pH dependence of the non-cooperative binding of Bi3+ to human apo-metallothionein 1A: kinetics, speciation, and stoichiometry

Metallomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 435-448 ◽  
Author(s):  
Natalie C. Korkola ◽  
Patti M. Scarrow ◽  
Martin J. Stillman
Keyword(s):  
Ph Dependence ◽  
Cooperative Binding ◽  
Stopped Flow ◽  
Cysteine Modification ◽  
Esi Ms ◽  
Stopped Flow Kinetics

ESI-MS along with cysteine modification show that the binding of Bi3+ to apo-metallothionein is non-cooperative with a coordination of BiS(cys)3 up to Bi6MT. Stopped flow kinetics reveal that the rate of binding depends on the pH and the Bi3+ anion.

Metallothionein Cd4S11 cluster formation dominates in the protection of carbonic anhydrase

Metallomics ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 767-783 ◽  
Author(s):  
Amelia T. Yuan ◽  
Natalie C. Korkola ◽  
Daisy L. Wong ◽  
Martin J. Stillman
Keyword(s):  
Carbonic Anhydrase ◽  
Protein Interactions ◽  
Cluster Formation ◽  
Stopped Flow ◽  
Protein Protein Interactions ◽  
Esi Ms ◽  
Stopped Flow Kinetics

Results from ESI-MS and stopped flow kinetics show that apo-MT protects from toxic metalation of apo-CA with Cd2+ due to the protein–protein interactions in solution.

Stopped-Flow Kinetics Investigation of the Imidazole-Catalyzed Peroxyoxalate Chemiluminescence Reaction

1998 ◽  
Vol 63 (9) ◽  
pp. 3023-3031 ◽  
Author(s):  
Andrew G. Hadd ◽  
Alex L. Robinson ◽  
Kathy L. Rowlen ◽  
John W. Birks
Keyword(s):  
Stopped Flow ◽  
Peroxyoxalate Chemiluminescence ◽  
Stopped Flow Kinetics

Insights into the Mechanism ofPseudomonas dacunhaeAspartate β-Decarboxylase from Rapid-Scanning Stopped-Flow Kinetics

Biochemistry ◽  
2010 ◽  
Vol 49 (24) ◽  
pp. 5066-5073 ◽  
Author(s):  
Robert S. Phillips ◽  
Santiago Lima ◽  
Roman Khristoforov ◽  
Bakthavatsalam Sudararaju
Keyword(s):  
Stopped Flow ◽  
Stopped Flow Kinetics ◽  
Rapid Scanning
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