scholarly journals A highly sensitive and selective fluorescent probe for quantitative detection of Al3+ in food, water, and living cells

RSC Advances ◽  
2019 ◽  
Vol 9 (18) ◽  
pp. 10414-10419 ◽  
Author(s):  
Qian Jiang ◽  
Mingxin Li ◽  
Jie Song ◽  
Yiqin Yang ◽  
Xu Xu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
High Sensitivity ◽  
Living Cells ◽  
Food Samples ◽  
Quantitative Detection ◽  
Lower Detection Limit ◽  
Highly Sensitive ◽  
Lower Detection ◽  
Wide Ph Range ◽  
Ph Range

Probe 2a exhibiting high sensitivity, good selectivity, wide pH range, lower detection limit, and rapid detection for Al3+, probe 2a was applied for the successful detection of Al3+ in water samples, food samples and HeLa cells.

Rapid Detection and Quantification of Lanthanide Ions Using High-Speed Polymerase Chain Reactions

2014 ◽  
Vol 875-877 ◽  
pp. 749-754
Author(s):  
Asuka Kikuchi ◽  
Takashi Kadono ◽  
Kazuhiro Hashimoto ◽  
Kazuya Uezu ◽  
Tomonori Kawano
Keyword(s):  
Rapid Detection ◽  
High Speed ◽  
High Sensitivity ◽  
Lanthanide Ions ◽  
Quantitative Detection ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Novel Approach ◽  
Highly Sensitive ◽  
Polymerase Chain

Recently, a novel approach to a highly sensitive and quantitative detection of rare earth element (REE) ions including La3+, Eu3+ and Tb3+, by the polymerase chain reaction (PCR) technique, has been reported. The detection of REE ions is based on the catalytic nature of REE ions targeting the deoxyribonucleic acid (DNA), thus monitoring of the ions can be achieved by reading the level of intact DNA by PCR. Despite of its high sensitivity (at ppb to ppt levels), the conventional PCR-based REE detection protocol requires certain length of time (1-2 hours). In the present study, we modified the PCR-based REE detection protocols by employing the high-speed PCR, and performed the automated and rapid detection of La3+ in small-sized aqueous samples within 5min.

Rapid, highly sensitive and quantitative detection of interleukin 6 based on SERS magnetic immunoassay

2021 ◽  
Vol 13 (15) ◽  
pp. 1823-1831
Author(s):  
Xiaomei Wang ◽  
Li Ma ◽  
Shijiao Sun ◽  
Tingwei Liu ◽  
Hao Zhou ◽  
...  
Keyword(s):  
Interleukin 6 ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Detection Time ◽  
Highly Sensitive ◽  
Sandwich Method

We have developed a SERS magnetic immunoassay method based on the principle of sandwich method for rapid and quantitative detection of IL-6. The developed SERS method has the advantages of high sensitivity and detection time is only 15 min.

scholarly journals Rapid Method Using Two Microbial Enzymes for Detection of l-Abrine in Food as a Marker for the Toxic Protein Abrin

2014 ◽  
Vol 81 (5) ◽  
pp. 1610-1615 ◽  
Author(s):  
Anthony G. Dodge ◽  
Kelvin Carrasquillo ◽  
Luis Rivera ◽  
Lei Xu ◽  
Lawrence P. Wackett ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Visual Detection ◽  
Food Samples ◽  
Content Type ◽  
Toxic Protein ◽  
Beverage Samples ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Complex Sample ◽  
Food And Beverage

ABSTRACTAbrin is a toxic protein produced by the ornamental plantAbrus precatorius, and it is of concern as a biothreat agent. The small coextracting moleculeN-methyl-l-tryptophan (l-abrine) is specific to members of the genusAbrusand thus can be used as a marker for the presence or ingestion of abrin. Current methods for the detection of abrin orl-abrine in foods and other matrices require complex sample preparation and expensive instrumentation. To develop a fast and portable method for the detection ofl-abrine in beverages and foods, theEscherichia coliproteinsN-methyltryptophan oxidase (MTOX) and tryptophanase were expressed and purified. The two enzymes jointly degradedl-abrine to products that included ammonia and indole, and colorimetric assays for the detection of those analytes in beverage and food samples were evaluated. An indole assay using a modified version of Ehrlich's/Kovac's reagent was more sensitive and less subject to negative interferences from components in the samples than the Berthelot ammonia assay. The two enzymes were added into food and beverage samples spiked withl-abrine, and indole was detected as a degradation product, with the visual lower detection limit being 2.5 to 10.0 μM (∼0.6 to 2.2 ppm)l-abrine in the samples tested. Results could be obtained in as little as 15 min. Sample preparation was limited to pH adjustment of some samples. Visual detection was found to be about as sensitive as detection with a spectrophotometer, especially in milk-based matrices.

Glucose Biosensor Based on Glucose Oxidase-Horseradish Peroxidase/Multiporous Tin Oxide (SnO2) Modified Electrode

2021 ◽  
Vol 21 (5) ◽  
pp. 3059-3064
Author(s):  
A. K. M. Kafi ◽  
Aizam Bin Kasri ◽  
Rajan Jose
Keyword(s):  
Horseradish Peroxidase ◽  
Glucose Oxidase ◽  
Tin Oxide ◽  
Electrode Potential ◽  
High Sensitivity ◽  
Glucose Biosensor ◽  
Glucose Detection ◽  
Lower Detection Limit ◽  
Vis Spectroscopy ◽  
Lower Detection

