scholarly journals Highly selective staining and quantification of intracellular lipid droplets with a compact push–pull fluorophore based on benzothiadiazole

2020 ◽  
Vol 18 (3) ◽  
pp. 495-499 ◽  
Author(s):  
S. Israel Suarez ◽  
Caroline C. Warner ◽  
Heather Brown-Harding ◽  
Andrea M. Thooft ◽  
Brett VanVeller ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Human Cells ◽  
Intracellular Lipid ◽  
Selective Staining ◽  
Fluorescent Stain

A robust lipophilic dye, based on the structures of the benzothiadiazole heterocycle, was shown to be a potent fluorescent stain for the selective imaging of lipid droplets (LDs) within both live and fixed human cells.

scholarly journals Selective Staining and Quantification of Intracellular Lipid Droplets with CBD-Fluor, a New and Versatile Push-Pull Fluorophore

2019 ◽  
Author(s):  
S. Israel Suarez ◽  
Caroline C. Warner ◽  
Heather Brown-Harding ◽  
andrea thooft ◽  
Brett VanVeller ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Stokes Shift ◽  
Nile Red ◽  
Live Cells ◽  
Intracellular Lipid ◽  
Selective Staining ◽  
Large Stokes Shift ◽  
Fluorescent Stain ◽  
Photo Stability

A novel lipophilic dye, based on the structures of benzothiadiazole heterocycles, was shown to be an effective fluorescent stain for the imaging of lipid droplets (LDs) within both fixed and live cells. Its high photo stability, large Stokes shift, and improved staining and specificity over that of commercial dye Nile Red highlight its great potential as a versatile chemical tool for facilitating LD imaging and research.

scholarly journals Selective Staining and Quantification of Intracellular Lipid Droplets with CBD-Fluor, a New and Versatile Push-Pull Fluorophore

2019 ◽  
Author(s):  
S. Israel Suarez ◽  
Caroline C. Warner ◽  
Heather Brown-Harding ◽  
andrea thooft ◽  
Brett VanVeller ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Stokes Shift ◽  
Nile Red ◽  
Live Cells ◽  
Intracellular Lipid ◽  
Selective Staining ◽  
Large Stokes Shift ◽  
Fluorescent Stain ◽  
Photo Stability

A novel lipophilic dye, based on the structures of benzothiadiazole heterocycles, was shown to be an effective fluorescent stain for the imaging of lipid droplets (LDs) within both fixed and live cells. Its high photo stability, large Stokes shift, and improved staining and specificity over that of commercial dye Nile Red highlight its great potential as a versatile chemical tool for facilitating LD imaging and research.

New Fluoranthene FLUN-550 as a Fluorescent Probe for Selective Staining and Quantification of Intracellular Lipid Droplets

2014 ◽  
Vol 16 (3) ◽  
pp. 756-759 ◽  
Author(s):  
Atul Goel ◽  
Ashutosh Sharma ◽  
Manoj Kathuria ◽  
Arindam Bhattacharjee ◽  
Ashwni Verma ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Lipid Droplets ◽  
Intracellular Lipid ◽  
Selective Staining

A new type of biocompatible fluorescent probe AFN for fixed and live cell imaging of intracellular lipid droplets

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Ashutosh Sharma ◽  
Shahida Umar ◽  
Parmita Kar ◽  
Kavita Singh ◽  
Monika Sachdev ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Live Cell Imaging ◽  
Lipid Droplets ◽  
Cell Imaging ◽  
Intracellular Lipid ◽  
Selective Staining ◽  
Cervical Cancer Cells ◽  
New Type ◽  
Human Cervical Cancer Cells ◽  
Human Cervical Cancer

We discovered a new type of nontoxic, highly fluorogenic and biocompatible probe AFN for selective staining of intracellular Lipid Droplets (LDs) in both fixed and live human cervical cancer cells (HeLa) and 3T3-L1 pre-adipocytes without any background artifacts.

A Fused Thiophene-S,S-Dioxide-Based Super-Photostable Fluorescent Marker for Lipid Droplets

2020 ◽  
Author(s):  
Masayasu Taki ◽  
Keiji Kajiwara ◽  
Eriko Yamaguchi ◽  
Yoshikatsu Sato ◽  
Shigehiro Yamaguchi
Keyword(s):  
Small Molecules ◽  
Lipid Droplets ◽  
Trajectory Analysis ◽  
Super Resolution ◽  
Fluorescent Marker ◽  
Selective Staining ◽  
Fluorescent Markers ◽  
Biological Studies ◽  
Particle Level

Lipid droplets (LDs) are essential organelle in most eukaryotes, and tracking intracellular LDs dynamics using synthetic small molecules is crucial for biological studies. However, only a limited number of fluorescent markers that satisfy all requirements, such as the selective staining of LDs, high photostability, and sufficient biocompatibility, have been developed. Herein, we report a series of donor-p-acceptor dyes based on the thiophene-containing fused polycyclic scaffold [1]benzothieno[3,2-<i>b</i>][1]benzothiophene (BTBT), in which either or both thiophene rings are oxidized into thiophene-<i>S</i>,<i>S</i>-dioxide to form an electron-accepting building block. Among these dyes, LAQ1 satisfied all the aforementioned requirements, and allowed us capturing ultra-small LDs on the endoplasmic reticulum (ER) membrane by stimulation emission depletion (STED) microscopy with a super-resolution below the diffraction limit of light. Moreover, the extremely high photostability of LAQ1 enabled recording the lipolysis of LDs and the concomitant lipogenesis as well as long-term trajectory analysis of micro LDs at the single particle level in living cells.

scholarly journals RNA decay in processing bodies is indispensable for adipogenesis

