Graphene oxide/alginate composites as novel bioinks for three-dimensional mesenchymal stem cell printing and bone regeneration applications

Nanoscale ◽  
2019 ◽  
Vol 11 (48) ◽  
pp. 23275-23285 ◽  
Author(s):  
Goeun Choe ◽  
Seulgi Oh ◽  
Ji Min Seok ◽  
Su A Park ◽  
Jae Young Lee
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
Cell Printing ◽  
Versatile Technique ◽  
The Creation

Three-dimensional (3D) cell printing is a versatile technique enabling the creation of 3D constructs containing hydrogel and cells in the desired shape or pattern.

scholarly journals Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis

2019 ◽  
Vol 20 (16) ◽  
pp. 3970 ◽  
Author(s):  
Souta Motoike ◽  
Mikihito Kajiya ◽  
Nao Komatsu ◽  
Susumu Horikoshi ◽  
Tomoya Ogawa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
Host Cells ◽  
Dependent Manner ◽  
Serum Free ◽  
Serum Free Conditions ◽  
Free Serum

Three-dimensional clumps of mesenchymal stem cell (MSC)/extracellular matrix (ECM) complexes (C-MSCs) consist of cells and self-produced ECM. We demonstrated previously that C-MSCs can be transplanted into bone defect regions with no artificial scaffold to induce bone regeneration. To apply C-MSCs in a clinical setting as a reliable bone regenerative therapy, the present study aimed to generate C-MSCs in xeno-free/serum-free conditions that can exert successful bone regenerative properties and to monitor interactions between grafted cells and host cells during bone healing processes. Human bone marrow-derived MSCs were cultured in xeno-free/serum-free medium. To obtain C-MSCs, confluent cells that had formed on the cellular sheet were scratched using a micropipette tip and then torn off. The sheet was rolled to make a round clump of cells. Then, C-MSCs were transplanted into an immunodeficient mouse calvarial defect model. Transplantation of C-MSCs induced bone regeneration in a time-dependent manner. Immunofluorescence staining showed that both donor human cells and host mice cells contributed to bone reconstruction. Decellularized C-MSCs implantation failed to induce bone regeneration, even though the host mice cells can infiltrate into the defect area. These findings suggested that C-MSCs generated in xeno-free/serum-free conditions can induce bone regeneration via direct and indirect osteogenesis.

scholarly journals Locally controlling mesenchymal stem cell morphogenesis by 3D PDGF-BB gradients towards the establishment of an in vitro perivascular niche

2015 ◽  
Vol 7 (1) ◽  
pp. 101-111 ◽  
Author(s):  
P. S. Lienemann ◽  
Y. R. Devaud ◽  
R. Reuten ◽  
B. R. Simona ◽  
M. Karlsson ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Cell Morphogenesis ◽  
Perivascular Niche ◽  
Tissue Model ◽  
The Creation

We report on the creation of a three dimensional biomimetic tissue model that recapitulates the stable PDGF-BB gradient controlling mesenchymal stem cell morphogenetic behavior in the perivascular niche.

Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior

2021 ◽  
Vol 4 (2) ◽  
pp. 1319-1329
Author(s):  
Minami Yoshida ◽  
Paul R. Turner ◽  
M. Azam Ali ◽  
Jaydee D. Cabral
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Cell Behavior ◽  
Chitosan Scaffolds

A cancellous bone matrix system with specific mineralisation degrees for mesenchymal stem cell differentiation and bone regeneration

2019 ◽  
Vol 7 (6) ◽  
pp. 2452-2467 ◽  
Author(s):  
Shijie Liu ◽  
Yiyun Wang ◽  
Jian Wang ◽  
Pengcheng Qiu ◽  
Shengyu Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
Bone Matrix ◽  
Stem Cell Differentiation ◽  
Matrix System ◽  
Mesenchymal Stem Cell Differentiation ◽  
Regenerative Therapies

Bone regenerative therapies have been explored using various biomaterial systems.

scholarly journals IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4+ T cells

2016 ◽  
Vol 23 (12) ◽  
pp. 1941-1951 ◽  
Author(s):  
Jiajia Xu ◽  
Yiyun Wang ◽  
Jing Li ◽  
Xudong Zhang ◽  
Yiyun Geng ◽  
...  
Keyword(s):  
T Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Regeneration ◽  
Cd4 T Cells

Fabrication of three-dimensional tissues with multilayer mesenchymal stem cell sheets: overcoming the diffusion limit

2008 ◽  
Vol 9 (2) ◽  
pp. 110 ◽  
Author(s):  
W Matsuda ◽  
Y Haraguchi ◽  
T Shimizu ◽  
S Miyoshi ◽  
A Umezawa ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Diffusion Limit ◽  
Cell Sheets

scholarly journals Effects of Co-Culture of Graphene Oxide Scaffolds with Different Concentrations and Umbilical Mesenchymal Stem Cells on the Proliferation and Differentiation of Stem Cells

2021 ◽  
Author(s):  
Aifeng Liu ◽  
Jixin Chen ◽  
Shuwei Gong ◽  
Qiang Wei ◽  
Ye Yuan
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Umbilical Cord ◽  
High Porosity ◽  
Proliferation And Differentiation ◽  
Scaffold Materials ◽  
The Impact

Abstract The main role of the scaffold materials is to enable cells to survive in the scaffold binding as while as to further promote their proliferation and differentiation ability. For mesenchymal stem cell, the scaffold could provide an environment for them to maintain their phenotype, and synthesize all necessary molecules and proteins. Generally, scaffold materials for stem cell need to possess basic characteristics such as high porosity, large surface area, surface rigidity and biodegradability. Thus, the two-dimensional graphene oxide (GO) with oxygen-containing functional groups may be suitable scaffold materials for mesenchymal stem cell culture.MethodsIn this study, the effect of GO on the value-added differentiation activity of mesenchymal stem cell was systematically investigated. ResultsIt was found that low concentration of GO and sufficient concentration of umbilical cord mesenchymal stem cells are suitable for the second Co-culture. Furthermore, the addition of hyaluronic acid will make this culture more evenly distributed. ConclusionsThe adsorption of GO on umbilical cord mesenchymal stem cells can also make the two closely linked, which avoids the impact of animal joint activities on cells.

Effects of three-dimensional spheroid culture on equine mesenchymal stem cell plasticity

2018 ◽  
Vol 42 (3) ◽  
pp. 171-181 ◽  
Author(s):  
Mi Jeong Park ◽  
Jienny Lee ◽  
Jeong Su Byeon ◽  
Da-Un Jeong ◽  
Na-Yeon Gu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Three Dimensional ◽  
Cell Plasticity ◽  
Spheroid Culture ◽  
Stem Cell Plasticity
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