The fabrication of a network of glucose oxidase-horseradish peroxidase/tin oxide (GOx-HRP/SnO2), immobilized onto a glassy carbon electrode (GCE) and its utilization as a biosensor for glucose detection is reported. The network established with GOx-HRP/SnO2 possess high sensitivity and stability by performing the electrocatalytic features in the sensing of glucose. The turbidity of fabrication had been scanned and analyzed using UV-vis spectroscopy. The morphology and composition of the fabricated GOx-HRP/SnO2 networks were characterized by scanning electron microscopy (SEM). Cyclic voltammetry and amperometry were employed to study the electrochemical properties of the proposed biosensor. The effect of applied electrode potential and pH were systemically investigated. The biosensor responds to glucose at work potential values between −400 mV, and exhibited a lower detection limit (0.025 mM) and long linear range (0.25 to 7.0 mM), and was resistant to common interferences.

scholarly journals Determination of epinephrine by the Briggs-Rauscher oscillating system using non-equilibrium stationary state

2012 ◽  
Vol 77 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Jinzhang Gao ◽  
Yanjun Liu ◽  
Jie Ren ◽  
Xiaoli Zhang ◽  
Li Ming ◽  
...  
Keyword(s):  
Detection Limit ◽  
Stationary State ◽  
Lower Detection Limit ◽  
Highly Sensitive ◽  
Lower Detection ◽  
Oscillating Reaction ◽  
The Difference ◽  
Foreign Species ◽  
Non Equilibrium

A highly sensitive method for the determination of epinephrine was proposed, which was based on the perturbation of epinephrine to Briggs-Rauscher oscillating system involving malonic acid, Mn2+, H+, IO3 - and H2O2 at non-equilibrium stationary state. The concentration of KIO3 was chosen as a control parameter to find the bifurcation point in this paper. Results showed that a well linear relationship between the difference of potential and the negative logarithm concentrations of epinephrine existed in the range of 1.1?10-7?5.2?10-9 mol L-1 with a lower detection limit of 6.8?10-10mol L-1 and a correlation coefficient of 0.9974. Compared to the classical oscillating reaction, this method has a lower detection limit and wider linear range. The effects of some foreign species, which may possibly be existed with epinephrine, on determination were also investigated. The proposed method has been successfully used to determine the epinephrine both in the serum and adrenaline hydrochloride injection.

An Ultrasensitive Immunosensor for the Detection of Carcinoembryonic Antigens Utilizing a Nb-Doped Titanium Dioxide Nanocomposite Film

NANO ◽  
2015 ◽  
Vol 10 (04) ◽  
pp. 1550060 ◽  
Author(s):  
He Li ◽  
Junli Lu ◽  
Yu Wang ◽  
Fenyun Yi ◽  
Xindong Guo
Keyword(s):  
Titanium Dioxide ◽  
Influence Factors ◽  
High Sensitivity ◽  
Electrochemical Immunosensor ◽  
Optimal Conditions ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Electrochemical Signal ◽  
Doped Titanium Dioxide ◽  
Excellent Selectivity

An ultrasensitive electrochemical immunosensor based on Nb-doped titanium dioxide was developed for detection of carcinoembryonic antigen (CEA). The electrochemical signal enhancement was due to the synergistic effect by NbT and nanogold. The influence factors (pH, temperature and incubation time) were investigated in detail. Under optimal conditions, the immunosensor was able to detect the concentration of CEA in the range of 0.5 ng ⋅ mL-1 to 150 ng ⋅ mL-1, with a lower detection limit of 8.95 pg ⋅ mL-1. The proposed immunosensor showed high sensitivity, excellent selectivity and good stability.

scholarly journals An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells

ChemistryOpen ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1251-1257
Author(s):  
Yupeng Liu ◽  
Tian‐Bing Ren ◽  
Dan Cheng ◽  
Jianing Hou ◽  
Dongdong Su ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Rapid Detection ◽  
Living Cells ◽  
Highly Sensitive ◽  
Ratiometric Fluorescent Probe

scholarly journals Correction: A highly sensitive and selective fluorescent probe for quantitative detection of Al3+ in food, water, and living cells

RSC Advances ◽  
2019 ◽  
Vol 9 (30) ◽  
pp. 16966-16966
Author(s):  
Qian Jiang ◽  
Mingxin Li ◽  
Jie Song ◽  
Yiqin Yang ◽  
Xu Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Quantitative Detection ◽  
Highly Sensitive

Correction for ‘A highly sensitive and selective fluorescent probe for quantitative detection of Al3+ in food, water, and living cells’ by Qian Jiang et al., RSC Adv., 2019, 9, 10414–10419.

scholarly journals A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4768 ◽  
Author(s):  
Zongcheng Wang ◽  
Huihuang Zheng ◽  
Chengliang Zhang ◽  
Dongfang Tang ◽  
Qiyao Wu ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Quantitative Detection ◽  
Fast Reaction ◽  
Detection Range ◽  
Long Wavelength ◽  
Turn On

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

Highly Selective Caffeine Coated-Wire Electrode Based on the Molecularly Imprinted Polymer

2012 ◽  
Vol 554-556 ◽  
pp. 369-373
Author(s):  
Xiao Jun Guo ◽  
Shou Lian Wei ◽  
Yong Liu
Keyword(s):  
Wire Electrode ◽  
Imprinted Polymer ◽  
Selective Electrode ◽  
Molecularly Imprinted ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Coated Wire ◽  
Ph Range ◽  
Coated Wire Electrode

The molecular imprinted polymer (MIP) was synthesized by precipitation polymerization with caffeine as template, methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. A selective electrode for the potentiometric determination of caffeine in the aqueous medium was developed using the MIP-modified coated-wire electrode. The electrode shows response for caffeine in a concentration range of 1.0×10-8 to 1.0×10-5 M with a lower detection limit of 5.0×10-9 M. The electrode can be used for more than 100 times. The response time is 15 s and the signal is constant in the pH range 6.4 to 9.0. The electrode can be successfully applied for the monitoring of caffeine in syrup sample.

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