In situ detection of protein corona on single particle by rotational diffusivity

Nanoscale ◽  
2019 ◽  
Vol 11 (39) ◽  
pp. 18367-18374 ◽  
Author(s):  
Xijian Lin ◽  
Qi Pan ◽  
Yan He
Keyword(s):  
Diffusion Coefficient ◽  
Single Particle ◽  
Rotational Diffusion ◽  
Homogeneous Solution ◽  
Protein Corona ◽  
Thickness Change ◽  
In Situ Detection ◽  
Particle Thickness ◽  
Rotational Diffusion Coefficient

The volume-dependent rotational diffusion coefficient of gold nanorod was used to monitor the formation of protein corona in homogeneous solution in real time. The detection of particle thickness change could reach subnanometer sensitivity.

scholarly journals Particle-by-Particle in Situ Characterization of the Protein Corona via Real-Time 3D Single Particle Tracking Microscopy

2021 ◽  
Author(s):  
Xiaochen Tan ◽  
Kevin Welsher
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Fluids ◽  
Protein Corona ◽  
Single Particle Tracking ◽  
Ex Situ ◽  
High Background ◽  
Particle Nature

<p>Nanoparticles (NPs) adsorb proteins when exposed to biological fluids, forming a dynamic protein corona that affects their fate in biological environments. A comprehensive understanding of the protein corona is lacking due to the inability of current techniques to precisely measure the full corona <i>in situ</i> at the single particle level. Herein, we introduce a 3D real-time single-particle tracking spectroscopy to "lock-on" to single freely-diffusing polystyrene NPs and probe their individual protein coronas. The diffusive motions of the tracked NPs enable quantification of the "hard corona" using mean-squared displacement analysis. Critically, this method's particle-by-particle nature enabled a lock-in-type frequency filtering approach to extract the full protein corona, despite the typically confounding effect of high background signal from unbound proteins. From these results, the dynamic <i>in situ </i>full protein corona is observed to contain double the number of proteins than are observed in the <i>ex situ</i> measured "hard" protein corona.</p><br>

Study on Nanoparticle Sizing Using Fluorescent Polarization Method with DNA Fluorescent Probe

2015 ◽  
Vol 9 (5) ◽  
pp. 534-540 ◽  
Author(s):  
Terutake Hayashi ◽  
◽  
Yuki Ishizaki ◽  
Masaki Michihata ◽  
Yasuhiro Takaya ◽  
...  
Keyword(s):  
Diffusion Coefficient ◽  
Fluorescent Probe ◽  
Diffusion Coefficients ◽  
Hard Spheres ◽  
Rotational Diffusion ◽  
Polarization Method ◽  
Novel Method ◽  
Rotational Diffusion Coefficients ◽  
20 Nm ◽  
Rotational Diffusion Coefficient

Fluorescent polarization methods are used to detect complementary base pairing of DNA in biological fields. These methods work by measuring the rotational diffusion coefficient of Brownian motion of the fluorescent particles in solution. The rotational diffusion coefficient corresponds to the inverse third power of diameter according to the Debye-Stokes-Einstein equation for nanoparticles as hard spheres. We develop a novel method to measure the rotational diffusion coefficient using a fluorescent probe with a DNA spacer connected to a gold nanoparticle. We studied the physical characteristics of this probe to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles with diameters ranging between 5–20 nm were measured using this developed system. In this manuscript we describe a novel fluorescent polarization method for nanoparticle sizing using a fluorescent DNA probe.

scholarly journals Particle-by-Particle in Situ Characterization of the Protein Corona via Real-Time 3D Single Particle Tracking Microscopy

2021 ◽  
Author(s):  
Xiaochen Tan ◽  
Kevin Welsher
Keyword(s):  
Real Time ◽  
Particle Tracking ◽  
Single Particle ◽  
Biological Fluids ◽  
Protein Corona ◽  
Single Particle Tracking ◽  
Ex Situ ◽  
High Background ◽  
Particle Nature

<p>Nanoparticles (NPs) adsorb proteins when exposed to biological fluids, forming a dynamic protein corona that affects their fate in biological environments. A comprehensive understanding of the protein corona is lacking due to the inability of current techniques to precisely measure the full corona <i>in situ</i> at the single particle level. Herein, we introduce a 3D real-time single-particle tracking spectroscopy to "lock-on" to single freely-diffusing polystyrene NPs and probe their individual protein coronas. The diffusive motions of the tracked NPs enable quantification of the "hard corona" using mean-squared displacement analysis. Critically, this method's particle-by-particle nature enabled a lock-in-type frequency filtering approach to extract the full protein corona, despite the typically confounding effect of high background signal from unbound proteins. From these results, the dynamic <i>in situ </i>full protein corona is observed to contain double the number of proteins than are observed in the <i>ex situ</i> measured "hard" protein corona.</p><br>

scholarly journals In situ detection of the protein corona in complex environments

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Monica Carril ◽  
Daniel Padro ◽  
Pablo del Pino ◽  
Carolina Carrillo-Carrion ◽  
Marta Gallego ◽  
...  
Keyword(s):  
Protein Corona ◽  
Complex Environments ◽  
In Situ Detection
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