2021 ◽  
Vol 12 (4) ◽  
Author(s):  
Ryotaro Maeda ◽  
Daisuke Kami ◽  
Akira Shikuma ◽  
Yosuke Suzuki ◽  
Toshihiko Taya ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Adipogenic Differentiation ◽  
Negative Regulator ◽  
Translational Repression ◽  
Rna Decay ◽  
Significant Suppression ◽  
Intracellular Lipid ◽  
Processing Bodies ◽  
Binding Partner ◽  
The Stability

AbstractThe RNA decay pathway plays key regulatory roles in cell identities and differentiation processes. Although adipogenesis is transcriptionally and epigenetically regulated and has been thoroughly investigated, how RNA metabolism that contributes to the stability of phenotype-shaping transcriptomes participates in differentiation remains elusive. In this study, we investigated Ddx6, an essential component of processing bodies (PBs) that executes RNA decay and translational repression in the cytoplasm and participates in the cellular transition of reprogramming. Upon adipogenic induction, Ddx6 dynamically accumulated to form PBs with a binding partner, 4E-T, at the early phase prior to emergence of intracellular lipid droplets. In contrast, preadipocytes with Ddx6 knockout (KO) or 4E-T knockdown (KD) failed to generate PBs, resulting in significant suppression of adipogenesis. Transcription factors related to preadipocytes and negative regulators of adipogenesis that were not expressed under adipogenic stimulation were maintained in Ddx6-KO and 4E-T-KD preadipocytes under adipogenic induction. Elimination of Dlk1, a major negative regulator of adipogenesis, in 3T3L1 Ddx6-KO cells did not restore adipogenic differentiation capacity to any extent. Similar to murine cells, human primary mesenchymal stem cells, which can differentiate into adipocytes upon stimulation with adipogenic cocktails, required DDX6 to maturate into adipocytes. Therefore, RNA decay of the entire parental transcriptome, rather than removal of a strong negative regulator, could be indispensable for adipogenesis.

scholarly journals Association between Endoscopic Milk-White Mucosa, Epithelial Intracellular Lipid Droplets, and Histological Grade of Superficial Non-Ampullary Duodenal Epithelial Tumors

Diagnostics ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 769
Author(s):  
Yuko Hara ◽  
Kenichi Goda ◽  
Shinichi Hirooka ◽  
Takehiro Mitsuishi ◽  
Masahiro Ikegami ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Histological Grade ◽  
Rank Correlation ◽  
Intraepithelial Neoplasia ◽  
Low Grade ◽  
Intracellular Lipid ◽  
En Bloc ◽  
Spearman’S Rank Correlation ◽  
Spearman’S Rank Correlation Coefficient ◽  
Positive Rate

We previously reported that superficial non-ampullary duodenal tumors (SNADETs) commonly had a whitish mucosal surface, named milk-white mucosa (MWM). The aim of this study was to evaluate the association of MWM with epithelial intracellular lipid droplets (immunohistochemically stained by adipose differentiation-related protein (ADRP)) and histological tumor grades. We reviewed endoscopic images and the histopathology of SNADETs resected en bloc endoscopically. We analyzed the correlation between the positive rates of endoscopic MWM in preoperative endoscopy and resected specimens, and ADRP-positive rates in the resected specimens. Associations between the MWM-positive rates and tumor grades, high-grade intraepithelial neoplasia (HGIN)/intramucosal carcinoma (IC), and low-grade intraepithelial neoplasia (LGIN) were analyzed. All the 92 SNADETs analyzed were <20 mm and histologically classified into 39 HGIN/IC and 53 LGIN. Spearman’s rank correlation coefficient showed a significant correlation between MWM-positive and ADRP-positive rates (p < 0.001). MWM-positive rates were significantly lower in the HGIN/IC than in the LGIN in preoperative endoscopy (p < 0.001) and resected specimens (p = 0.02). Our results suggest that endoscopic MWM is closely associated with epithelial intracellular lipid droplets and that the MWM-positive rate may be a predictor of histological grade in small SNADETs.

scholarly journals Specific Imaging of Intracellular Lipid Droplets Using a Benzothiadiazole Derivative with Solvatochromic Properties

2017 ◽  
Vol 28 (5) ◽  
pp. 1363-1370 ◽  
Author(s):  
Hanna Appelqvist ◽  
Kati Stranius ◽  
Karl Börjesson ◽  
K. Peter. R. Nilsson ◽  
Christine Dyrager
Keyword(s):  
Lipid Droplets ◽  
Intracellular Lipid

scholarly journals Seipin is required for converting nascent to mature lipid droplets

eLife ◽  
2016 ◽  
Vol 5 ◽  
Author(s):  
Huajin Wang ◽  
Michel Becuwe ◽  
Benjamin E Housden ◽  
Chandramohan Chitraju ◽  
Ashley J Porras ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Lipid Synthesis ◽  
Human Cells ◽  
Lipid Transfer ◽  
Cellular Lipid ◽  
Contact Sites ◽  
Discrete Step

How proteins control the biogenesis of cellular lipid droplets (LDs) is poorly understood. Using Drosophila and human cells, we show here that seipin, an ER protein implicated in LD biology, mediates a discrete step in LD formation—the conversion of small, nascent LDs to larger, mature LDs. Seipin forms discrete and dynamic foci in the ER that interact with nascent LDs to enable their growth. In the absence of seipin, numerous small, nascent LDs accumulate near the ER and most often fail to grow. Those that do grow prematurely acquire lipid synthesis enzymes and undergo expansion, eventually leading to the giant LDs characteristic of seipin deficiency. Our studies identify a discrete step of LD formation, namely the conversion of nascent LDs to mature LDs, and define a molecular role for seipin in this process, most likely by acting at ER-LD contact sites to enable lipid transfer to nascent LDs.

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