Janik Haffelder ◽  
Jonas Chodorski ◽  
Judith Stiefelmaier ◽  
Dorina Strieth ◽  
...  
Keyword(s):  
Ursolic Acid ◽  
Oleanolic Acid ◽  
Biomass Accumulation ◽  
Ocimum Basilicum ◽  
Meristematic Cells ◽  
Cultivation Period ◽  
Shake Flasks ◽  
Valuable Compounds ◽  
Whole Plants ◽  
First Time

AbstractThe application of plant suspension culture to produce valuable compounds, such as the triterpenoids oleanolic acid and ursolic acid, is a well-established alternative to the cultivation of whole plants. Cambial meristematic cells (CMCs) are a growing field of research, often showing superior cultivation properties compared to their dedifferentiated cell (DDC) counterparts. In this work, the first-time establishment of O. basilicum CMCs is demonstrated. DDCs and CMCs were cultivated in shake flasks and wave-mixed disposable bioreactors (wDBRs) and evaluated regarding triterpenoid productivity and biomass accumulation. CMCs showed characteristic small vacuoles and were found to be significantly smaller than DDCs. Productivities of oleanolic and ursolic acid of CMCs were determined at 3.02 ± 0.76 mg/(l*d) and 4.79 ± 0.48 mg/(l*d) after 19 days wDBR cultivation, respectively. These values were consistently higher than any productivities determined for DDCs over the observed cultivation period of 37 days. Elicitation with methyl jasmonate of DDCs and CMCs in shake flasks resulted in increased product contents up to 48 h after elicitor addition, with the highest increase found in CMCs at 232.30 ± 19.33% (oleanolic acid) and 192.44 ± 18.23% (ursolic acid) after 48 h.

scholarly journals Isolation and Identification of Camptothecin from Cells of Camptotheca acuminata Suspension Cultures

1974 ◽  
Vol 38 (1) ◽  
pp. 217-218 ◽  
Author(s):  
Kuniaki SAKATO ◽  
Haruo TANAKA ◽  
Noboru MUKAI ◽  
Masanaru MISAWA
Keyword(s):  
Suspension Cultures ◽  
Camptotheca Acuminata ◽  
Isolation And Identification

scholarly journals Regioselective hydroxylation and dehydrogenation of capsaicin and dihydrocapsaicin by cultured cells of Phytolacca americana

2021 ◽  
Vol 85 (1) ◽  
pp. 103-107
Author(s):  
Kei Shimoda ◽  
Tsubasa Ono ◽  
Hiroki Hamada
Keyword(s):  
Cultured Cells ◽  
Plant Cells ◽  
Suspension Cultures ◽  
The Other ◽  
Phytolacca Americana ◽  
Other Hand ◽  
Regioselective Hydroxylation ◽  
First Time

Abstract The biotransformations of capsaicin and dihydrocapsaicin were investigated using cultured plant cells of Phytolacca americana as biocatalysts. Four products, ie 15-hydroxycapsaicin, dihydrocapsaicin, 15-hydroxydihydrocapsaicin, and capsaicin 4-β-glucoside, were isolated from the suspension cultures of P. americana treated with capsaicin for 3 days, showing that capsaicin was regioselectively hydroxylated, reduced, and glucosylated by cultured P. americana cells. On the other hand, dihydrocapsaicin was regioselectively dehydrogenated, hydroxylated, reduced, and glucosylated to give four products, ie capsaicin, 15-hydroxycapsaicin, 15-hydroxydihydrocapsaicin, and capsaicin 4-β-glucoside, by cultured P. americana cells. In this paper, it is reported, for the first time, that dihydrocapsaicin is converted into 15-hydroxydihydrocapsaicin by plant cultured cells.

Influence de la température sur la floraison du Lis blanc

1978 ◽  
Vol 56 (1) ◽  
pp. 110-120 ◽  
Author(s):  
Simonne Rivierè
Keyword(s):  
Apical Meristem ◽  
The Other ◽  
Cold Period ◽  
Biological Cycle ◽  
Full Bloom ◽  
Meristematic Cells ◽  
Meristematic Activity ◽  
Combined Actions ◽  
Aerial Stem ◽  
First Time

The influence of thermoperiodic variations on the flowering of Lilium candidum is discussed here for the first time. Our experimentation was guided by our prior knowledge of the state of the apical meristem during the biological cycle. We concluded that forcing, to be effective, must occur on growing bulbs when the meristem of the lily is in the intermediate phase.We analyzed whether a cold period was necessary. When lily plants were cultivated for 8 days at 2 °C, the meristem reached the prefloral state at the beginning of the warm period (15 °C) which followed. The inflorescence is then in full bloom during the hot period (22 °C). Flowering occurs at the end of February.However, if the cold period is eliminated (with no change in the other periods), the terminal meristem loses its activity and differentiates. It is replaced by a small cluster of meristematic cells. A bulb develops at the top of the stem replacing the inflorescence. In this case, meristematic activity now begins at the axil of the leaves and the aerial stem. The axillary buds thus produced are a result of the combined actions of suppression of apical dominance after the loss of terminal meristem activity and the application of hot temperatures (22 °C).

scholarly journals Anthocyanic vacuolar inclusions in cell suspension cultures of Camptotheca acuminata Decne

Caryologia ◽  
2007 ◽  
Vol 60 (1-2) ◽  
pp. 165-168
Author(s):  
Valletta Alessio ◽  
Isabella Moro ◽  
Nicoletta Rascio ◽  
Gabriella Pasqua
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Camptotheca Acuminata

scholarly journals Karyotype evolution of the genus Eranthis Salisb. (Ranunculaceae)

2020 ◽  
Vol 19 (2) ◽  
pp. 056-060
Author(s):  
E. Yu Mitrenina ◽  
A. S. Erst
Keyword(s):  
Chromosome Number ◽  
Comparative Study ◽  
Karyotype Evolution ◽  
Basic Chromosome Number ◽  
B Chromosomes ◽  
Basic Chromosome ◽  
Meristematic Cells ◽  
Pericentric Inversions ◽  
Root Meristematic Cells ◽  
First Time

We have conducted comparative study of karyotypes for nine Eranthis Salisb. species: E. bulgarica (Stef.)Stef., E. hyemalis (L.) Salisb., E. longistipitata Regel (section Eranthis), E. byunsanensis B. Y. Sun, E. lobulata W. T.Wang, E. pinnatifida Maxim., E. sibirica DC., E. stellata Maxim., and E. tanhoensis Erst (section Shibateranthis). Thespecies-specifity of karyotypes was established for all species investigated. The chromosomes of each species weremedium or large in size (4–12 µm). Besides E. sibirica and E. tanhoensis, all the investigated specimens had diploidcytotypes with 2n = 16 and the basic chromosome number x = 8. Plants from five E. sibirica populations were tetraploidand hexaploid with x = 7, 2n = 28 and 2n = 42 respectively. Plants from seven E. tanhoensis populations were diploid withx = 7 and 2n = 14. Diploid karyotypes of Eranthis included 4–5 pairs of large equal-armed (metacentric) chromosomes,and 2–4 pairs of unequal-armed chromosomes belonging to different morphological types (submetacentric, subtelocentric,and acrocentric ones). We have revealed B chromosomes in root meristematic cells of E. lobulata and E. tanhoensis forthe first time. We suppose that the key developments in Eranthis karyotype`s evolution were pericentric inversions,polyploidy, and probably translocations.

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