An artificial cell system for biocompatible gene delivery in cancer therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (18) ◽  
pp. 10189-10195 ◽  
Author(s):  
Xin Zhao ◽  
Dongyang Tang ◽  
Ying Wu ◽  
Shaoqing Chen ◽  
Cheng Wang
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Cell System ◽  
Artificial Cell

The artifical cell system for the gene therapy of cancer might be a promising approach for the reversal of neoplastic progress of cancer cells.

scholarly journals Doxorubicin Improves Cancer Cell Targeting by Filamentous Phage Gene Delivery Vectors

2020 ◽  
Vol 21 (21) ◽  
pp. 7867
Author(s):  
Effrosyni Tsafa ◽  
Kaoutar Bentayebi ◽  
Supachai Topanurak ◽  
Teerapong Yata ◽  
Justyna Przystal ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Nuclear Accumulation ◽  
Filamentous Phage ◽  
Αvβ3 Integrin ◽  
Cancer Resistance ◽  
Adeno Associated Virus ◽  
Phage Gene ◽  
Phage Capsid

Merging targeted systemic gene delivery and systemic chemotherapy against cancer, chemovirotherapy, has the potential to improve chemotherapy and gene therapy treatments and overcome cancer resistance. We introduced a bacteriophage (phage) vector, named human adeno-associated virus (AAV)/phage or AAVP, for the systemic targeting of therapeutic genes to cancer. The vector was designed as a hybrid between a recombinant adeno-associated virus genome (rAAV) and a filamentous phage capsid. To achieve tumor targeting, we displayed on the phage capsid the double-cyclic CDCRGDCFC (RGD4C) ligand that binds the alpha-V/beta-3 (αvβ3) integrin receptor. Here, we investigated a combination of doxorubicin chemotherapeutic drug and targeted gene delivery by the RGD4C/AAVP vector. Firstly, we showed that doxorubicin boosts transgene expression from the RGD4C/AAVP in two-dimensional (2D) cell cultures and three-dimensional (3D) tumor spheres established from human and murine cancer cells, while preserving selective gene delivery by RGD4C/AAVP. Next, we confirmed that doxorubicin does not increase vector attachment to cancer cells nor vector cell entry. In contrast, doxorubicin may alter the intracellular trafficking of the vector by facilitating nuclear accumulation of the RGD4C/AAVP genome through destabilization of the nuclear membrane. Finally, a combination of doxorubicin and RGD4C/AAVP-targeted suicide gene therapy exerts a synergistic effect to destroy human and murine tumor cells in 2D and 3D tumor sphere settings.

High-Effective Gene Delivery Based on Cyclodextrins Multivalent Assembly in Target Cancer Cells

2022 ◽  
Author(s):  
Yao-Hua Liu ◽  
Yu Liu
Keyword(s):  
Gene Therapy ◽  
Controlled Release ◽  
Gene Delivery ◽  
Nucleic Acids ◽  
Cancer Cells ◽  
Chemical Biology ◽  
Supramolecular Assembly ◽  
Target Cancer

Nucleic acids condensation and controlled release remain significant challenges of gene therapy in chemical biology and nanotechnology fields. In this work, we have reported a polysaccharide supramolecular assembly constructed by...

Co-Delivery of Paclitaxel and siRNA with pH-Responsive Polymeric Micelles for Synergistic Cancer Therapy

2021 ◽  
Vol 17 (2) ◽  
pp. 322-329
Author(s):  
Liuqi Shi ◽  
Huayang Feng ◽  
Zhanrong Li ◽  
Jun Shi ◽  
Lin Jin ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Polymeric Micelles ◽  
Subsurface Layer ◽  
Polymeric Micelle ◽  
Or Gene ◽  
Polymeric Vectors

Due to the complex physiological characteristics of tumors, chemotherapy or gene therapy alone cannot completely kill tumor cells. Therefore, combining chemotherapy with gene therapy for combination therapy is the key to solving this problem. However, there are still significant challenges in how to simultaneously deliver and rapidly release the drugs and siRNA into cancer cells. In this work, a triblock copolymer was synthesized to co-deliver siRNA and paclitaxel to tumor cells. This system has an acid-sensitive subsurface layer, which can not only load siRNA to prevent premature drug release but also has good controlled release performance. In vitro experiments showed that polymeric vectors can efficiently deliver siRNA and paclitaxel simultaneously into tumor cells for rapid release within the tumor cells. This study reveals that this novel polymeric micelle is a suitable vector for the codelivery of chemotherapeutic drugs and siRNA to cancer cells, representing an important advance in nanotechnology, nanomedicine, drug delivery, and cancer therapy.

