scholarly journals A bifurcated continuous field-flow fractionation (BCFFF) chip for high-yield and high-throughput nucleic acid extraction and purification

Lab on a Chip ◽  
2019 ◽  
Vol 19 (22) ◽  
pp. 3853-3861 ◽  
Author(s):  
Chenguang Zhang ◽  
Gongchen Sun ◽  
Satyajyoti Senapati ◽  
Hsueh-Chia Chang
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
High Yield ◽  
Acid Extraction ◽  
Field Flow Fractionation ◽  
Nucleic Acid Extraction ◽  
Isolation And Purification ◽  
Extraction And Purification ◽  
Flow Fractionation ◽  
Continuous Field

We report a new Bifurcated Continuous Field-Flow Fractionation (BCFFF) microfluidic chip for isolation and purification of nucleic acids from blood plasma with high and concentration-independent yield. The platform is ideal for isolation and quantification of small miRNAs.

scholarly journals Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCR

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 615
Author(s):  
Allen Wing-Ho Chu ◽  
Cyril Chik-Yan Yip ◽  
Wan-Mui Chan ◽  
Anthony Chin-Ki Ng ◽  
Dream Lok-Sze Chan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Rna Extraction ◽  
Nasopharyngeal Swab ◽  
Acid Extraction ◽  
Rt Pcr ◽  
Nucleic Acid Extraction ◽  
Suitable Alternative ◽  
Pcr Inhibitors ◽  
Pcr Targeting

SARS-CoV-2 RT-PCR with pooled specimens has been implemented during the COVID-19 pandemic as a cost- and manpower-saving strategy for large-scale testing. However, there is a paucity of data on the efficiency of different nucleic acid extraction platforms on pooled specimens. This study compared a novel automated high-throughput liquid-based RNA extraction (LRE) platform (PHASIFYTM) with a widely used magnetic bead-based total nucleic acid extraction (MBTE) platform (NucliSENS® easyMAG®). A total of 60 pools of nasopharyngeal swab and 60 pools of posterior oropharyngeal saliva specimens, each consisting of 1 SARS-CoV-2 positive and 9 SARS-CoV-2 negative specimens, were included for the comparison. Real-time RT-PCR targeting the SARS-CoV-2 RdRp/Hel gene was performed, and GAPDH RT-PCR was used to detect RT-PCR inhibitors. No significant differences were observed in the Ct values and overall RT-PCR positive rates between LRE and MBTE platforms (92.5% (111/120] vs 90% (108/120]), but there was a slightly higher positive rate for LRE (88.3% (53/60]) than MBTE (81.7% (49/60]) among pooled saliva. The automated LRE method is comparable to a standard MBTE method for the detection of SAR-CoV-2 in pooled specimens, providing a suitable alternative automated extraction platform. Furthermore, LRE may be better suited for pooled saliva specimens due to more efficient removal of RT-PCR inhibitors.

Synergistic use of electroosmotic flow and magnetic forces for nucleic acid extraction

The Analyst ◽  
2020 ◽  
Vol 145 (6) ◽  
pp. 2412-2419 ◽  
Author(s):  
Rachel N. Deraney ◽  
Lindsay Schneider ◽  
Anubhav Tripathi
Keyword(s):  
Nucleic Acid ◽  
Electric Field ◽  
Microfluidic Chip ◽  
Applied Electric Field ◽  
Electroosmotic Flow ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Magnetic Forces ◽  
Extraction And Purification

NA extraction and purification utilitzing a microfluidic chip with applied electric field to induce electroosmotic flow opposite the magnetic NA-bound bead mix.

scholarly journals NEATTILL: A simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications

Plant Methods ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
Yellamaraju Sreelakshmi ◽  
Soni Gupta ◽  
Reddaiah Bodanapu ◽  
Vineeta Chauhan ◽  
Mickey Hanjabam ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Acid Extraction ◽  
Reverse Genetic ◽  
Nucleic Acid Extraction ◽  
Simplified Procedure

scholarly journals Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation

PLoS Biology ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. e3000107 ◽  
Author(s):  
Phil Oberacker ◽  
Peter Stepper ◽  
Donna M. Bond ◽  
Sven Höhn ◽  
Jule Focken ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Magnetic Beads ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Open Platform

Inexpensive, High Throughput Microplate Format for Plant Nucleic Acid Extraction: Suitable for Multiplex Southern Analyses of Transgenes

Crop Science ◽  
2005 ◽  
Vol 45 (5) ◽  
pp. 1985-1989 ◽  
Author(s):  
L. Flagel ◽  
J. R. Christensen ◽  
C. D. Gustus ◽  
K. P. Smith ◽  
P. M. Olhoft ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Acid Extraction ◽  
Nucleic Acid Extraction

scholarly journals High-throughput HTLV-1 proviral DNA detection system using a nucleic acid extraction robot and real-time PCR detection.

2001 ◽  
Vol 47 (3) ◽  
pp. 378-383 ◽  
Author(s):  
Chieko Matsumoto ◽  
Rieko Shiozawa ◽  
Shigeki Mitsunaga ◽  
Akiko Ichikawa ◽  
Rika Ishiwatari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Detection System ◽  
Dna Detection ◽  
Pcr Detection ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Proviral Dna

scholarly journals Automated TruTip nucleic acid extraction and purification from raw sputum

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199869 ◽  
Author(s):  
Nitu Thakore ◽  
Ryan Norville ◽  
Molly Franke ◽  
Roger Calderon ◽  
Leonid Lecca ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Extraction And Purification

scholarly journals Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique

Micromachines ◽  
2017 ◽  
Vol 8 (7) ◽  
pp. 228 ◽  
Author(s):  
Darren Branch ◽  
Erika Vreeland ◽  
Jamie McClain ◽  
Jaclyn Murton ◽  
Conrad James ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Extraction ◽  
Ultrasonic Technique ◽  
Nucleic Acid Extraction ◽  
Extraction And Purification

Advances in Fully Automated Nucleic Acid Extraction System Based on Magnetic Nanobeads

2019 ◽  
Vol 11 (12) ◽  
pp. 1633-1643
Author(s):  
Zhu Chen ◽  
Changhu Xiao ◽  
Manling Tang ◽  
Yuyue Xu ◽  
Ziyu He ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Throughput ◽  
Point Of Care ◽  
Low Cost ◽  
Future Trend ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Advantages And Disadvantages ◽  
Good Repeatability ◽  
Magnetic Nanobeads

Using magnetic nanobeads (MNBs) to extract nucleic acids is an efficient, inexpensive, easy automation, high throughput and good repeatability, instead of the traditional nucleic acid extraction (NAE) methods. Advances in fully automated MNBs-based nucleic acid extraction systems (MNAES) can push the frontiers of point-of-care testing (POCT) devices towards low-cost, automation, and enhanced accuracy molecular-level diagnostics. So, this paper introduces the pipettingbased MNAES with position of magnetic separation kit or tip, and based on magnetic bar MNAES with blending manner is shock or rotation. Further, advantages and disadvantages of various MNAES are compared. We envisage that the future trend in molecular diagnosis and monitoring will be facilitation, intelligent, miniaturization, and high throughput MNAES with sample-in-answer-out capability.

Sign in / Sign up

Export Citation Format

Share Document