Biomineralization improves the thermostability of foot-and-mouth disease virus-like particles and the protective immune response induced

Nanoscale ◽  
2019 ◽  
Vol 11 (47) ◽  
pp. 22748-22761 ◽  
Author(s):  
Ping Du ◽  
Ronghuan Liu ◽  
Shiqi Sun ◽  
Hu Dong ◽  
Ruibo Zhao ◽  
...  
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Immune Activation ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Protective Immune Response ◽  
Virus Like Particles ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Schematic description of immune activation of DCs of the thermostable biomineralized VLPs.

scholarly journals Antigenic sites of foot-and-mouth disease virus (FMDV): an analysis of the specificities of anti-FMDV antibodies after vaccination of naturally susceptible host species

2002 ◽  
Vol 83 (4) ◽  
pp. 775-782 ◽  
Author(s):  
N. Aggarwal ◽  
P. V. Barnett
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Protective Immune Response ◽  
Susceptible Host ◽  
Ruminant Species ◽  
Antigenic Sites ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Of the known neutralizing antigenic sites of foot-and-mouth disease virus (FMDV), site 1 or A, formed in part by the G–H loop of VP1, has historically been considered immunodominant because of evidence implicating its importance in the induction of a protective immune response. However, no systematic study has been done to determine the relative importance of the various specificities of antibodies against the known neutralizing antigenic sites of FMDV in the polyclonal immune response of a natural host after vaccination. In this report, we have adopted a monoclonal antibody-based competition ELISA and used antibodies specific to sites 1, 2 and 3 to provide some insight into this issue. Following vaccination of the three main target species, cattle, pigs and sheep, with an O1 serotype strain, results indicate that none of these three antigenic sites can be considered immunodominant in a polyclonal serum. Interestingly, pigs did not respond to epitopes on the carboxy terminus end of VP1 as efficiently as the ruminant species. In addition to the known sites, other as yet undefined sites might also be important in the induction of a protective immune response. Possible implications for the design of new vaccine strategies for foot-and-mouth disease are discussed.

scholarly journals Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs

Vaccines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 470
Author(s):  
Giselle Rangel ◽  
Juan Bárcena ◽  
Noelia Moreno ◽  
Carlos P. Mata ◽  
José R. Castón ◽  
...  
Keyword(s):  
Immune Response ◽  
B Cell ◽  
Disease Virus ◽  
Cell Epitope ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Virus Like Particles ◽  
Chimeric Vlps ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Currently there is a clear trend towards the establishment of virus-like particles (VLPs) as a powerful tool for vaccine development. VLPs are tunable nanoparticles that can be engineered to be used as platforms for multimeric display of foreign antigens. We have previously reported that VLPs derived from rabbit hemorrhagic disease virus (RHDV) constitute an excellent vaccine vector, capable of inducing specific protective immune responses against inserted heterologous T-cytotoxic and B-cell epitopes. Here, we evaluate the ability of chimeric RHDV VLPs to elicit immune response and protection against Foot-and-Mouth disease virus (FMDV), one of the most devastating livestock diseases. For this purpose, we generated a set of chimeric VLPs containing two FMDV-derived epitopes: a neutralizing B-cell epitope (VP1 (140–158)) and a T-cell epitope [3A (21–35)]. The epitopes were inserted joined or individually at two different locations within the RHDV capsid protein. The immunogenicity and protection potential of the chimeric VLPs were analyzed in the mouse and pig models. Herein we show that the RHDV engineered VLPs displaying FMDV-derived epitopes elicit a robust neutralizing immune response in mice and pigs, affording partial clinical protection against an FMDV challenge in pigs.

Golden-star nanoparticles as adjuvant effectively promotes immune response to foot-and-mouth disease virus-like particles vaccine

Vaccine ◽  
2018 ◽  
Vol 36 (45) ◽  
pp. 6752-6760 ◽  
Author(s):  
Zhidong Teng ◽  
Shiqi Sun ◽  
Hao Chen ◽  
Jie Huang ◽  
Ping Du ◽  
...  
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Virus Like Particles ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Immunoreactivity and trypsin sensitivity of recombinant virus-like particles of foot-and-mouth disease virus

2015 ◽  
Vol 59 (01) ◽  
pp. 84-91 ◽  
Author(s):  
S. H. BASAGOUDANAVAR ◽  
M. HOSAMANI ◽  
R. P. TAMIL ◽  
B. P. SREENIVASA ◽  
B. K. CHANDRASEKHAR ◽  
...  
Keyword(s):  
Disease Virus ◽  
Recombinant Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Virus Like Particles ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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