A cell-permeant small molecule for the super-resolution imaging of the endoplasmic reticulum in live cells

2019 ◽  
Vol 17 (15) ◽  
pp. 3732-3736 ◽  
Author(s):  
Adiki Raja Sekhar ◽  
Bhagaban Mallik ◽  
Vimlesh Kumar ◽  
Jeyaraman Sankar
Keyword(s):  
Endoplasmic Reticulum ◽  
High Resolution ◽  
Small Molecule ◽  
Super Resolution ◽  
Live Cells ◽  
High Resolution Imaging ◽  
A Cell ◽  
Resolution Imaging

A simple BODIPY-based small molecule has been identified to selectively label the endoplasmic reticulum for high-resolution imaging with negligible cytotoxicity.

scholarly journals Improved spatial resolution by induced live cell and organelle swelling in hypotonic solutions

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Astha Jaiswal ◽  
Christian H. Hoerth ◽  
Ana M. Zúñiga Pereira ◽  
Holger Lorenz
Keyword(s):  
Endoplasmic Reticulum ◽  
Cost Effectiveness ◽  
High Resolution ◽  
Light Microscopy ◽  
Matrix Protein ◽  
Super Resolution ◽  
Ease Of Use ◽  
Live Cells ◽  
Cell Organelles ◽  
Morphology Changes

Abstract Induced morphology changes of cells and organelles are by far the easiest way to determine precise protein sub-locations and organelle quantities in light microscopy. By using hypotonic solutions to swell mammalian cell organelles we demonstrate that precise membrane, lumen or matrix protein locations within the endoplasmic reticulum, Golgi and mitochondria can reliably be established. We also show the benefit of this approach for organelle quantifications, especially for clumped or intertwined organelles like peroxisomes and mitochondria. Since cell and organelle swelling is reversible, it can be applied to live cells for successive high-resolution analyses. Our approach outperforms many existing imaging modalities with respect to resolution, ease-of-use and cost-effectiveness without excluding any co-utilization with existing optical (super)resolution techniques.

High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein

2008 ◽  
Vol 1 (43) ◽  
pp. pl3-pl3 ◽  
Author(s):  
G. Maulucci ◽  
V. Labate ◽  
M. Mele ◽  
E. Panieri ◽  
G. Arcovito ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Redox Signaling ◽  
Live Cells ◽  
High Resolution Imaging ◽  
Resolution Imaging

scholarly journals High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Shihomi Masuda ◽  
Yuhki Yanase ◽  
Eiji Usukura ◽  
Sou Ryuzaki ◽  
Pangpang Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Gold Nanoparticle ◽  
High Resolution Imaging ◽  
Two Dimensional ◽  
A Cell ◽  
Resolution Imaging

scholarly journals Characterization of Cell-Induced Astigmatism in High-Resolution Imaging.

2021 ◽  
Author(s):  
Rick Rodrigues de Mercado ◽  
Hedde van Hoorn ◽  
Martin de Valois ◽  
Claude Backendorf ◽  
Julia Eckert ◽  
...  
Keyword(s):  
High Resolution ◽  
Figure Of Merit ◽  
Biological Process ◽  
Surrounding Medium ◽  
Super Resolution ◽  
High Resolution Imaging ◽  
Cell Organelles ◽  
Induced Astigmatism ◽  
Resolution Imaging

High-resolution and super-resolution techniques become more frequently used in thick, inhomogeneous samples. In particular for imaging life cells and tissue in which one wishes to observe a biological process at minimal interference and in the natural environment, sample inhomogeneities are unavoidable. Yet sample-inhomogeneities are paralleled by refractive index variations, for example between the cell organelles and the surrounding medium, that will result in the refraction of light, and therefore lead to sample-induced astigmatism. Astigmatism in turn will result in positional inaccuracies of observations that are at the heart of all super-resolution techniques. Here we introduce a simple model and define a figure-of-merit that allows one to quickly assess the importance of astigmatism for a given experimental setting. We found that astigmatism caused by the cell's nucleus can easily lead to aberrations up to hundreds of nanometers, well beyond the accuracy of all super-resolution techniques. The astigmatism generated by small objects, like bacteria or vesicles, appear to be small enough to be of any significance in typical super-resolution experimentation.

Pharmacological chaperoning of nicotinic receptors begins in the endoplasmic reticulum: High-resolution imaging

2009 ◽  
Vol 78 (7) ◽  
pp. 900-901
Author(s):  
Rigo Pantoja ◽  
Rahul Srinivasan ◽  
Sindhuja Kadambi ◽  
Elisha D.W. Mackey ◽  
Shelly Tzlil ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
High Resolution ◽  
Nicotinic Receptors ◽  
High Resolution Imaging ◽  
Resolution Imaging
Sign in / Sign up

Export Citation Format

Share Document