A nanozyme-based cascade colorimetric aptasensor for amplified detection of ochratoxin A

Nanoscale ◽  
2019 ◽  
Vol 11 (19) ◽  
pp. 9547-9555 ◽  
Author(s):  
Fengyu Tian ◽  
Jing Zhou ◽  
Bining Jiao ◽  
Yue He
Keyword(s):  
Alkaline Phosphatase ◽  
Ochratoxin A ◽  
Catalytic Reaction ◽  
Structure Switching ◽  
Colorimetric Aptasensor

A sensitive colorimetric aptasensor has been developed based on structure-switching of an OTA aptamer coupled with an alkaline phosphatase-MnO2 cascade catalytic reaction.

scholarly journals Sensitive colorimetric detection of ochratoxin A by a dual-functional Au/Fe3O4 nanohybrid-based aptasensor

RSC Advances ◽  
2019 ◽  
Vol 9 (66) ◽  
pp. 38590-38596
Author(s):  
Rong Huang ◽  
Lu Lu Xiong ◽  
Hui Hui Chai ◽  
Jing Jing Fu ◽  
Zhisong Lu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Colorimetric Detection ◽  
Dual Functional ◽  
Colorimetric Aptasensor

A novel colorimetric aptasensor based on a Au/Fe3O4 nanohybrid was developed to detect ochratoxin A (OTA).

Highly sensitive colorimetric aptasensor for ochratoxin A detection based on enzyme-encapsulated liposome

2018 ◽  
Vol 1002 ◽  
pp. 90-96 ◽  
Author(s):  
Cuiying Lin ◽  
Huixia Zheng ◽  
Mi Sun ◽  
Yajuan Guo ◽  
Fang Luo ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Highly Sensitive ◽  
Colorimetric Aptasensor

A catalytic reaction-based colorimetric assay of alkaline phosphatase activity based on oxidase-like MnO2 microspheres

2019 ◽  
Vol 11 (42) ◽  
pp. 5472-5477 ◽  
Author(s):  
Linjie Wang ◽  
Kun Ye ◽  
Jianming Pan ◽  
Hongwei Song ◽  
Xin Li ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Catalytic Reaction ◽  
Colorimetric Assay

A catalytic reaction-based colorimetric assay of alkaline phosphatase activity is developed based on oxidase-like MnO2 microspheres.

Portable detection of ochratoxin A in red wine based on a structure-switching aptamer using a personal glucometer

RSC Advances ◽  
2016 ◽  
Vol 6 (35) ◽  
pp. 29563-29569 ◽  
Author(s):  
Chunmei Gu ◽  
Feng Long ◽  
Xiaohong Zhou ◽  
Hanchang Shi
Keyword(s):  
Ochratoxin A ◽  
Red Wine ◽  
Portable Detection ◽  
Structure Switching ◽  
First Time ◽  
Personal Glucometer

A portable aptasensor for OTA detection with the use of a PGM was for the first time developed.

scholarly journals An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A

2019 ◽  
Vol 35 (11) ◽  
pp. 1221-1226 ◽  
Author(s):  
Donny Nugraha MAZAAFRIANTO ◽  
Akihiko ISHIDA ◽  
Masatoshi MAEKI ◽  
Hirofumi TANI ◽  
Manabu TOKESHI
Keyword(s):  
Electrochemical Sensor ◽  
Ochratoxin A ◽  
Simple Detection ◽  
Structure Switching

Multicolor colorimetric detection of ochratoxin A via structure-switching aptamer and enzyme-induced metallization of gold nanorods

2020 ◽  
Vol 320 ◽  
pp. 126607 ◽  
Author(s):  
Fengyu Tian ◽  
Jing Zhou ◽  
Ruijie Fu ◽  
Yongliang Cui ◽  
Qiyang Zhao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Gold Nanorods ◽  
Colorimetric Detection ◽  
Structure Switching

Label-free colorimetric aptasensor for IgE using DNA pseudoknot probe

The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3347-3351 ◽  
Author(s):  
Chia-Chen Chang ◽  
Chen-Yu Chen ◽  
Xihong Zhao ◽  
Tzu-Heng Wu ◽  
Shih-Chung Wei ◽  
...  
Keyword(s):  
Detection Limit ◽  
High Selectivity ◽  
Colorimetric Assay ◽  
Simple Design ◽  
Label Free ◽  
Low Detection Limit ◽  
Structure Switching ◽  
Colorimetric Aptasensor

A simple design of a label-free colorimetric assay for IgE was demonstrated based on a structure-switching aptamer with a low detection limit of 0.2 nM and high selectivity.

scholarly journals Nanobody-Alkaline Phosphatase Fusion Protein-Based Enzyme-Linked Immunosorbent Assay for One-Step Detection of Ochratoxin A in Rice

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 4044 ◽  
Author(s):  
Zhichang Sun ◽  
Xuerou Wang ◽  
Qi Chen ◽  
Yonghuan Yun ◽  
Zongwen Tang ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Fusion Protein ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Limit Of Detection ◽  
Binding Capacity ◽  
Cross Reactivity ◽  
Solvent Tolerance ◽  
Enzyme Linked Immunosorbent Assay ◽  
One Step

Ochratoxin A (OTA) has become one a focus of public concern because of its multiple toxic effects and widespread contamination. To monitor OTA in rice, a sensitive, selective, and one-step enzyme-linked immunosorbent assay (ELISA) using a nanobody-alkaline phosphatase fusion protein (Nb28-AP) was developed. The Nb28-AP was produced by auto-induction expression and retained an intact antigen-binding capacity and enzymatic activity. It exhibited high thermal stability and organic solvent tolerance. Under the optimal conditions, the developed assay for OTA could be finished in 20 min with a half maximal inhibitory concentration of 0.57 ng mL−1 and a limit of detection of 0.059 ng mL−1, which was 1.1 times and 2.7 times lower than that of the unfused Nb28-based ELISA. The Nb28-AP exhibited a low cross-reactivity (CR) with ochratoxin B (0.92%) and ochratoxin C (6.2%), and an ignorable CR (<0.10%) with other mycotoxins. The developed Nb-AP-based one-step ELISA was validated and compared with a liquid chromatography-tandem mass spectrometry method. The results show the reliability of Nb-AP-based one-step ELISA for the detection of OTA in rice.

scholarly journals Enzyme cascade-amplified immunoassay based on the nanobody–alkaline phosphatase fusion and MnO2 nanosheets for the detection of ochratoxin A in coffee

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21760-21766
Author(s):  
Zeling Zhang ◽  
Benchao Su ◽  
Huan Xu ◽  
Zhenyun He ◽  
Yuling Zhou ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Ochratoxin A ◽  
Selective Detection ◽  
Mno2 Nanosheets ◽  
Enzyme Cascade

Enzyme cascade-amplified immunoassay using nanobody–alkaline phosphatase fusion and MnO2 nanosheets for sensitive and selective detection of ochratoxin A in coffee.

Sign in / Sign up

Export Citation Format

Share Document