A numbering-up strategy of hydrodynamic microfluidic filters for continuous-flow high-throughput cell sorting

Lab on a Chip ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 1828-1837 ◽  
Author(s):  
Ryoken Ozawa ◽  
Hideki Iwadate ◽  
Hajime Toyoda ◽  
Masumi Yamada ◽  
Minoru Seki
Keyword(s):  
High Throughput ◽  
Cell Sorting ◽  
Continuous Flow ◽  
Particle Sorting

A numbering-up strategy of hydrodynamic filters was presented to dramatically increase the throughput of cell/particle sorting up to ∼15 mL min−1.

scholarly journals Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1000-1003 ◽  
Author(s):  
Seung-Yong Jung ◽  
Timothy Notton ◽  
Erika Fong ◽  
Maxim Shusteff ◽  
Leor S. Weinberger
Keyword(s):  
High Throughput ◽  
Cell Sorting ◽  
Sonic Velocity ◽  
Particle Sorting ◽  
Spatial Tuning ◽  
Fluidic Device

We report a two-channel fluidic device that allows geometry-independent tuning of acoustic nodes for high-throughput particle sorting.

scholarly journals Continuous-flow microfluidic blood cell sorting for unprocessed whole blood using surface-micromachined microfiltration membranes

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2565-2575 ◽  
Author(s):  
Xiang Li ◽  
Weiqiang Chen ◽  
Guangyu Liu ◽  
Wei Lu ◽  
Jianping Fu
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Microfluidic Chip ◽  
Cell Sorting ◽  
Whole Blood ◽  
Continuous Flow ◽  
Microfiltration Membranes

A microfluidic chip for continuous-flow isolation of WBCs from unprocessed whole blood with high throughput and purity.

scholarly journals A High-Throughput Screening System Based on Fluorescence-Activated Cell Sorting for the Directed Evolution of Chitinase A

2021 ◽  
Vol 22 (6) ◽  
pp. 3041
Author(s):  
Gheorghita Menghiu ◽  
Vasile Ostafe ◽  
Radivoje Prodanović ◽  
Rainer Fischer ◽  
Raluca Ostafe
Keyword(s):  
High Throughput ◽  
Cell Sorting ◽  
High Throughput Screening ◽  
Screening Method ◽  
Enzymatic Reaction ◽  
Hydrolytic Enzymes ◽  
Screening System ◽  
Fluorescence Activated Cell Sorting ◽  
Fungal Cell ◽  
Chitinase A

Chitinases catalyze the degradation of chitin, a polymer of N-acetylglucosamine found in crustacean shells, insect cuticles, and fungal cell walls. There is great interest in the development of improved chitinases to address the environmental burden of chitin waste from the food processing industry as well as the potential medical, agricultural, and industrial uses of partially deacetylated chitin (chitosan) and its products (chito-oligosaccharides). The depolymerization of chitin can be achieved using chemical and physical treatments, but an enzymatic process would be more environmentally friendly and more sustainable. However, chitinases are slow-acting enzymes, limiting their biotechnological exploitation, although this can be overcome by molecular evolution approaches to enhance the features required for specific applications. The two main goals of this study were the development of a high-throughput screening system for chitinase activity (which could be extrapolated to other hydrolytic enzymes), and the deployment of this new method to select improved chitinase variants. We therefore cloned and expressed the Bacillus licheniformis DSM8785 chitinase A (chiA) gene in Escherichia coli BL21 (DE3) cells and generated a mutant library by error-prone PCR. We then developed a screening method based on fluorescence-activated cell sorting (FACS) using the model substrate 4-methylumbelliferyl β-d-N,N′,N″-triacetyl chitotrioside to identify improved enzymes. We prevented cross-talk between emulsion compartments caused by the hydrophobicity of 4-methylumbelliferone, the fluorescent product of the enzymatic reaction, by incorporating cyclodextrins into the aqueous phases. We also addressed the toxicity of long-term chiA expression in E. coli by limiting the reaction time. We identified 12 mutants containing 2–8 mutations per gene resulting in up to twofold higher activity than wild-type ChiA.

scholarly journals Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

Nanoscale ◽  
2017 ◽  
Vol 9 (36) ◽  
pp. 13592-13599 ◽  
Author(s):  
Qian Zhang ◽  
Ting Yin ◽  
Rongrong Xu ◽  
Wenjun Gao ◽  
Hui Zhao ◽  
...  
Keyword(s):  
T Cells ◽  
Clinical Application ◽  
High Throughput ◽  
Cell Sorting ◽  
Large Scale ◽  
Magnetic Cell Sorting ◽  
Potential Clinical Application

A self-designed high-throughput system has been developed for large-scale immuno-magnetic cell sorting of different T cells.

A continuous-flow microfluidic syringe filter for size-based cell sorting

Lab on a Chip ◽  
2015 ◽  
Vol 15 (5) ◽  
pp. 1250-1254 ◽  
Author(s):  
Seungjeong Song ◽  
Minseok S. Kim ◽  
Jaeyeon Lee ◽  
Sungyoung Choi
Keyword(s):  
Cell Cycle ◽  
Cell Sorting ◽  
Continuous Flow ◽  
Robust Approach ◽  
Cell Cycle Synchronization ◽  
Syringe Filter

This communication presents a microfluidic method for size-based cell sorting, which provides a simple and robust approach for cell cycle synchronization by manual and stand-alone operation.

Controlled photo-flow oxidative reaction (UV-FOR) platform for ultra-fast phthalide and API synthesis

2018 ◽  
Vol 20 (20) ◽  
pp. 4584-4590 ◽  
Author(s):  
Dnyaneshwar Aand ◽  
Sanjeev Karekar ◽  
Bhushan Mahajan ◽  
Amit B. Pawar ◽  
Ajay K. Singh
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Continuous Flow ◽  
Oxidative Reaction

A time-efficient, integrated and continuous-flow platform has been developed for real-time, high-throughput studies on phthalide and API synthesis from ketone–O2 reactions.

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