Construction of molecularly imprinted nanoplatforms with persistent luminescence for the in vitro specific adsorption and in vivo targeted regulation of food-borne biotoxins

2019 ◽  
Vol 43 (38) ◽  
pp. 15097-15104 ◽  
Author(s):  
Jing-Min Liu ◽  
Zhi-Hao Wang ◽  
Chun-Yang Li ◽  
Shi-Wen Lv ◽  
Ning Zhao ◽  
...  
Keyword(s):  
Specific Adsorption ◽  
Persistent Luminescence ◽  
Molecularly Imprinted ◽  
Long Afterglow ◽  
Food Borne

Molecularly imprinted nanoplatforms with super-long afterglow persistent luminescence showed in vitro specific adsorption and in vivo targeted regulation ability of food-borne biotoxins by autofluorescence-free nanoimaging.

scholarly journals Probiotic and Functional Properties of Limosilactobacillus reuteri INIA P572

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1860
Author(s):  
Patricia Diez-Echave ◽  
Izaskun Martín-Cabrejas ◽  
José Garrido-Mesa ◽  
Susana Langa ◽  
Teresa Vezza ◽  
...  
Keyword(s):  
In Vitro Assays ◽  
Immunomodulatory Activity ◽  
Probiotic Properties ◽  
Protective Effects ◽  
Mice Model ◽  
In Vivo Models ◽  
Food Borne ◽  
Gastrointestinal Conditions

Limosilactobacillus reuteri INIA P572 is a strain able to produce the antimicrobial compound reuterin in dairy products, exhibiting a protective effect against some food-borne pathogens. In this study, we investigated some probiotic properties of this strain such as resistance to gastrointestinal passage or to colonic conditions, reuterin production in a colonic environment, and immunomodulatory activity, using different in vitro and in vivo models. The results showed a high resistance of this strain to gastrointestinal conditions, as well as capacity to grow and produce reuterin in a human colonic model. Although the in vitro assays using the RAW 264.7 macrophage cell line did not demonstrate direct immunomodulatory properties, the in vivo assays using a Dextran Sulphate Sodium (DSS)-induced colitic mice model showed clear immunomodulatory and protective effects of this strain.

scholarly journals Super Long Green Persistent Luminescence from X-Ray Excited β-NaYF4: Tb3+

2019 ◽  
Author(s):  
Yue Hu ◽  
Yanmin Yang ◽  
Xiaoxiao Li ◽  
Xin Wang ◽  
Yunqian Li ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Light Source ◽  
Green Emission ◽  
Persistent Luminescence ◽  
Tunneling Model ◽  
X Ray ◽  
Long Afterglow ◽  
Photo Stability ◽  
F Centers

Here, we have discovered a X-ray excited long afterglow phosphor β-NaYF4: Tb3+. After the irradiation of X-ray, the green emission can persist for more than 240 h. After 36 h, the afterglow intensity arrived at 0.69 mcd•m-2, which can clearly be observed by naked eyes. Even after 84 h, the afterglow emission brightness still reached 0.087 mcd•m-2. Also, combined with the results of thermoluminescence and photoluminescence, the super long afterglow emission of β-NaYF4: Tb3+ can be ascribed to the tunneling model associated with F centers. More importantly, the super long green afterglow emission of β-NaYF4: Tb3+ has been successfully used as in vivo light source to activate g-C3N4 for photodynamic therapy（PDT）and bacteria destruction. Furthermore, super long persistent luminescence of β-NaYF4: Tb3+ could be repeatedly charged by X-ray for many circulations, which indicates that the phosphors have high photo stability under repeated cycles of alternating X-ray irradiation.

Metal oxide nanoparticle-functionalized sebacic acid-grafted PHEAM nanocarriers for enriched activity of metronidazole against food borne bacteria: in vitro and in vivo study

2018 ◽  
Vol 42 (22) ◽  
pp. 18437-18447 ◽  
Author(s):  
Murugesan Gowri ◽  
Kannan Suganya ◽  
Nachimuthu Latha ◽  
Marudhamuthu Murugan ◽  
Mukhtar Ahmed ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Metal Oxide ◽  
Sebacic Acid ◽  
In Vivo Study ◽  
Metal Oxide Nanoparticle ◽  
Food Borne ◽  
Oxide Nanoparticle

Food borne infection is a serious complication caused by Listeria monocytogenes (L. monocytogenes), a dangerous bacteria.

scholarly journals In vitro and in vivo trematode models for chemotherapeutic studies

Parasitology ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 589-603 ◽  
Author(s):  
J. KEISER
Keyword(s):  
Liver Fluke ◽  
Fasciola Hepatica ◽  
State Of The Art ◽  
Parasitic Diseases ◽  
Current State ◽  
Food Borne ◽  
Essential Components ◽  
Discovery Pipeline