scholarly journals Protein Liposomes-Mediated Targeted Acetylcholinesterase Gene Delivery for Effective Liver Cancer Therapy

2021 ◽  
Author(s):  
Kai Wang ◽  
Fusheng Shang ◽  
Dagui Chen ◽  
Tieliu Cao ◽  
Xiaowei Wang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Liver Cancer ◽  
Targeted Delivery ◽  
Tumor Targeting ◽  
Transfection Efficiency ◽  
Subcutaneous Administration ◽  
Viral Gene ◽  
Functional Protein

Abstract Effective methods to deliver therapeutic genes to solid tumors and improve their bioavailability are the main challenges of current medical research on gene therapy. The development of efficient non-viral gene vector with tumor-targeting has very important application value in the field of cancer therapy. Proteolipid integrated with tumor-targeting potential of functional protein and excellent gene delivery performance has shown potential for targeted gene therapy.Results: Herein, we prepared transferrin-modified liposomes (Tf-PL) for the targeted delivery of acetylcholinesterase (AChE) therapeutic gene to liver cancer. We found that the derived Tf-PL/AChE liposomes exhibited much higher transfection efficiency than the commercial product Lipo 2000 and shown premium targeting efficacy to liver cancer SMMC-7721 cells in vitro. In vivo, the Tf-PL/AChE could effectively target liver cancer, and significantly inhibit the growth of liver cancer xenografts grafted in nude mice by subcutaneous administration. Conclusion: This study proposed a transferrin-modified proteolipid-mediated gene delivery strategy for targeted liver cancer treatment, which has a promising potential for precise personalized cancer therapy.

Graphene oxide–hydroxyapatite nanocomposites effectively deliver HSV-TK suicide gene to inhibit human breast cancer growth

2018 ◽  
Vol 33 (2) ◽  
pp. 216-226 ◽  
Author(s):  
Tuck-Yun Cheang ◽  
Yi-Yan Lei ◽  
Zhan-Qiang Zhang ◽  
Hong-Yan Zhou ◽  
Run-Yi Ye ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Graphene Oxide ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Transfection Efficiency ◽  
Suicide Gene ◽  
Suicide Gene Therapy ◽  
Human Breast ◽  
Kinase Gene ◽  
Ganciclovir Treatment

Gene therapy with herpes simplex virus thymidine kinase gene (HSV-TK), which is also known as “suicide” gene therapy, is effective in various tumor models. The lack of a safe and efficient gene delivery system has become a major obstacle to “suicide” gene therapy. In this study, the cytotoxicity and transfection efficiency of graphene oxide–hydroxyapatite (GO–Hap) were analyzed by MTS and flow cytometry, respectively. A series of assays were performed to evaluate the effects of GO–HAp/p-HRE/ERE-Sur-TK combined with ganciclovir treatment on growth of human breast normal and cancer cells. The results showed that GO–HAp nanocomposites effectively transfected cells with minimum toxicity. GO–HAp/p-HRE/ERE-Sur-TK combined with ganciclovir treatment inhibited the proliferation and induced cell apoptosis in cancer cells, while the cytotoxic effects are tolerable in normal breast cells. We conclude that the GO–HAp nanocomposites have significant potential as a gene delivery vector for cancer therapy.

scholarly journals Protein liposomes-mediated targeted acetylcholinesterase gene delivery for effective liver cancer therapy

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Kai Wang ◽  
Fusheng Shang ◽  
Dagui Chen ◽  
Tieliu Cao ◽  
Xiaowei Wang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Liver Cancer ◽  
Targeted Delivery ◽  
Tumor Targeting ◽  
Transfection Efficiency ◽  
Subcutaneous Administration ◽  
Viral Gene ◽  
Functional Protein

