scholarly journals High aldehyde dehydrogenase activity does not protect colon cancer cells against TPCS2a-sensitized photokilling

2020 ◽  
Vol 19 (3) ◽  
pp. 308-312
Author(s):  
Judith Jing Wen Wong ◽  
Pål Kristian Selbo
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Aldehyde Dehydrogenase ◽  
Therapy Resistance ◽  
Colon Cancer Cells ◽  
Aldh Activity ◽  
Aldehyde Dehydrogenases ◽  
Aldehyde Dehydrogenase Activity

Overexpression of Aldehyde Dehydrogenases (ALDH) has been linked to cancer therapy resistance. Here we show that high ALDH activity do not cause resistance against photodynamic therapy using the PCI-photosensitizer TPCS2a/fimaporfin.

Photodynamic Therapy at Low-Light Fluence Rate: in vitro Assays on Colon Cancer Cells

2019 ◽  
Vol 25 (1) ◽  
pp. 1-6 ◽  
Author(s):  
J. A. Rodrigues ◽  
R. Amorim ◽  
M. F. Silva ◽  
F. Baltazar ◽  
R. F. Wolffenbuttel ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Fluence Rate ◽  
Colon Cancer Cells ◽  
Low Light ◽  
Light Fluence

scholarly journals Effective Photodynamic Therapy for Colon Cancer Cells Using Chlorin e6 Coated Hyaluronic Acid-Based Carbon Nanotubes

2020 ◽  
Vol 21 (13) ◽  
pp. 4745 ◽  
Author(s):  
Prabhavathi Sundaram ◽  
Heidi Abrahamse
Keyword(s):  
Carbon Nanotubes ◽  
Colon Cancer ◽  
Cell Death ◽  
Photodynamic Therapy ◽  
Hyaluronic Acid ◽  
Cancer Cells ◽  
Chlorin E6 ◽  
Therapeutic Modality ◽  
Size Analysis ◽  
Colon Cancer Cells

Colon cancer is the third major cancer contributor to mortality worldwide. Nanosized particles have attracted attention due to their possible contribution towards cancer treatment and diagnosis. Photodynamic therapy (PDT) is a cancer therapeutic modality that involves a light source, a photosensitizer and reactive oxygen species. Carbon nanotubes are fascinating nanocarriers for drug delivery, cancer diagnosis and numerous potential applications due to their unique physicochemical properties. In this study, single walled carbon nanotubes (SWCNTs) were coupled with hyaluronic acid (HA) and chlorin e6 (Ce6) coated on the walls of SWCNTs. The newly synthesized nanobiocomposite was characterized using ultraviolet-visible spectroscopy, Fourier transform electron microscopy (FTIR), X-ray diffraction analysis (XRD), particle size analysis and zeta potential. The loading efficiency of the SWCNTs-HA for Ce6 was calculated. The toxicity of the nanobiocomposite was tested on colon cancer cells using PDT at a fluence of 5 J/cm2 and 10 J/cm2. After 24 h, cellular changes were observed via microscopy, LDH cytotoxicity assay and cell death induction using annexin propidium iodide. The results showed that the newly synthesized nanobiocomposite enhanced the ability of PDT to be a photosensitizer carrier and induced cell death in colon cancer cells.

scholarly journals Photodynamic Therapy Using Endoscopy Capsule Robot

2020 ◽  
pp. 93-100
Author(s):  
Sherif Hamdy ElGohary ◽  
Yomna Sabah Mohamed ◽  
Mennatallah Hany Elkhodary ◽  
Omnya Ahmed ◽  
Mennatallah Hesham
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Digestive System ◽  
Colon Cancer Cells ◽  
Effective Wavelength ◽  
Electromagnetic Actuation ◽  
Led Light ◽  
Actuation System ◽  
Electromagnetic Actuation System

Among the photosensitizers used in Photodynamic therapy (PDT) technique for cancer treatment, it is found out that the Methylene blue and glycoconjugates chlorine are the best ones for this purpose. In this paper, it is suggested to use Active Capsule Wireless Endoscopy Robot instead of the traditional endoscope. The capsule has many valuable features. It uses LEDs as a source of light in the PDT to kill the colon cancer cells. So, the doctor can make use of the advantage of applying the LED light locally at the tumor which was previously injected by the photosensitizers, the light activates these photosensitizers and a photochemical reaction starts that makes the colon cancer cells die. The light with effective wavelength and power density, energy level and controlled LED light intensity will be applied. Active locomotion capsule endoscopy with an electromagnetic actuation system that can achieve a 3-D locomotion and guidance within the digestive system. The paper also discussed how to manage the required power in the capsule for all parts, LEDs, camera, transceiver, and locomotion.

The influence of hypericin-mediated photodynamic therapy on secretion of selected interleukins by colon cancer cells in vitro

2019 ◽  
Author(s):  
Aleksandra Kawczyk-Krupka ◽  
Marta Kaleta-Richter ◽  
Anna Miedzybrodzka ◽  
Wojciech Latos ◽  
Aleksander Sieron ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Colon Cancer Cells

scholarly journals The Influence of Hypericin-Mediated Photodynamic Therapy on Interleukin-8 and -10 Secretion in Colon Cancer Cells

2020 ◽  
Vol 19 ◽  
pp. 153473542091893 ◽  
Author(s):  
Marta Kaleta-Richter ◽  
David Aebisher ◽  
Dagmara Jaworska ◽  
Zenon Czuba ◽  
Grzegorz Cieślar ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Cell Line ◽  
Sw480 Cell ◽  
Experimental Models ◽  
Colon Cancer Cells ◽  
Sw620 Cell ◽  
Sublethal Doses

