scholarly journals Using green emitting pH-responsive nanogels to report environmental changes within hydrogels: a nanoprobe for versatile sensing

Nanoscale ◽  
2019 ◽  
Vol 11 (24) ◽  
pp. 11484-11495 ◽  
Author(s):  
Mingning Zhu ◽  
Dongdong Lu ◽  
Shanglin Wu ◽  
Qing Lian ◽  
Wenkai Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tensile Strain ◽  
Environmental Changes ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Low Concentrations ◽  
Green Fluorescent

Low concentrations of new stimuli-responsive blue-green-fluorescent nanogel probes within four different gels and stem cells reversibly report five different stimuli: cations, degradation, pH, temperature and tensile strain.

scholarly journals Smart Composite Hydrogels with pH-Responsiveness and Electrical Conductivity for Flexible Sensors and Logic Gates

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1564 ◽  
Author(s):  
Tong Wang ◽  
Xuan Zhang ◽  
Zichao Wang ◽  
Xiuzhong Zhu ◽  
Jie Liu ◽  
...  
Keyword(s):  
Environmental Changes ◽  
Logic Gates ◽  
Body Motion ◽  
Smart Composite ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Flexible Sensors ◽  
Intelligent Analysis ◽  
Wide Range ◽  
Conductive Hydrogels

Stimuli-responsive conductive hydrogels have a wide range of applications due to their intelligent sensing of external environmental changes, which are important for smart switches, soft robotics, and flexible sensors. However, designing stimuli-responsive conductive hydrogels with logical operation, such as smart switches, remains a challenge. In this study, we synthesized pH-responsive conductive hydrogels, based on the copolymer network of acrylic acid and hydroxyethyl acrylate doped with graphene oxide. Using the good flexibility and conductivity of these hydrogels, we prepared a flexible sensor that can realize the intelligent analysis of human body motion signals. Moreover, the pH-responsive conductive hydrogels were integrated with temperature-responsive conductive hydrogels to develop logic gates with sensing, analysis, and driving functions, which realized the intellectualization of conductive hydrogels.

Soft stimuli-responsive grippers and machines with high load-to-weight ratios

2019 ◽  
Vol 6 (1) ◽  
pp. 160-168 ◽  
Author(s):  
Yajuan Sun ◽  
Linfeng Chen ◽  
Yan Jiang ◽  
Xuan Zhang ◽  
Xiukai Yao ◽  
...  
Keyword(s):  
General Class ◽  
High Load ◽  
Stimuli Responsive ◽  
Ph Responsive

A general class of stimuli-responsive grippers and actuators (e.g., temperature- and pH-responsive) with surprisingly high gripping strengths is introduced.

scholarly journals Hyaluronan Regulates Cell Behavior: A Potential Niche Matrix for Stem Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mairim Alexandra Solis ◽  
Ying-Hui Chen ◽  
Tzyy Yue Wong ◽  
Vanessa Zaiatz Bittencourt ◽  
Yen-Cheng Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Drug Resistance ◽  
Tissue Regeneration ◽  
Proliferative Activity ◽  
Cell Types ◽  
Cell Behavior ◽  
Cellular Receptors ◽  
Low Concentrations ◽  
Cellular Behaviors ◽  
High Concentrations

Hyaluronan is a linear glycosaminoglycan that has received special attention in the last few decades due to its extraordinary physiological functions. This highly viscous polysaccharide is not only a lubricator, but also a significant regulator of cellular behaviors during embryogenesis, morphogenesis, migration, proliferation, and drug resistance in many cell types, including stem cells. Most hyaluronan functions require binding to its cellular receptors CD44, LYVE-1, HARE, layilin, and RHAMM. After binding, proteins are recruited and messages are sent to alter cellular activities. When low concentrations of hyaluronan are applied to stem cells, the proliferative activity is enhanced. However, at high concentrations, stem cells acquire a dormant state and induce a multidrug resistance phenotype. Due to the influence of hyaluronan on cells and tissue morphogenesis, with regards to cardiogenesis, chondrogenesis, osteogenesis, and neurogenesis, it is now been utilized as a biomaterial for tissue regeneration. This paper summarizes the most important and recent findings regarding the regulation of hyaluronan in cells.

scholarly journals Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction

2010 ◽  
Vol 10 ◽  
pp. 422-433 ◽  
Author(s):  
Cameron McDonald ◽  
Alan Mackay-Sim ◽  
Denis Crane ◽  
Wayne Murrell
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Fluorescent Protein ◽  
Genetically Engineered ◽  
Olfactory Mucosa ◽  
Cell Transplant ◽  
Adult Rat ◽  
Cardiac Ventricle ◽  
Green Fluorescent

This study examines the hypothesis that multipotent olfactory mucosal stem cells could provide a basis for the development of autologous cell transplant therapy for the treatment of heart attack. In humans, these cells are easily obtained by simple biopsy. Neural stem cells from the olfactory mucosa are multipotent, with the capacity to differentiate into developmental fates other than neurons and glia, with evidence of cardiomyocyte differentiationin vitroand after transplantation into the chick embryo. Olfactory stem cells were grown from rat olfactory mucosa. These cells are propagated as neurosphere cultures, similar to other neural stem cells. Olfactory neurospheres were grownin vitro, dissociated into single cell suspensions, and transplanted into the infarcted hearts of congeneic rats. Transplanted cells were genetically engineered to express green fluorescent protein (GFP) in order to allow them to be identified after transplantation. Functional assessment was attempted using echocardiography in three groups of rats: control, unoperated; infarct only; infarcted and transplanted. Transplantation of neurosphere-derived cells from adult rat olfactory mucosa appeared to restore heart rate with other trends towards improvement in other measures of ventricular function indicated. Importantly, donor-derived cells engrafted in the transplanted cardiac ventricle and expressed cardiac contractile proteins.