Abstract Background Effective methods to deliver therapeutic genes to solid tumors and improve their bioavailability are the main challenges of current medical research on gene therapy. The development of efficient non-viral gene vector with tumor-targeting has very important application value in the field of cancer therapy. Proteolipid integrated with tumor-targeting potential of functional protein and excellent gene delivery performance has shown potential for targeted gene therapy. Results Herein, we prepared transferrin-modified liposomes (Tf-PL) for the targeted delivery of acetylcholinesterase (AChE) therapeutic gene to liver cancer. We found that the derived Tf-PL/AChE liposomes exhibited much higher transfection efficiency than the commercial product Lipo 2000 and shown premium targeting efficacy to liver cancer SMMC-7721 cells in vitro. In vivo, the Tf-PL/AChE could effectively target liver cancer, and significantly inhibit the growth of liver cancer xenografts grafted in nude mice by subcutaneous administration. Conclusions This study proposed a transferrin-modified proteolipid-mediated gene delivery strategy for targeted liver cancer treatment, which has a promising potential for precise personalized cancer therapy.

scholarly journals Targeted Protein Liposomes-Mediated AChE Gene Therapy for Effective Liver Cancer Treatment

2020 ◽  
Author(s):  
Kai Wang ◽  
Fusheng Shang ◽  
Dagui Chen ◽  
Tieliu Cao ◽  
Xiaowei Wang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Liver Cancer ◽  
Cancer Treatment ◽  
Targeted Delivery ◽  
Tumor Targeting ◽  
Transfection Efficiency ◽  
Subcutaneous Administration ◽  
Viral Gene

Abstract Background: Effective methods to deliver therapeutic genes to solid tumors and improve their bioavailability are the main challenges of current medical research on gene therapy. The development of efficient non-viral gene vector with tumor-targeting has very important application value in the field of cancer therapy. Proteolipid integrated with tumor-targeting potential of functional protein and excellent gene delivery performance has shown potential for targeted gene therapy.Results: Herein, we prepared transferrin-modified liposomes (Tf-PL) for the targeted delivery of acetylcholinesterase (AChE) therapeutic gene to liver cancer. We found that the derived Tf-PL/AChE liposomes exhibited much higher transfection efficiency than the commercial product Lipo 2000 and shown premium targeting efficacy to liver cancer SMMC-7721 cells in vitro. In vivo, the Tf-PL/AChE could effectively target liver cancer, and significantly inhibit the growth of liver cancer xenografts grafted in nude mice by subcutaneous administration. Conclusion: This study proposed a transferrin-modified proteolipid-mediated gene delivery strategy for targeted liver cancer treatment, which has a promising potential for precise personalized cancer therapy.

scholarly journals Review on Liposome as Novel Approach for Cancer Therapy

2020 ◽  
Vol 8 (3) ◽  
pp. 122-129
Author(s):  
Lodhi Devendra Singh ◽  
Kuldeep Ganju ◽  
Ramkumar Rajpoot
Keyword(s):  
Gene Therapy ◽  
Cancer Therapy ◽  
Cancer Cells ◽  
Drug Targeting ◽  
Research Work ◽  
Active Targeting ◽  
Malignant Cells ◽  
Novel Approach ◽  
Abnormal Cells ◽  
Living With Cancer

The main objective is to study the importance of liposome in drug targeting in cancer cells andgo through various research work going through on liposome. The importance of liposomes to study the flexibility to couple with the specific ligands toachieve active targeting. Cancer can be treated by surgery, radiation, chemotherapy, gene therapy, immunotherapy, hormone therapy. Cancer is the uncontrolled growth of abnormal cell anywhere in a body. The abnormal cells aretermed cancer cells, malignant cells, or tumor cells. There were 14.1 million new cancer cases, 8.2 million cancer deaths and 32.6 million people living with cancer (within 5 years of diagnosis) in 2018 worldwide. 57% (8 million) of new cancer cases, 65% (5.3 million) of the cancer deaths and 48% (15.6 million) of the 5-year prevalent cancer cases occurred in the less developed regions.    

Dextranated poly(urethane amine)s designed for systemic gene delivery in ovarian cancer therapy

2017 ◽  
Vol 5 (30) ◽  
pp. 6119-6127 ◽  
Author(s):  
Jie Zhao ◽  
Fei Han ◽  
Peng Zhao ◽  
Xuejun Wen ◽  
Chao Lin
Keyword(s):  
Ovarian Cancer ◽  
Gene Therapy ◽  
Cancer Therapy ◽  
Gene Delivery ◽  
Cancer Gene Therapy ◽  
Cancer Gene ◽  
Systemic Gene Delivery

Dextranated poly(urethane amine)s can be designed for robust ovarian cancer gene therapy.

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