Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy

Nanoscale ◽  
2019 ◽  
Vol 11 (45) ◽  
pp. 21953-21963 ◽  
Author(s):  
Xiaowei Xu ◽  
Maolei Sun ◽  
Dandan Wang ◽  
Wenhuan Bu ◽  
Zilin Wang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Osteogenic Differentiation ◽  
Silica Nanoparticles ◽  
Bone Morphogenetic Protein ◽  
Porous Silica ◽  
Bone Morphogenetic Protein 2 ◽  
Calcium Deposition ◽  
Morphogenetic Protein

Bone morphogenetic protein-2 plasmid was encapsulated by polyethylenimine-modified porous silica nanoparticles, which promoted osteogenic differentiation and increased calcium deposition with the involvement of autophagy.

scholarly journals Bone morphogenetic protein 9 induces osteogenic differentiation of germ cell 1 spermatogonial cells

2019 ◽  
Author(s):  
Jiye Zhang ◽  
Bangfa Xu ◽  
Xinping Chen ◽  
Liqiang Zhao ◽  
Pei Zhang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Germ Cell ◽  
Osteogenic Differentiation ◽  
Signaling Pathways ◽  
Bone Morphogenetic Protein ◽  
Calcium Deposition ◽  
Ectopic Ossification ◽  
Osteogenic Markers ◽  
Morphogenetic Protein

AbstractGerm cell 1 spermatogonial (GC-1spg) cells are multipotent progenitor cells. We previously confirmed that bone morphogenetic protein (BMP) 9 is among the most osteogenic BMPs. However, whether GC-1spg cells are driven toward osteogenic differentiation under proper stimuli is uncertain. Additionally, the molecular mechanism of BMP9 remains unclear. In the present study, we aimed to determine whether BMP9 can induce osteogenic differentiation of GC-1spg cells. Recombinant adenoviruses were generated by the AdEasy system to regulate the BMP9 expression in GC-1spg cells. We identified osteogenic markers by real-time PCR and staining techniques in vitro. Ectopic ossification assays and histological analysis were also performed to verify the in vivo activity of BMP9. Finally, potential signaling pathways of BMP9 were assessed by transcriptome sequencing and KEGG enrichment analysis. Using recombinant adenoviruses, we demonstrate that BMP9 upregulates osteogenic markers including Runx2, osteocalcin, osteopontin, and Sox9. BMP9 also activates alkaline phosphatase activity and calcium deposition in GC-1spg cells. In vivo results show that BMP9 overexpression in GC-1spg cells promotes ectopic bone formation and chondrogenesis. In addition, RNA-sequencing and KEGG pathway analysis demonstrate that several signaling pathways are involved in BMP9-mediated osteogenesis. GC-1spg cells not only maintain spermatogenesis but also retain the ability to form bone tissue. Therefore, BMP9 activity in GC-1spg cells may help identify signaling pathways implicated in bone formation and could be of use in regenerative medicine.

Transient Production of Bone Morphogenetic Protein 2 by Allogeneic Transplanted Transduced Cells Induces Bone Formation

2000 ◽  
Vol 11 (1) ◽  
pp. 205-211 ◽  
Author(s):  
Thomas Engstrand ◽  
Aaron Daluiski ◽  
Matthew E. Bahamonde ◽  
Hakan Melhus ◽  
Karen M. Lyons
Keyword(s):  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein
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