Fine structural tuning of styryl-based dyes for fluorescence and CD-based sensing of various ds-DNA/RNA sequences

2019 ◽  
Vol 17 (35) ◽  
pp. 8243-8258 ◽  
Author(s):  
Valentina Botti ◽  
Alessio Cesaretti ◽  
Željka Ban ◽  
Ivo Crnolatac ◽  
Giuseppe Consiglio ◽  
...  
Keyword(s):  
Antiproliferative Activity ◽  
Binding Mode ◽  
Intracellular Distribution ◽  
Strong Impact ◽  
Rna Sequences

A strong impact of fluorophores’ charge and length on the binding mode, intracellular distribution and antiproliferative activity; intriguing theragnostic potential.

scholarly journals Intracellular distribution of the U1A protein depends on active transport and nuclear binding to U1 snRNA.

1992 ◽  
Vol 118 (1) ◽  
pp. 11-21 ◽  
Author(s):  
C Kambach ◽  
I W Mattaj
Keyword(s):  
Nuclear Localization ◽  
Binding Sites ◽  
Specific Protein ◽  
Intracellular Distribution ◽  
Active Process ◽  
Rna Sequences ◽  
Sequence Element ◽  
U1 Snrna ◽  
U1 Snrnp ◽  
U1a Protein

Nuclear transport of the U1 snRNP-specific protein U1A has been examined. U1A moves to the nucleus by an active process which is independent of interaction with U1 snRNA. Nuclear localization requires an unusually large sequence element situated between amino acids 94 and 204 of the protein. U1A transport is not unidirectional. The protein shuttles between nucleus and cytoplasm. At equilibrium, the concentration of the protein in the nucleus and cytoplasm is not, however, determined solely by transport rates, but can be perturbed by introducing RNA sequences that can specifically bind U1A in either the nuclear or cytoplasmic compartment. Thus, U1A represents a novel class of protein which shuttles between cytoplasm and nucleus and whose intracellular distribution can be altered by the number of free binding sites for the protein present in the cytoplasm or the nucleus.

scholarly journals Discovery of Novel Non-Steroidal Cytochrome P450 17A1 Inhibitors as Potential Prostate Cancer Agents

2020 ◽  
Vol 21 (14) ◽  
pp. 4868
Author(s):  
Tomasz M. Wróbel ◽  
Oksana Rogova ◽  
Kasper L. Andersen ◽  
Rahul Yadav ◽  
Simone Brixius-Anderko ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cytochrome P450 ◽  
Cell Lines ◽  
Antiproliferative Activity ◽  
Enzyme Inhibitors ◽  
Prostate Cancer Cell ◽  
Binding Mode ◽  
Prostate Cancer Cell Lines ◽  
Design Synthesis ◽  
Key Enzyme

The current study presents the design, synthesis, and evaluation of novel cytochrome P450 17A1 (CYP17A1) ligands. CYP17A1 is a key enzyme in the steroidogenic pathway that produces androgens among other steroids, and it is implicated in prostate cancer. The obtained compounds are potent enzyme inhibitors (sub µM) with antiproliferative activity in prostate cancer cell lines. The binding mode of these compounds is also discussed.

scholarly journals Guanidiniocarbonylpyrrole-Aryl Derivatives: Structure Tuning for Spectrophotometric Recognition of Specific DNA and RNA Sequences and for Antiproliferative Activity

2010 ◽  
Vol 16 (10) ◽  
pp. 3036-3056 ◽  
Author(s):  
Laura Hernandez-Folgado ◽  
Domagoj Baretić ◽  
Ivo Piantanida ◽  
Marko Marjanović ◽  
Marijeta Kralj ◽  
...  
Keyword(s):  
Antiproliferative Activity ◽  
Rna Sequences ◽  
Dna And Rna

scholarly journals New Samarium(III), Gadolinium(III), and Dysprosium(III) Complexes of Coumarin-3-Carboxylic Acid as Antiproliferative Agents

