Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats

2020 ◽  
Vol 11 (4) ◽  
pp. 3706-3718 ◽  
Author(s):  
Min-you Qi ◽  
Xu-tao Wang ◽  
Hui-lin Xu ◽  
Zhang-liang Yang ◽  
Yin Cheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Protective Effect ◽  
Ferulic Acid ◽  
Podocyte Injury

Ferulic acid protects against diabetic nephropathy in STZ-induced rats by attenuating oxidative stress, inflammation, fibrosis and podocyte injury.

scholarly journals Protective Effect of Hydroxysafflor Yellow A on Nephropathy by Attenuating Oxidative Stress and Inhibiting Apoptosis in Induced Type 2 Diabetes in Rat

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Maosheng Lee ◽  
Hengxia Zhao ◽  
Xuemei Liu ◽  
Deliang Liu ◽  
Jianping Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Diabetic Nephropathy ◽  
Western Blot ◽  
Protective Effect ◽  
Caspase 3 ◽  
Renal Tissue ◽  
Hydroxysafflor Yellow A

Diabetic nephropathy (DN) is a serious complication of diabetes mellitus, and its prevalence has been increasing all over the world, which is also the leading cause of end-stage renal failure. Hydroxysafflor yellow A (HSYA) is the main active chemical component of Carthamus tinctorius L., and it is commonly used in patients with cardiovascular and cerebrovascular diseases in China. The aim of this study was to investigate the renal protective effects and molecular mechanisms of HSYA on high-fat diet (HFD) and streptozotocin- (STZ-) induced DN in rats. The DN rats were treated with HSYA for eight weeks. We assessed creatinine (CR), urea nitrogen (UN), glomerular volume, podocyte number, renal inflammation, oxidative stress, and cells apoptosis markers after HSYA treatment. The number of apoptotic cells was measured by the TUNEL assay, and apoptosis-related proteins BAX, caspase-3, and BCL-2 in the renal tissue were analyzed by western blot. The treatment with HSYA significantly decreased fasting blood glucose, CR, UN, and blood lipid profile, including triglyceride and total and low-density lipoprotein cholesterol, even though it did not change the rats’ body weights. The western blot results indicated that HSYA reversed the upregulation of BAX and caspase-3 and significantly increased BCL-2 in renal tissue. Moreover, the levels of TNF-α and the inflammatory products, including free fatty acids (FFA) and lactic dehydrogenase (LDH) in the HSYA group, were significantly decreased. For the oxidative stress marker, the superoxide dismutase (SOD) markedly increased in the HSYA treatment group, while the malondialdehyde (MDA) in the serum and kidney tissue evidently decreased. In conclusion, HSYA treatment preserved kidney function in diabetic nephropathy in the HFD- and STZ-induced rats. The potential mechanism of renal protective effect of HSYA might be through inhibiting oxidative stress, reducing inflammatory reaction, and attenuating renal cell apoptosis. Our studies present a promising use for Hydroxysafflor yellow A in the treatment of type 2 diabetes mellitus.

Protective Effect of Ferulic Acid on Erythrocytes against Experimental Oxidative Stress Models

2016 ◽  
Vol 23 (1) ◽  
pp. 49
Author(s):  
Ashutosh Gupta ◽  
Aditya Ganeshpurkar ◽  
Divya Bansal ◽  
Nazneen Dubey
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Ferulic Acid ◽  
Stress Models

scholarly journals Gold nanoparticles attenuate albuminuria by inhibiting podocyte injury in a rat model of diabetic nephropathy

2019 ◽  
Vol 10 (1) ◽  
pp. 216-226 ◽  
Author(s):  
Ghada Alomari ◽  
Bahaa Al-Trad ◽  
Salehhuddin Hamdan ◽  
Alaa Aljabali ◽  
Mazhar Al-Zoubi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gold Nanoparticles ◽  
Diabetic Nephropathy ◽  
Growth Factor ◽  
Protein Expression ◽  
Male Rats ◽  
Extracellular Matrix Protein ◽  
Membrane Thickness ◽  
Podocyte Injury ◽  
Basement Membrane Thickness

Abstract Several recent studies have reported that gold nanoparticles (AuNPs) attenuate hyperglycemia in diabetic animal models without any observed side effects. The present study was intended to provide insight into the effects of 50-nm AuNPs on diabetic kidney disease. Adult male rats were divided into three groups (n = 7/group): control (non-diabetic, ND), diabetic (D), and diabetic treated intraperitoneally with 50-nm AuNPs (AuNPs + D; 2.5 mg/kg/day) for 7 weeks. Diabetes was induced by a single-dose injection of 55 mg/kg streptozotocin. The result showed that AuNP treatment prevented diabetes-associated increases in the blood glucose level. Reduction in 24-h urinary albumin excretion rate, glomerular basement membrane thickness, foot process width, and renal oxidative stress markers was also demonstrated in the AuNP-treated group. In addition, the results showed downregulation effect of AuNPs in renal mRNA or protein expression of transforming growth factor β1 (TGF-β1), fibronectin, collagen IV, tumor necrosis factor-α (TNF-α), and vascular endothelial growth factor-A (VEGF-A). Moreover, the protein expression of nephrin and podocin, podocyte markers, in glomeruli was increased in the AuNPs + D group compared with the D group. These results provide evidence that 50-nm AuNPs can ameliorate renal damage in experimental models of diabetic nephropathy through improving the renal function and downregulating extracellular matrix protein accumulation, along with inhibiting renal oxidative stress and amelioration of podocyte injury.

