scholarly journals Site-selective functionalization of plasmonic nanopores for enhanced fluorescence emission rate and Förster resonance energy transfer

2019 ◽  
Vol 1 (6) ◽  
pp. 2454-2461 ◽  
Author(s):  
Xavier Zambrana-Puyalto ◽  
Nicolò Maccaferri ◽  
Paolo Ponzellini ◽  
Giorgia Giovannini ◽  
Francesco De Angelis ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Emission Rate ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Enhanced Fluorescence ◽  
Selective Functionalization ◽  
A Site ◽  
Site Selective ◽  
Nanoscale Level

A site-selective functionalization method in a plasmonic nanopore demonstrates how it is possible to modulate FRET at the nanoscale level.

scholarly journals Resonance energy transfer at percolation transition

2020 ◽  
Vol 35 (03) ◽  
pp. 2040022
Author(s):  
P. P. Abrantes ◽  
D. Szilard ◽  
F. S. S. Rosa ◽  
C. Farina
Keyword(s):  
Energy Transfer ◽  
Density Of States ◽  
Emission Rate ◽  
Local Density ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Local Density Of States ◽  
Percolation Transition ◽  
Spontaneous Emission Rate ◽  
Metallic Inclusions

We compute the resonance energy transfer (RET) in a system composed of two quantum emitters near a host dielectric matrix in which metallic inclusions are inserted until the medium undergoes a dielectric-metal transition at percolation. We show that there is no peak in the RET rate at percolation, in contrast to what happens with the spontaneous emission rate of an emitter near the same critical medium. This result suggests that RET does not strongly correlate with the local density of states.

Plasmon‐Enhanced Fluorescence Resonance Energy Transfer

2019 ◽  
Vol 19 (5) ◽  
pp. 818-842 ◽  
Author(s):  
Huan Zong ◽  
Xinxin Wang ◽  
Xijiao Mu ◽  
Jingang Wang ◽  
Mengtao Sun
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Enhanced Fluorescence ◽  
Plasmon Enhanced Fluorescence ◽  
Fluorescence Resonance

scholarly journals A quantitative protocol for dynamic measurements of protein interactions by Förster resonance energy transfer-sensitized fluorescence emission

2008 ◽  
Vol 6 (suppl_1) ◽  
Author(s):  
A.D Elder ◽  
A Domin ◽  
G.S Kaminski Schierle ◽  
C Lindon ◽  
J Pines ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Protein Interactions ◽  
Imaging Techniques ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Confocal Imaging ◽  
Förster Resonance Energy Transfer ◽  
Förster Resonance

Fluorescence detection of acceptor molecules sensitized by Förster resonance energy transfer (FRET) is a powerful method to study protein interactions in living cells. The method requires correction for donor spectral bleed-through and acceptor cross-excitation as well as the correct normalization of signals to account for varying fluorophore concentrations and imaging parameters. In this paper, we review different methods for FRET signal normalization and then present a rigorous model for sensitized emission measurements, which is both intuitive to understand and practical to apply. The method is validated by comparison with the acceptor photobleaching and donor lifetime-imaging techniques in live cell samples containing EYFP and ECFP tandem constructs exhibiting known amounts of FRET. By varying the stoichiometry of interaction in a controlled fashion, we show that information on the fractions of interacting donors and acceptors can be recovered. Furthermore, the method is tested by performing measurements on different microscopy platforms in both widefield and confocal imaging modes to show that signals recovered under different imaging conditions are in quantitative agreement. Finally, the method is applied in the study of dynamic interactions in the cyclin–cdk family of proteins in live cells. By normalizing the obtained signals for both acceptor and donor concentrations and using a FRET exhibiting control construct for calibration, stoichiometric changes in these interactions could be visualized in real time. The paper is written to be of practical use to researchers interested in performing sensitized emission measurements. The correct interpretation of the retrieved signals in a biological context is emphasized, and guidelines are given for the practical application of the developed algorithms.

