scholarly journals Smartphone-app based point-of-care testing for myocardial infarction biomarker cTnI using an autonomous capillary microfluidic chip with self-aligned on-chip focusing (SOF) lenses

Lab on a Chip ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 1797-1807 ◽  
Author(s):  
Chao Liang ◽  
Yuanchang Liu ◽  
Aiying Niu ◽  
Chong Liu ◽  
Jingmin Li ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Fluorescence Intensity ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
The Self ◽  
Smartphone App ◽  
Point Of Care Testing ◽  
On Chip

We present a smartphone-app platform for point-of-care testing of cTnI, which features the self-aligned on-chip focusing (SOF) lenses for enhancing the fluorescence intensity.

scholarly journals Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections

2021 ◽  
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Mingzhu Yang ◽  
Changcheng Qian ◽  
A S M Muhtasim Fuad Sohan
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Promising Alternative ◽  
Reactive Protein ◽  
Linear Relationships ◽  
Multiple Biomarkers

Abstract Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.

Point-of-Care Systems for Rapid DNA Quantification in Oncology

2008 ◽  
Vol 94 (2) ◽  
pp. 216-225 ◽  
Author(s):  
Marco Bianchessi ◽  
Sarah Burgarella ◽  
Marco Cereda
Keyword(s):  
Point Of Care ◽  
Low Cost ◽  
Semiconductor Industry ◽  
Point Of Care Testing ◽  
Lab On Chip ◽  
Diagnostic Assays ◽  
Care Systems ◽  
Point Of Care Systems ◽  
The Common ◽  
On Chip

The development of new powerful applications and the improvement in fabrication techniques are promising an explosive growth in lab-on-chip use in the upcoming future. As the demand reaches significant levels, the semiconductor industry may enter in the field, bringing its capability to produce complex devices in large volumes, high quality and low cost. The lab-on-chip concept, when applied to medicine, leads to the point-of-care concept, where simple, compact and cheap instruments allow diagnostic assays to be performed quickly by untrained personnel directly at the patient's side. In this paper, some practical and economical considerations are made to support the advantages of point-of-care testing. A series of promising technologies developed by STMicroelectronics on lab-on-chips is also presented, mature enough to enter in the common medical practice. The possible use of these techniques for cancer research, diagnosis and treatment are illustrated together with the benefits offered by their implementation in point-of-care testing.

A Shake&Read distance-based microfluidic chip as a portable quantitative readout device for highly sensitive point-of-care testing

2016 ◽  
Vol 52 (91) ◽  
pp. 13377-13380 ◽  
Author(s):  
Yi Xie ◽  
Xiaofeng Wei ◽  
Qizhen Yang ◽  
Zhichao Guan ◽  
Dan Liu ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Point Of Care ◽  
Point Of Care Testing ◽  
Readout Device ◽  
Highly Sensitive ◽  
Disposable Equipment

We developed a Shake&Read distance-based microfluidic chip for simple, disposable, equipment-free, visual and quantitative POCT.

Saliva-based creatine kinase MB measurement as a potential point-of-care testing for detection of myocardial infarction

2011 ◽  
Vol 16 (3) ◽  
pp. 775-779 ◽  
Author(s):  
Iraj Mirzaii-Dizgah ◽  
Seyed Fakhreddin Hejazi ◽  
Esmail Riahi ◽  
Mohammad Mohsen Salehi
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Point Of Care ◽  
Point Of Care Testing ◽  
Creatine Kinase Mb

scholarly journals Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing

2015 ◽  
Vol 7 (2) ◽  
pp. 025007 ◽  
Author(s):  
Hojjat Madadi ◽  
Jasmina Casals-Terré ◽  
Mahdi Mohammadi
Keyword(s):  
Blood Plasma ◽  
Microfluidic Chip ◽  
Point Of Care ◽  
Point Of Care Testing
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