Development and validation of a bioanalytical HPLC method for simultaneous estimation of cinnamaldehyde and cinnamic acid in rat plasma: application for pharmacokinetic studies

2020 ◽  
Vol 44 (11) ◽  
pp. 4346-4352
Author(s):  
Varsha Shetty ◽  
Bothiraja Chellampillai ◽  
Ruchika Kaul-Ghanekar
Keyword(s):  
Cinnamic Acid ◽  
Simultaneous Detection ◽  
Rat Plasma ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Pharmacokinetic Studies ◽  
Plasma Samples ◽  
Development And Validation

A rapid, sensitive and accessible HPLC-UV method was developed and validated for simultaneous detection of CNAD and CA up to 1.0 ng ml−1 in plasma samples.

Development and Validation of a Method for Simultaneous Estimation of Sitagliptin and Ertugliflozin in Rat Plasma by LC–MS method

2020 ◽  
Vol 16 ◽  
Author(s):  
P. Venkateswara Rao ◽  
A. Lakshmana Rao ◽  
S.V.U.M. Prasad
Keyword(s):  
Matrix Effect ◽  
Multiple Reaction Monitoring ◽  
Rat Plasma ◽  
Marketing Approval ◽  
Simultaneous Estimation ◽  
Pharmacokinetic Studies ◽  
Oral Antidiabetic Agents ◽  
Liquid Liquid Extraction ◽  
Positive Mode ◽  
Development And Validation

Background: The development of sound bioanalytical LC-MS (liquid chromatography-mass spectroscopy) method(s) is of paramount importance during the process of drug discovery, development and culminating in a marketing approval. The use of oral antidiabetic agents has been increased significantly from last decades and till now no bioanalytical method is available for quantitation of sitagliptin (SG) and ertugliflozin (EG) in biological matrix which can be applied to pharmacokinetic studies using LC-MS/MS. Objective: To develop a new, rapid and sensitive LC–MS/MS method for the simultaneous estimation of sitagliptin (SG) and ertugliflozin (EG) in rat plasma by liquid–liquid extraction method (LLE) using deutereated sitagliptin (SGd6) and ertugliflozin (EGd6). Method: Chromatographic separation was carried out on a reverse phase Waters, Xetrra C18 (150mm x 4.6mm, 2μm) column using mixture of acetonitrile and OPA buffer (50:50v/v) at a flow rate of 1ml/min in isocratic mode. Quantification was achieved using an electrospray ion interface operating in positive mode, under multiple reaction monitoring (MRM) conditions. Results: The method showed excellent linearity over the concentration range of 5.00- 75.00pg/mL for sitagliptin and 0.75- 11.35pg/mL ertugliflozin. The intra-batch and inter batch precision (%CV) was ≤ 4.3% and matrix effect (%CV) was 0.02% and 0.12% for sitagliptin at HQC and LQC, respectively. Matrix effect (%CV) was 0.08% and 0.33% for ertugliflozin at HQC and LQC, respectively. Conclusion: The simplicity of the method allows for application in laboratories, presents a valuable tool for pharmacokinetic studies. The particular assay has been proficiently put on pharmacokinetic study in rats subjects.

scholarly journals Development and validation of RP-HPLC method for sildenafil citrate in rat plasma-application to pharmacokinetic studies

2013 ◽  
Vol 21 (3) ◽  
pp. 317-321 ◽  
Author(s):  
A.S. Tripathi ◽  
I. Sheikh ◽  
A.P. Dewani ◽  
P.G. Shelke ◽  
R.L. Bakal ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Sildenafil Citrate ◽  
Pharmacokinetic Studies ◽  
Rp Hplc ◽  
Development And Validation

Development and validation of a reversed-phase HPLC method for determination of nitrendipine in rat plasma: application to pharmacokinetic studies

2007 ◽  
Vol 21 (4) ◽  
pp. 363-368 ◽  
Author(s):  
Vinay Kumar Venishetty ◽  
Chandrasekar Durairaj ◽  
Ramakrishna Sistla ◽  
Madhusudhan Rao Yamsani ◽  
Prakash V. Diwan
Keyword(s):  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Pharmacokinetic Studies ◽  
Reversed Phase Hplc ◽  
Development And Validation

scholarly journals Development and Validation of an HPLC/MS/MS Method for Determining the Thiazolidinone PG15 in Rat Plasma

2010 ◽  
Vol 93 (4) ◽  
pp. 1215-1221
Author(s):  
FláVia D T UchôA ◽  
Eduardo C Palma ◽  
Natalia F Souza ◽  
Maria C A Lima ◽  
Suely L Galdino ◽  
...  
Keyword(s):  
Internal Standard ◽  
Ammonium Hydroxide ◽  
Rat Plasma ◽  
Negative Ion ◽  
Pharmacokinetic Studies ◽  
Plasma Samples ◽  
Isocratic Elution ◽  
Negative Ion Mode ◽  
Development And Validation

Abstract A rapid, sensitive, and simple HPLC/MS/MS method was developed and validated for the determination of (5Z,E)-3-[2-(4-chlorophenyl)-2-oxoethyl]-5-(1H-indol-3-ylmethylene)-thiazolidine-2, 4-dione (PG15) in rat plasma using chlortalidone as an internal standard (IS). Analyses were performed using a C18 column and isocratic elution with acetonitrilewater (90 + 10, v/v) containing 10 mM ammonium hydroxide (pH 8.0) as the mobile phase pumped at 0.3 mL/min. Detection was performed by MS with negative ion mode electrospray ionization. Rat plasma samples were prepared by deproteinizing with acetonitrile. Detected fragments were 395.1 > 171.9 for PG15 and 337.3 > 189.9 for the IS. Calibration curves were linear from 10 to 1000 ng/mL, with the determination coefficient >0.99. The intraday and interday precisions were less than 12.2 and 11.3, respectively. The applicability of the HPLC/MS/MS method for pharmacokinetic studies was tested using plasma samples obtained after oral administration of PG15 to rats, and it provided the necessary sensitivity, linearity, precision, accuracy, and specificity.

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