scholarly journals Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Sensors ◽  
2020 ◽  
Vol 20 (10) ◽  
pp. 2909 ◽  
Author(s):  
Anirban Samanta ◽  
Igor L. Medintz
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Organic Dyes ◽  
Stokes Shift ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Semiconductor Quantum Dots ◽  
Renilla Luciferase ◽  
Bioluminescent Protein

Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect: that the excited donor here is produced by biochemical means and not by an external illumination. Often the choice of BRET source is the bioluminescent protein Renilla luciferase, which catalyzes the oxidation of a substrate, typically coelenterazine, producing an oxidized product in its electronic excited state that, in turn, couples with a proximal fluorophore resulting in a fluorescence emission from the acceptor. The acceptors pertinent to this discussion are semiconductor quantum dots (QDs), which offer some unrivalled photophysical properties. Amongst other advantages, the QD’s large Stokes shift is particularly advantageous as it allows easy and accurate deconstruction of acceptor signal, which is difficult to attain using organic dyes or fluorescent proteins. QD-BRET systems are gaining popularity in non-invasive bioimaging and as probes for biosensing as they don’t require external optical illumination, which dramatically improves the signal-to-noise ratio by avoiding background auto-fluorescence. Despite the additional advantages such systems offer, there are challenges lying ahead that need to be addressed before they are utilized for translational types of research.

Understanding of Förster Resonance Energy Transfer (FRET) in Ionic Materials

2021 ◽  
Vol 2 (4) ◽  
pp. 564-575
Author(s):  
Amanda Jalihal ◽  
Thuy Le ◽  
Samantha Macchi ◽  
Hannah Krehbiel ◽  
Mujeebat Bashiru ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nile Blue ◽  
Photophysical Properties ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Fluorescence Emission Spectrum ◽  
Förster Resonance

Herein, an ionic material (IM) with Förster Resonance Energy Transfer (FRET) characteristics is reported for the first time. The IM is designed by pairing a Nile Blue A cation (NBA+) with an anionic near-infrared (NIR) dye, IR820−, using a facile ion exchange reaction. These two dyes absorb at different wavelength regions. In addition, NBA+ fluorescence emission spectrum overlaps with IR820− absorption spectrum, which is one requirement for the occurrence of the FRET phenomenon. Therefore, the photophysical properties of the IM were studied in detail to investigate the FRET mechanism in IM for potential dye sensitized solar cell (DSSCs) application. Detailed examination of photophysical properties of parent compounds, a mixture of the parent compounds, and the IM revealed that the IM exhibits FRET characteristics, but not the mixture of two dyes. The presence of spectator counterion in the mixture hindered the FRET mechanism while in the IM, both dyes are in close proximity as an ion pair, thus exhibiting FRET. All FRET parameters such as spectral overlap integral, Förster distance, and FRET energy confirm the FRET characteristics of the IM. This article presents a simple synthesis of a compound with FRET properties which can be further used for a variety of applications.

FLUORESCENCE RESONANCE ENERGY TRANSFER BETWEEN DYES AND WATER-SOLUBLE QUANTUM DOTS WITH AVIDIN AS A BRIDGE

2004 ◽  
Vol 03 (03) ◽  
pp. 273-280
Author(s):  
QI-DAN CHEN ◽  
ZHANG-BI LIN ◽  
XING-GUANG SU ◽  
HAO ZHANG ◽  
XIAO-HONG HE ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Fluorescence Emission ◽  
Resonance Energy ◽  
Water Soluble ◽  
Fluorescence Emission Spectrum ◽  
Fluorescence Characteristics ◽  
Fluorescence Resonance

3-Mercaptopropyl acid-capped quantum dots (QDs) synthesized in aqueous solution were coupled to avidin-sulforhodamine, also named avidin-Texas red (ATR), via electrostatic attraction. An intensity reduction in the fluorescence emission spectrum of QDs and an enhanced fluorescence intensity of the dye were observed on account of fluorescence resonance energy transfer from the QD donors to the dye acceptors. In addition, the fluorescence characteristics of the QD-ATR conjugates were strongly-related to the quantity of ATR, pH value and ionic strength.

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