SUMMARYSchistosomiasis and food-borne trematodiases are chronic parasitic diseases affecting millions of people mostly in the developing world. Additional drugs should be developed as only few drugs are available for treatment and drug resistance might emerge. In vitro and in vivo whole parasite screens represent essential components of the trematodicidal drug discovery cascade. This review describes the current state-of-the-art of in vitro and in vivo screening systems of the blood fluke Schistosoma mansoni, the liver fluke Fasciola hepatica and the intestinal fluke Echinostoma caproni. Examples of in vitro and in vivo evaluation of compounds for activity are presented. To boost the discovery pipeline for these diseases there is a need to develop validated, robust high-throughput in vitro systems with simple readouts.

scholarly journals Development of Molecularly Imprinted Olanzapine Nano-particles: In Vitro Characterization and In Vivo Evaluation

2016 ◽  
Vol 17 (6) ◽  
pp. 1457-1467 ◽  
Author(s):  
Nersi Jafary Omid ◽  
Hoda Morovati ◽  
Mohsen Amini ◽  
Ahmad-Reza Dehpour ◽  
Alireza Partoazar ◽  
...  
Keyword(s):  
Nano Particles ◽  
In Vivo Evaluation ◽  
Molecularly Imprinted ◽  
In Vitro Characterization

scholarly journals Reg4 defenses the intestine against Salmonella infection via binding the flagellin

2021 ◽  
Author(s):  
Yongtao Xiao ◽  
weipeng wang ◽  
Ying lu ◽  
xinbei tian ◽  
shanshan chen ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Bactericidal Activity ◽  
Intestinal Inflammation ◽  
Bactericidal Effect ◽  
Intestinal Epithelia ◽  
Food Borne ◽  
Negative Impacts ◽  
First Time

Salmonella Typhimurium is gram-negative flagellated bacteria that can cause food-borne gastroenteritis and diarrhea in humans and animals. The regenerating islet-derived family member 4 (Reg4) is overexpressed in the gastrointestinal tract during intestinal inflammation. However, the role of Reg4 in the intestinal inflammation induced by Salmonella Typhimurium is largely unknown. In this study, we reported for the first time that Reg4 has bactericidal activity against intestinal infection caused by Salmonella Typhimurium. In vivo, Reg4 could reduce the colonization of Salmonella Typhimurium and attenuate intestinal inflammation in the Salmonella Typhimurium-infected model. Additionally, the mice with the epithelial cell specific deletion of Reg4 (Reg4ΔIEC) exhibited more severe intestinal inflammation and more colonization of Salmonella Typhimurium. However, the administration of Reg4 could reverse these negative impacts. In vitro, Reg4 protein was showed to inhibit the growth of Salmonella Typhimurium. We further investigate the function motif of Reg4 and find that the "HDPQK" motif in Reg4 is essential to its bactericidal activity. Reg4 exerted the bactericidal effect by binding to the flagellin of Salmonella Typhimurium and suppressing its motility, adhesion, and invasion to the intestinal epithelia. In conclusion, our findings identify Reg4 as a novel antimicrobial peptide against infection by Salmonella Typhimurium and explore its possible mechanism, which may be of great significance for developing novel agents against flagellated micro pathogens.

scholarly journals Persistent luminescence materials for deep photodynamic therapy

Nanophotonics ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2999-3029 ◽  
Author(s):  
Aurélie Bessière ◽  
Jean-Olivier Durand ◽  
Camille Noûs
Keyword(s):  
Photodynamic Therapy ◽  
Uv Light ◽  
Small Animal Imaging ◽  
Small Animal ◽  
Charge Trapping ◽  
Full Potential ◽  
Persistent Luminescence ◽  
Living Tissues

Abstract Persistent luminescence (PerL) materials continue emitting light long after their excitation has stopped. Prepared in the form of nanoparticles they revealed their full potential as bio-nanoprobes for in vivo small animal imaging in the last 15 years. PerL materials enable to overcome the limitation of weak light penetration in living tissues. As such, they constitute remarkable light mediators to implement photodynamic therapy (PDT) in deep-seated tissues. This article reviews the recent achievements in PerL-mediated PDT in vitro as well as in small animal cancer models in vivo. PerL-mediated PDT is realized through the smart choice of a tandem of a PerL material and a photosensitizer (PS). The physical association of the PerL material and the PS as well as their targeting ability is debated. Implants or mesoporous nanoparticles emerge as particularly valuable cargos that further permit multimodality in imaging or therapy. The diversity of charge-trapping mechanisms in a few PerL materials enables a large versatility in the excitation protocols. Although the PerL agent can be pre-excited by UV light before its introduction into the animal, it also induces effective PDT after simple infrared or visible LED illumination across tissues as well as after a mild X-ray irradiation.