Heterogeneity in histone 2B-green fluorescent protein-retaining putative small intestinal stem cells at cell position 4 and their absence in the colon

2012 ◽  
Vol 303 (11) ◽  
pp. G1188-G1201 ◽  
Author(s):  
Kevin R. Hughes ◽  
Ricardo M. C. Gândara ◽  
Tanvi Javkar ◽  
Fred Sablitzky ◽  
Hanno Hock ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Paneth Cells ◽  
Epithelial Stem Cells ◽  
Epithelial Regeneration ◽  
Cell Position ◽  
Small Intestinal ◽  
Green Fluorescent ◽  
Dose Radiation

Stem cells have been identified in two locations in small intestinal crypts; those intercalated between Paneth cells and another population (which retains DNA label) are located above the Paneth cell zone, at cell position 4. Because of disadvantages associated with the use of DNA label, doxycycline-induced transient transgenic expression of histone 2B (H2B)-green fluorescent protein (GFP) was investigated. H2B-GFP-retaining putative stem cells were consistently seen, with a peak at cell position 4, over chase periods of up to 112 days. After a 28-day chase, a subpopulation of the H2B-GFP-retaining cells was cycling, but the slow cycling status of the majority was illustrated by lack of expression of pHistone H3 and Ki67. Although some H2B-GFP-retaining cells were sensitive to low-dose radiation, the majority was resistant to low- and high-dose radiation-induced cell death, and a proportion of the surviving cells proliferated during subsequent epithelial regeneration. Long-term retention of H2B-GFP in a subpopulation of small intestinal Paneth cells was also seen, implying that they are long lived. In contrast to the small intestine, H2B-GFP-retaining epithelial cells were not seen in the colon from 28-day chase onward. This implies important differences in stem cell function between these two regions of the gastrointestinal tract, which may have implications for region-specific susceptibility to diseases (such as cancer and ulcerative colitis), in which epithelial stem cells and their progeny are involved.

scholarly journals Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1134
Author(s):  
Julia I. Khorolskaya ◽  
Daria A. Perepletchikova ◽  
Daniel V. Kachkin ◽  
Kirill E. Zhurenkov ◽  
Elga I. Alexander-Sinkler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Fluorescent Protein ◽  
Rabbit Model ◽  
Stem Cell Markers ◽  
Epithelial Stem Cells ◽  
Limbal Stem Cell ◽  
Green Fluorescent

The development of cell-based approaches to the treatment of various cornea pathologies, including limbal stem cell deficiency (LSCD), is an area of current interest in regenerative biomedicine. In this context, the shortage of donor material is urgent, and limbal mesenchymal stem cells (L-MSCs) may become a promising cell source for the development of these novel approaches, being established mainly within the rabbit model. In this study, we obtained and characterized rabbit L-MSCs and modified them with lentiviral transduction to express the green fluorescent protein EGFP (L-MSCs-EGFP). L-MSCs and L-MSCs-EGFP express not only stem cell markers specific for mesenchymal stem cells but also ABCG2, ABCB5, ALDH3A1, PAX6, and p63a specific for limbal epithelial stem cells (LESCs), as well as various cytokeratins (3/12, 15, 19). L-MSCs-EGFP have been proven to differentiate into adipogenic, osteogenic, and chondrogenic directions, as well as to transdifferentiate into epithelial cells. The possibility of using L-MSCs-EGFP to study the biocompatibility of various scaffolds developed to treat corneal pathologies was demonstrated. L-MSCs-EGFP may become a useful tool for studying regenerative processes occurring during the treatment of various corneal pathologies, including LSCD, with the use of cell-based technologies.

Stem Cell Defect in Ubiquitin-Green Fluorescent Protein Mice Facilitates Engraftment of Lymphoid-Primed Hematopoietic Stem Cells

Stem Cells ◽  
2018 ◽  
Vol 36 (8) ◽  
pp. 1237-1248
Author(s):  
Kateřina Faltusová ◽  
Katarína Szikszai ◽  
Martin Molík ◽  
Jana Linhartová ◽  
Petr Páral ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Green Fluorescent Protein ◽  
Hematopoietic Stem Cells ◽  
Fluorescent Protein ◽  
Hematopoietic Stem ◽  
Green Fluorescent ◽  
Cell Defect

Microfluidic actuator based on stimuli-responsive hydrogel grafted into Cucurbita moschata xylems

Soft Matter ◽  
2021 ◽  
Author(s):  
Marcelo Ricardo Romero ◽  
Gisella Trejo ◽  
José Vedelago ◽  
Cesar Gomez
Keyword(s):  
Cucurbita Moschata ◽  
Stimuli Responsive ◽  
Ph Responsive ◽  
Chemically Modified ◽  
Responsive Polymer ◽  
Plant Stem

A chemical actuator was developed taking advantage of the internal microstructure of a plant stem. Stem xylems of Cucurbita moschata were chemically modified with a pH-responsive polymer to obtain an...

Sign in / Sign up

Export Citation Format

Share Document