2007 ◽  
Vol 2007 ◽  
pp. 1-8 ◽  
Author(s):  
Irena Kostova ◽  
Georgi Momekov ◽  
Peya Stancheva
Keyword(s):  
Carboxylic Acid ◽  
Antiproliferative Activity ◽  
Myeloid Leukemia ◽  
Lanthanide Complexes ◽  
Binding Mode ◽  
Bidentate Coordination ◽  
Metal Nitrates ◽  
Samarium Complex ◽  
Physicochemical Data ◽  
Ir And Raman

New complexes of samarium(III), gadolinium(III), and dysprosium(III) with coumarin-3-carboxylic acid (HCCA) were prepared by the reaction of the ligand with respective metal nitrates in ethanol. The structures of the final complexes were determined by means of physicochemical data, elemental analysis, IR and Raman spectra. The metal-ligand binding mode in the new Ln(III) complexes of coumarin-3-carboxylic acid was elucidated. The vibrational study gave evidence for bidentate coordination of CCA− to Ln(III) ions through the carbonylic oxygen and the carboxylic oxygen atoms. The complexes were tested for antiproliferative activitiy on the chronic myeloid leukemia-derived K-562, overexpressing the BCR-ABL fusion protein. Cytotoxicity towards tumor cells was determined for a broad concentration range. The samarium salt exerted a very weak antiproliferative effect on these cells. This is in contrast to the lanthanide complexes, especially samarium complex, which exhibited potent antiproliferative activity. The present study confirms our previous observations that the lanthanide complexes of coumarins exhibit antiproliferative activity towards K-562 cell line.

High-resolution nucleic acid sequence mapping via in situ hybridization at the Electron Microscope level

1995 ◽  
Vol 53 ◽  
pp. 752-753
Author(s):  
B.A. Hamkalo ◽  
S. Narayanswami ◽  
A.P. Kausch
Keyword(s):  
Nucleic Acid ◽  
Interphase Nucleus ◽  
Genome Mapping ◽  
Precise Location ◽  
Electron Microscope Level ◽  
Rna Sequences ◽  
Fundamental Interest ◽  
Whole Cells ◽  
Dna And Rna

The availability of nonradioactive methods to label nucleic acids an the resultant rapid and greater sensitivity of detection has catapulted the technique of in situ hybridization to become the method of choice to locate of specific DNA and RNA sequences on chromosomes and in whole cells in cytological preparations in many areas of biology. It is being applied to problems of fundamental interest to basic cell and molecular biologists such as the organization of the interphase nucleus in the context of putative functional domains; it is making major contributions to genome mapping efforts; and it is being applied to the analysis of clinical specimens. Although fluorescence detection of nucleic acid hybrids is routinely used, certain questions require greater resolution. For example, very closely linked sequences may not be separable using fluorescence; the precise location of sequences with respect to chromosome structures may be below the resolution of light microscopy(LM); and the relative positions of sequences on very small chromosomes may not be feasible.

Water soluble palladium(ii) and platinum(ii) acyclic diaminocarbene complexes: solution behavior, DNA binding, and antiproliferative activity

2020 ◽  
Vol 44 (15) ◽  
pp. 5762-5773 ◽  
Author(s):  
Tatiyana V. Serebryanskaya ◽  
Mikhail A. Kinzhalov ◽  
Vladimir Bakulev ◽  
Georgii Alekseev ◽  
Anastasiya Andreeva ◽  
...  
Keyword(s):  
Dna Binding ◽  
Antiproliferative Activity ◽  
Water Soluble ◽  
Solution Behavior ◽  
Antitumor Potential ◽  
Acyclic Diaminocarbene

Water soluble Pd(ii) and Pt(ii)–ADC species synthesized via the metal-mediated coupling of isocyanides and 1,2-diaminobenzene have demonstrated antitumor potential.

In vitro and in vivo antiproliferative activity of arucanolide isolated from Calea pinnatifida

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
G Marchetti ◽  
K Silva ◽  
A Ruiz ◽  
I Sousa ◽  
S Tinti ◽  
...  
Keyword(s):  
Antiproliferative Activity
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