Activity of tribendimidine and praziquantel combination therapy against the liver fluke Opisthorchis viverriniin vitro and in vivo

2012 ◽  
Vol 87 (2) ◽  
pp. 252-256 ◽  
Author(s):  
J. Keiser ◽  
R. Adelfio ◽  
M. Vargas ◽  
P. Odermatt ◽  
S. Tesana
Keyword(s):  
Worm Burden ◽  
Liver Fluke ◽  
Combination Index ◽  
Public Health Problem ◽  
Important Public Health Problem ◽  
Important Public Health ◽  
Single Drug ◽  
Food Borne

AbstractOpisthorchiasis, caused by the liver fluke Opisthorchis viverrini, a food-borne trematode, is an important public health problem; however, only a single drug, praziquantel is available. We investigated tribendimidine–praziquantel combinations against O. viverriniin vitro and in vivo. The IC50 values of 0.16 μg/ml and 0.05 μg/ml were determined for praziquantel and tribendimidine, respectively, against adult O. viverriniin vitro. When O. viverrini was exposed to both drugs simultaneously (using a drug ratio based on the IC50 (1:3.2)) a synergistic effect was calculated (combination index (CI) at the IC50= 0.7). A similar result was observed when drug addition in vitro was spaced by the respective half-lives of the drugs (a CI of 0.78 at the IC50 for tribendimidine followed by praziquantel and a CI of 0.47 at the IC50 for praziquantel followed by tribendimidine). In vivo median-effect dose (ED50) values of 191 mg/kg and 147 mg/kg were calculated for praziquantel and tribendimidine, respectively. Low to moderate worm burden reductions (38–62%) were observed in O. viverrini infected hamsters when both drugs were administered simultaneously or on subsequent days, pointing to antagonistic effects in vivo. Further studies are necessary to understand the striking differences between the in vitro and in vivo observations using combinations of praziquantel and tribendimidine on O. viverrini.

scholarly journals Fabrication of an activatable hybrid persistent luminescence nanoprobe for background-free bioimaging-guided investigation of food-borne aflatoxin in vivo

RSC Advances ◽  
2018 ◽  
Vol 8 (50) ◽  
pp. 28414-28420 ◽  
Author(s):  
Jing-Min Liu ◽  
Xin-Yue Yuan ◽  
Hui-Lin Liu ◽  
Dai Cheng ◽  
Shuo Wang
Keyword(s):  
Copper Sulfide ◽  
Persistent Luminescence ◽  
Food Borne

Construction of persistent luminescence nanophosphor-copper sulfide hybrid FRET nanoprobes for background-free bioimaging-guided investigation of food-borne aflatoxin in vivo.

scholarly journals Narrow-Spectrum Inhibitors of Campylobacter jejuni Flagellar Expression and Growth

2015 ◽  
Vol 59 (7) ◽  
pp. 3880-3886 ◽  
Author(s):  
Jeremiah G. Johnson ◽  
Caroline Yuhas ◽  
Thomas J. McQuade ◽  
Martha J. Larsen ◽  
Victor J. DiRita
Keyword(s):  
Small Molecules ◽  
Campylobacter Jejuni ◽  
Campylobacter Coli ◽  
Content Type ◽  
Food Borne ◽  
Food Borne Illness ◽  
Inhibitor Screen

ABSTRACTCampylobacter jejuniis a major cause of food-borne illness due to its ability to reside within the gastrointestinal tracts of chickens. Multiple studies have identified the flagella ofC. jejunias a major determinant of chicken colonization. An inhibitor screen of approximately 147,000 small molecules was performed to identify compounds that are able to inhibit flagellar expression in a reporter strain ofC. jejuni. Several compounds that modestly inhibited motility of wild-typeC. jejuniin standard assays were identified, as were a number of small molecules that robustly inhibitedC. jejunigrowth,in vitro. Examination of similar bacterial screens found that many of these small molecules inhibited only the growth ofC. jejuni. Follow-up assays demonstrated inhibition of other strains ofC. jejuniandCampylobacter colibut no inhibition of the closely relatedHelicobacter pylori. The compounds were determined to be bacteriostatic and nontoxic to eukaryotic cells. Preliminary results from a day-of-hatch chick model of colonization suggest that at least one of the compounds demonstrates promise for reducingCampylobactercolonization loadsin vivo, although further medicinal chemistry may be required to enhance bioavailability.

Sign in / Sign up

Export Citation Format

Share Document