scholarly journals Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples

Lab on a Chip ◽  
2019 ◽  
Vol 19 (20) ◽  
pp. 3375-3386 ◽  
Author(s):  
Elizabeth A. Phillips ◽  
Taylor J. Moehling ◽  
Karin F. K. Ejendal ◽  
Orlando S. Hoilett ◽  
Kristin M. Byers ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Cell ◽  
Cell Separation ◽  
Nucleic Acid Amplification ◽  
Viral Lysis ◽  
Blood Samples ◽  
Isothermal Nucleic Acid Amplification ◽  
Whole Blood Samples ◽  
Capillary Fluidics ◽  
Analytical Device

This handheld sample-to-answer platform combines blood cell separation, viral lysis, and isothermal nucleic acid amplification with capillary fluidics and heating controls to automatically detect HIV from blood samples within 90 minutes.

scholarly journals Rapid Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection

2018 ◽  
Vol 64 (3) ◽  
pp. 556-565 ◽  
Author(s):  
Ji Yeun Kim ◽  
Bonhan Koo ◽  
Choong Eun Jin ◽  
Min Chul Kim ◽  
Yong Pil Chong ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Scrub Typhus ◽  
Nucleic Acid Amplification ◽  
Blood Samples ◽  
Clinical Sensitivity ◽  
Isothermal Nucleic Acid Amplification ◽  
One Step ◽  
Sensor Detection

Abstract BACKGROUND Scrub typhus and severe fever with thrombocytopenia syndrome (SFTS) are the most common tick-borne illnesses in South Korea. Early differentiation of SFTS from scrub typhus in emergency departments is essential but difficult because of their overlapping epidemiology, shared risk factors, and similar clinical manifestations. METHODS We compared the diagnostic performance of one-step isothermal nucleic acid amplification with bio-optical sensor detection (iNAD) under isothermal conditions, which is rapid (20–30 min), with that of real-time PCR, in patients with a confirmed tick-borne illness. Fifteen patients with confirmed SFTS who provided a total of 15 initial blood samples and 5 follow-up blood samples, and 21 patients with confirmed scrub typhus, were evaluated. RESULTS The clinical sensitivity of iNAD (100%; 95% CI, 83–100) for SFTS was significantly higher than that of real-time PCR (75%; 95% CI, 51–91; P = 0.047), while its clinical specificity (86%; 95% CI, 65–97) was similar to that of real-time PCR (95%; 95% CI, 77–99; P = 0.61). The clinical sensitivity of iNAD for scrub typhus (100%; 95% CI, 81–100) was significantly higher than that of real-time PCR for scrub typhus (67%; 95% CI, 43–85; P = 0.009), while its clinical specificity (90%; 95% CI, 67–98) was similar to that of real-time PCR (95%; 95% CI, 73–100; P > 0.99). CONCLUSIONS iNAD is a valuable, rapid method of detecting SFTS virus and Orientia tsutsugamushi with high clinical sensitivity and specificity.

Electricity-free chemical heater for isothermal nucleic acid amplification with applications in COVID-19 home testing

The Analyst ◽  
2021 ◽  
Author(s):  
Rui Jie Li ◽  
Michael G. Mauk ◽  
Youngung Seok ◽  
Haim H. Bau
Keyword(s):  
Nucleic Acid ◽  
Phase Change ◽  
Phase Change Material ◽  
Heat Generation ◽  
Temperature Regulation ◽  
Nucleic Acid Amplification ◽  
Enzymatic Amplification ◽  
Isothermal Nucleic Acid Amplification ◽  
Home Testing ◽  
Change Material

Electricty-free incubation of isothermal enzymatic amplification with a composite comprised of exothermic reactants for heat generation and phase change material for temperature regulation.

scholarly journals Rapid electrostatic DNA enrichment for sensitive detection of Trichomonas vaginalis in clinical urinary samples

2020 ◽  
Vol 12 (8) ◽  
pp. 1085-1093
Author(s):  
Justin M. Rosenbohm ◽  
James M. Robson ◽  
Rishabh Singh ◽  
Rose Lee ◽  
Jane Y. Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Trichomonas Vaginalis ◽  
Nucleic Acid Amplification ◽  
Urine Samples ◽  
Sensitive Detection ◽  
Isothermal Nucleic Acid Amplification

Rapid electrostatic enrichment of DNA from urine samples for improved isothermal nucleic acid amplification-based detection of Trichomonas vaginalis.

scholarly journals Interfacial Effect-Based Quantification of Droplet Isothermal Nucleic Acid Amplification for Bacterial Infection

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Tiffany-Heather Ulep ◽  
Alexander S. Day ◽  
Katelyn Sosnowski ◽  
Alexa Shumaker ◽  
Jeong-Yeol Yoon
Keyword(s):  
Nucleic Acid ◽  
Bacterial Infection ◽  
Nucleic Acid Amplification ◽  
Interfacial Effect ◽  
Isothermal Nucleic Acid Amplification
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