Cellular uptake of covalent and non-covalent DNA nanostructures with different sizes and geometries

Nanoscale ◽  
2019 ◽  
Vol 11 (22) ◽  
pp. 10808-10818 ◽  
Author(s):  
Sofia Raniolo ◽  
Stefano Croce ◽  
Rasmus P. Thomsen ◽  
Anders H. Okholm ◽  
Valeria Unida ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Dna Nanostructures ◽  
Intracellular Fate ◽  
In Cells

DNA nanostructures of different sizes and forms are internalized in cells through the LOX-1 receptor with different intracellular fate and lifetime.

Understanding the fate of DNA nanostructures inside the cell

2020 ◽  
Vol 8 (29) ◽  
pp. 6170-6178 ◽  
Author(s):  
Christopher M. Green ◽  
Divita Mathur ◽  
Igor L. Medintz
Keyword(s):  
Dna Nanostructures ◽  
Responsive Materials ◽  
Intracellular Fate

DNA nanostructures are highly tunable and responsive materials for diagnostic and healthcare-related applications, but their intracellular fate remains largely unknown.

PCR quantification of SiO2 particle uptake in cells in the ppb and ppm range via silica encapsulated DNA barcodes

2014 ◽  
Vol 50 (73) ◽  
pp. 10707-10709 ◽  
Author(s):  
Marcus Hoop ◽  
Daniela Paunescu ◽  
Philipp R. Stoessel ◽  
Fritz Eichenseher ◽  
Wendelin J. Stark ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Cellular Uptake ◽  
Dna Barcode ◽  
Dna Barcodes ◽  
Cell Level ◽  
Sio2 Particle ◽  
Particle Uptake ◽  
In Cells

The cellular uptake of silica nanoparticles loaded with a DNA barcode can be detected at a 10 fg per cell level utilizing qPCR analytics.

Targeted delivery via avidin fusion protein: Intracellular fate of biotinylated doxorubicin derivative and cellular uptake kinetics and biodistribution of biotinylated liposomes

2012 ◽  
Vol 47 (5) ◽  
pp. 848-856 ◽  
Author(s):  
Suvi K. Soininen ◽  
Pauliina Lehtolainen-Dalkilic ◽  
Tanja Karppinen ◽  
Tiina Puustinen ◽  
Galina Dragneva ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cellular Uptake ◽  
Targeted Delivery ◽  
Uptake Kinetics ◽  
Intracellular Fate

Cellular processing and destinies of artificial DNA nanostructures

2016 ◽  
Vol 45 (15) ◽  
pp. 4199-4225 ◽  
Author(s):  
Di Sheng Lee ◽  
Hang Qian ◽  
Chor Yong Tay ◽  
David Tai Leong
Keyword(s):  
Dna Nanotechnology ◽  
Dna Nanostructures ◽  
Panoramic View ◽  
Artificial Dna ◽  
Cellular Processing ◽  
Mechanistic Understanding ◽  
The Many ◽  
In Cells

This review gives a panoramic view of the many DNA nanotechnology applications in cells, mechanistic understanding of how and where their interactions occur and their subsequent outcomes.

Quantification of cellular uptake of DNA nanostructures by qPCR

Methods ◽  
2014 ◽  
Vol 67 (2) ◽  
pp. 193-197 ◽  
Author(s):  
Anders Hauge Okholm ◽  
Jesper Sejrup Nielsen ◽  
Mathias Vinther ◽  
Rasmus Schøler Sørensen ◽  
David Schaffert ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Dna Nanostructures

Glimpse into Cellular Internalization and Intracellular Trafficking of Lipid-Based Nanoparticles in Cancer Cells

2021 ◽  
Vol 21 ◽  
Author(s):  
Elham Kamalkazemi ◽  
Fereydoon Abedi - Gaballu ◽  
Tala Farid Mohammad Hosseini ◽  
Ali Mohammadi ◽  
Behzad Mansoori ◽  
...  
Keyword(s):  
Particle Size ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Therapeutic Agents ◽  
Cellular Internalization ◽  
Intracellular Accumulation ◽  
Coated Nanoparticles ◽  
Cargo Delivery ◽  
Anti Cancer ◽  
Intracellular Fate

: Lipid-based nanoparticles as drug delivery carriers have been mainly used for delivery of anti-cancer therapeutic agents. Lipid-based nanoparticles, due to their smaller particle size and similarity to cell membranes, are readily internalized into cancer cells. Interestingly, cancer cells also overexpress receptors for specific ligands including folic acid, hyaluronic acid, and transferrin on their surface. This allows the use of these ligands for surface modification of the lipid-based nanoparticle. These modifications then allow the specific recognition of these ligand-coated nanoparticles by their receptors on cancer cells allowing the targeted gradual intracellular accumulation of the functionalized nanoplatforms. These interactions could eventually enhance the internalization of desired drugs via increasing ligand-receptor mediated cellular uptake of the nanoplatforms. The cellular internalization of the nanoplatforms also varies and depends on their physicochemical properties including particle size, zeta potential, and shape. The cellular uptake is also influenced by the types of ligand internalization pathway utilized by cells such as phagocytosis, macropinocytosis, and multiple endocytosis pathways. In this review, we will classify and discuss lipid based nanoparticles engineered to express specific ligands, and are recognized by their receptors on cancer cell, and their cellular internalization pathways. Moreover, the intracellular fate of nanoparticles decorated with specific ligands and the best internalization pathways (caveolae mediated endocytosis) for safe cargo delivery will be discussed.

scholarly journals Erratum: Cell-permeable peptides improve cellular uptake and therapeutic gene delivery of replication-deficient viruses in cells and in vivo

2003 ◽  
Vol 9 (9) ◽  
pp. 1221-1221
Author(s):  
Jean-Philippe Gratton ◽  
Jun Yu ◽  
Jason W Griffith ◽  
Roger W Babbitt ◽  
Ramona S Scotland ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cellular Uptake ◽  
Therapeutic Gene ◽  
In Cells
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