A mini-panel PET scanner-based microfluidic radiobioassay system allowing high-throughput imaging of real-time cellular pharmacokinetics

Lab on a Chip ◽  
2020 ◽  
Vol 20 (6) ◽  
pp. 1110-1123 ◽  
Author(s):  
Zhen Liu ◽  
Pengfei Zhang ◽  
Hao Ji ◽  
Yu Long ◽  
Boping Jing ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Cellular Pharmacokinetics ◽  
System P ◽  
High Throughput Imaging

Pharmacokinetics of parallel or comparison samples can be imaged simultaneously with a mini PET scanner-based microfluidic radiobioassay system.

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

scholarly journals Real-time imaging of cellular forces using optical interference

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Andrew T. Meek ◽  
Nils M. Kronenberg ◽  
Andrew Morton ◽  
Philipp Liehm ◽  
Jan Murawski ◽  
...  
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
High Throughput ◽  
Mechanical Activity ◽  
Dynamic Processes ◽  
Imaging Method ◽  
Neonatal Cardiomyocytes ◽  
A Cell ◽  
Cellular Forces ◽  
Time Acquisition

AbstractImportant dynamic processes in mechanobiology remain elusive due to a lack of tools to image the small cellular forces at play with sufficient speed and throughput. Here, we introduce a fast, interference-based force imaging method that uses the illumination of an elastic deformable microcavity with two rapidly alternating wavelengths to map forces. We show real-time acquisition and processing of data, obtain images of mechanical activity while scanning across a cell culture, and investigate sub-second fluctuations of the piconewton forces exerted by macrophage podosomes. We also demonstrate force imaging of beating neonatal cardiomyocytes at 100 fps which reveals mechanical aspects of spontaneous oscillatory contraction waves in between the main contraction cycles. These examples illustrate the wider potential of our technique for monitoring cellular forces with high throughput and excellent temporal resolution.

scholarly journals Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction

BioTechniques ◽  
2005 ◽  
Vol 38 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Van Luu-The ◽  
Nathalie Paquet ◽  
Ezequiel Calvo ◽  
Jean Cumps
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Second Derivative ◽  
Rt Pcr ◽  
Pcr Method

Demonstration of real-time pattern correction for high-throughput maskless lithography

2011 ◽  
Author(s):  
E. A. Hakkennes ◽  
A. D. Wiersma ◽  
M. Hoving ◽  
N. Venema ◽  
S. Woutersen ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Maskless Lithography ◽  
Time Pattern

scholarly journals Inertial Microfluidic Cell Stretcher (iMCS): Fully Automated, High-Throughput, and Near Real-Time Cell Mechanotyping

Small ◽  
2017 ◽  
Vol 13 (28) ◽  
pp. 1700705 ◽  
Author(s):  
Yanxiang Deng ◽  
Steven P. Davis ◽  
Fan Yang ◽  
Kevin S. Paulsen ◽  
Maneesh Kumar ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Cell Stretcher
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