scholarly journals Biological explorations with nanoscale secondary ion mass spectrometry

2019 ◽  
Vol 34 (8) ◽  
pp. 1534-1545 ◽  
Author(s):  
Frank Gyngard ◽  
Matthew L. Steinhauser
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Secondary Ion Mass Spectrometry ◽  
Cell Populations ◽  
Biological Processes ◽  
Ion Mass Spectrometry ◽  
Secondary Ion ◽  
Subcellular Level

Investigation of biological processes at the single cell or subcellular level with methods such as NanoSIMS is critical in order to better understand heterogeneous cell populations.

Single cell lipidomics of SKBR-3 breast cancer cells by using time-of-flight secondary-ion mass spectrometry

2014 ◽  
Vol 46 (S1) ◽  
pp. 181-184 ◽  
Author(s):  
Yoshimi Ide ◽  
Michihiko Waki ◽  
Itsuko Ishizaki ◽  
Yasuyuki Nagata ◽  
Fumiyoshi Yamazaki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Single Cell ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

scholarly journals Insights into Carbon Metabolism Provided by FluorescenceIn SituHybridization-Secondary Ion Mass Spectrometry Imaging of an Autotrophic, Nitrate-Reducing, Fe(II)-Oxidizing Enrichment Culture

2018 ◽  
Vol 84 (9) ◽  
Author(s):  
Claudia Tominski ◽  
Tina Lösekann-Behrens ◽  
Alexander Ruecker ◽  
Nikolas Hagemann ◽  
Sara Kleindienst ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Single Cell ◽  
Secondary Ion Mass Spectrometry ◽  
Enrichment Culture ◽  
Content Type ◽  
Cell Counts ◽  
Ion Mass Spectrometry ◽  
Card Fish ◽  
The Impact ◽  
Secondary Ion

ABSTRACTThe enrichment culture KS is one of the few existing autotrophic, nitrate-reducing, Fe(II)-oxidizing cultures that can be continuously transferred without an organic carbon source. We used a combination of catalyzed amplification reporter deposition fluorescencein situhybridization (CARD-FISH) and nanoscale secondary ion mass spectrometry (NanoSIMS) to analyze community dynamics, single-cell activities, and interactions among the two most abundant microbial community members (i.e.,Gallionellaceaesp. andBradyrhizobiumspp.) under autotrophic and heterotrophic growth conditions. CARD-FISH cell counts showed the dominance of the Fe(II) oxidizerGallionellaceaesp. under autotrophic conditions as well as ofBradyrhizobiumspp. under heterotrophic conditions. We used NanoSIMS to monitor the fate of13C-labeled bicarbonate and acetate as well as15N-labeled ammonium at the single-cell level for both taxa. Under autotrophic conditions, only theGallionellaceaesp. was actively incorporating13C-labeled bicarbonate and15N-labeled ammonium. Interestingly, bothBradyrhizobiumspp. andGallionellaceaesp. became enriched in [13C]acetate and [15N]ammonium under heterotrophic conditions. Our experiments demonstrated thatGallionellaceaesp. was capable of assimilating [13C]acetate whileBradyrhizobiumspp. were not able to fix CO2, although a metagenomics survey of culture KS recently revealed thatGallionellaceaesp. lacks genes for acetate uptake and that theBradyrhizobiumsp. carries the genetic potential to fix CO2. The study furthermore extends our understanding of the microbial reactions that interlink the nitrogen and Fe cycles in the environment.IMPORTANCEMicrobial mechanisms by which Fe(II) is oxidized with nitrate as the terminal electron acceptor are generally referred to as “nitrate-dependent Fe(II) oxidation” (NDFO). NDFO has been demonstrated in laboratory cultures (such as the one studied in this work) and in a variety of marine and freshwater sediments. Recently, the importance of NDFO for the transport of sediment-derived Fe in aquatic ecosystems has been emphasized in a series of studies discussing the impact of NDFO for sedimentary nutrient cycling and redox dynamics in marine and freshwater environments. In this article, we report results from an isotope labeling study performed with the autotrophic, nitrate-reducing, Fe(II)-oxidizing enrichment culture KS, which was first described by Straub et al. (1) about 20 years ago. Our current study builds on the recently published metagenome of culture KS (2).

Secondary‐Ion Mass Spectrometry Images Cardiolipins and Phosphatidylethanolamines at the Subcellular Level

2019 ◽  
Vol 131 (10) ◽  
pp. 3188-3193 ◽  
Author(s):  
Hua Tian ◽  
Louis J. Sparvero ◽  
Paul Blenkinsopp ◽  
Andrew A. Amoscato ◽  
Simon C. Watkins ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Ion Mass Spectrometry ◽  
Secondary Ion ◽  
Subcellular Level
Sign in / Sign up

Export Citation Format

Share Document