The aim of this study was to measure the secretion of interleukin (IL)-8 and -10 during an elicited immune response following sublethal doses of hypericin-mediated photodynamic therapy (HY-PDT) in experimental models of residual colon cancer cells in vitro. Investigations were performed on the cancer cell lines SW480 and SW620. Each cell line was exposed to 3 different concentrations of the photosensitizer HY and various doses of irradiation. The cell metabolic activity using an MTT assay was performed and then the measurement of IL-8 and IL-10 secretion was achieved using the Bio-Plex ProTMAssay. There was a statistically significant amplification of IL-8 secretion during HY-PDT in the SW620 cell line (at 1 J/cm2: P = .01, 5 J/cm2: P = .002, and 10 J/cm2: P = .025) and a statistically significant decrease in IL-8 during HY-PDT in the SW480 cell line (at 1 J/cm2: P = .05, 5 J/cm2: P = .035, and 10 J/cm2: P = .035). No statistically significant differences in IL-10 concentration were found following HY-PDT in the SW480 (at 1 J/cm2: P > .4, 5 J/cm2: P = .1, and 10 J/cm2: P = .075) or in the SW620 cell line (at 1 J/cm2: P > .4, 5 J/cm2: P > .4, and 10 J/cm2: P > .4). HY-PDT can both eliminate and control a primary tumor via cytotoxic effects, and at sublethal doses, it can affect IL release by colon cancer cells. In this experiment, this influence depended on the level of tumor cell metastatic activity.

scholarly journals Knockdown of Parkinson’s disease-related gene ATP13A2 reduces tumorigenesis via blocking autophagic flux in colon cancer

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Qian Chen ◽  
Li Zhong ◽  
Chao Zhou ◽  
Yan Feng ◽  
Quan-xing Liu ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Colon Cancer ◽  
Parkinson's Disease ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Cancer Prognosis ◽  
Autophagic Flux ◽  
Colon Cancer Cells ◽  
Related Gene ◽  
Disease Related Gene

Abstract Background Accumulating evidence shows that Parkinson’s disease is negatively associated with colon cancer risk, indicating that Parkinson’s disease family proteins may be involved in the initiation of colon cancer. Here, we aimed to identify a Parkinson’s disease-related gene involved in colon cancer, elucidate the underlying mechanisms, and test whether it can be used as a target for cancer therapy. Methods We first screened colon cancer and normal tissues for differential expression of Parkinson’s disease-associated genes and identified ATP13A2, which encodes cation-transporting ATPase 13A2, as a putative marker for colon cancer. We next correlated ATP13A2 expression with colon cancer prognosis. We performed a series of ATP13A2 knockdown and overexpression studies in vitro to identify the contribution of ATP13A2 in the stemness and invasive capacity of colon cancer cells. Additionally, autophagy flux assay were determined to explore the mechanism of ATP13A2 induced stemness. Finally, we knocked down ATP13A2 in mice using siRNA to determine whether it can be used as target for colon cancer treatment. Results Colon cancer patients with high ATP13A2 expression exhibit shorter overall survival than those with low ATP13A2. Functionally, ATP13A2 acts as a novel stimulator of stem-like traits. Furthermore, knockdown of ATP13A2 in HCT116 resulted in decreased levels of cellular autophagy. Additionally, bafilomycin A1, an autophagy inhibitor, reversed the ATP13A2-induced stemness of colon cancer cells. Lastly treatment with ATP13A2 siRNA reduced the volume of colon cancer xenografts in mice. Conclusions The PD-associated gene ATP13A2 is involved in colon cancer stemness through regulation of autophagy. Furthermore, ATP13A2 is a novel prognostic biomarker for colon cancer and is a potential target for colon cancer therapy.

The role of p53 in the efficiency of photodynamic therapy with hypericin and subsequent long-term survival of colon cancer cells

2009 ◽  
Vol 8 (11) ◽  
pp. 1558 ◽  
Author(s):  
Jaromír Mikeš ◽  
Ján Koval' ◽  
Rastislav Jendželovský ◽  
Veronika Sačková ◽  
Ivana Uhrinová ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Term Survival ◽  
Long Term Survival

Influence of ALA-mediated photodynamic therapy on secretion of interleukins 6, 8 and 10 by colon cancer cells in vitro

2018 ◽  
Vol 22 ◽  
pp. 137-139 ◽  
Author(s):  
Aleksandra Kawczyk-Krupka ◽  
Zenon Czuba ◽  
Wojciech Latos ◽  
Katarzyna Wasilewska ◽  
Thomas Verwanger ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Colon Cancer Cells

MDM-2 Overexpression in Human Stem-Like and Mature Colon Cancer Cells as a Target for Anti-Cancer Therapy: Steps toward a Translational Model

2015 ◽  
Vol 221 (4) ◽  
pp. S142
Author(s):  
Mohammad F. Shaikh ◽  
Blake D. Babcock ◽  
Elizabeth Gleeson ◽  
Patrice Love ◽  
Katlin Davitt ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Translational Model ◽  
Anti Cancer

scholarly journals Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy using light-emitting diodes in human colon cancer cells

2013 ◽  
Vol 29 (3) ◽  
pp. 911-916 ◽  
Author(s):  
TOMOYA HATAKEYAMA ◽  
YASUTOSHI MURAYAMA ◽  
SHUHEI KOMATSU ◽  
ATSUSHI SHIOZAKI ◽  
YOSHIAKI KURIU ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Photodynamic Therapy ◽  
Cancer Cells ◽  
Light Emitting Diodes ◽  
Aminolevulinic Acid ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Light Emitting ◽  
Human Colon Cancer Cells
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