Valsartan slows the progression of diabetic nephropathy in db/db mice via a reduction in podocyte injury, and renal oxidative stress and inflammation

2014 ◽  
Vol 126 (10) ◽  
pp. 707-720 ◽  
Author(s):  
Guangyu Zhou ◽  
Alfred K. Cheung ◽  
Xia Liu ◽  
Yufeng Huang
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Diabetic Nephropathy ◽  
Podocyte Injury

Valsartan, given at a dose that reduces proteinuria, maximally slows the progression of the renal structural lesions resulting from Type 2 diabetes in db/db mice via protection of renal podocytes and a reduction in renal oxidative stress and inflammation.

scholarly journals Protective Effect of Keluoxin against Diabetic Nephropathy in Type 2 Diabetic Mellitus Models

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Xiaomei Yang ◽  
Xuke Han ◽  
Qing Wen ◽  
Xianliang Qiu ◽  
Huan Deng ◽  
...  
Keyword(s):  
Renal Function ◽  
Diabetic Nephropathy ◽  
Kidney Disease ◽  
Protective Effect ◽  
Blood Stasis ◽  
Histopathological Changes ◽  
Podocyte Injury ◽  
Persistent Proteinuria ◽  
Chinese Patent

Diabetic nephropathy (DN) is a chronic kidney disease that develops in patients with diabetes mellitus (DM). Renal dysfunction and persistent proteinuria are the main clinical features of DN. Podocyte injury is an important cause of persistent proteinuria and diabetic kidney disease (DKD) progression. Traditional Chinese patent medicines can improve renal function by enhancing autophagy and promoting apoptosis. Keluoxin is a Chinese patent medicine that has the effect of invigorating qi and nourishing yin, activating blood, and eliminating blood stasis. Therefore, we hypothesized that Keluoxin may have a protective effect against diabetic nephropathy in rats with type 2 DM. Rats induced with diabetes through streptozocin (STZ) injection and a high-fat and high-sugar diet were treated with Keluoxin (0.63 g/kg/day) for 8 weeks, and renal function, biochemical indicators, and histopathological changes in renal tissues were observed. Immunofluorescence staining and western blot analysis were used to detect the expression of autophagy-related proteins. The results showed that Keluoxin reduced blood glucose and lipid levels, improved renal function, and alleviated renal histopathological changes in rats with DN. The therapeutic effect was similar to that of Irbesartan (15.6 mg/kg/day). It is inferred that the mechanism works through reducing the obstruction of downstream pathways of autophagy by improving the lysosomal degradation function and alleviating podocyte injury. This study demonstrates that Keluoxin could regulate autophagy in podocytes, alleviate kidney injury in rats with DN, and have a protective effect on renal function; its mechanism can thus be a potential therapy for DN.

scholarly journals Carvedilol Ameliorates Early Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Mohamed A. Morsy ◽  
Salwa A. Ibrahim ◽  
Entesar F. Amin ◽  
Maha Y. Kamel ◽  
Soha A. Abdelwahab ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Protective Effect ◽  
Histopathological Examination ◽  
Electron Microscopic Examination ◽  
Diabetic Rats ◽  
Electron Microscopic ◽  
Podocyte Injury ◽  
Stage Renal Disease ◽  
End Stage ◽  
Early Diabetic Nephropathy

Diabetic nephropathy results in end-stage renal disease. On the other hand, carvedilol has been reported to have various pharmacological properties. The aim of this study therefore is to evaluate the possible protective effect of carvedilol on streptozotocin-induced early diabetic nephropathy and various mechanisms underlie this effect in rats. Single i.p. injection of streptozotocin (65 mg/kg) was administered to induce early diabetic nephropathy in Wistar rats. Oral administration of carvedilol at a dose level of 1 and 10 mg/kg daily for 4 weeks resulted in nephroprotective effect as evident by significant decrease in serum creatinine level, urinary albumin/creatinine ratio, and kidney index as well as renal levels of malondialdehyde, nitric oxide, tumor necrosis factor-α, and cyclooxygenase-2 with a concurrent increase in creatinine clearance and renal reduced glutathione level compared to diabetic untreated rats. The protective effect of carvedilol was confirmed by renal histopathological examination. The electron microscopic examination indicated that carvedilol could effectively ameliorate glomerular basement membrane thickening and podocyte injury. In conclusion, carvedilol protects rats against streptozotocin-induced early diabetic nephropathy possibly, in part, through its antioxidant as well as anti-inflammatory activities, and ameliorating podocyte injury.

scholarly journals Raspberry ketone improves diabetic nephropathy in mice by inhibiting streptozotocin-induced oxidative stress and inflammation

2020 ◽  
Author(s):  
Xin Wang ◽  
Ying Chang ◽  
Yan Wang ◽  
Yuxin Liu ◽  
Xiyu Hu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Nephropathy ◽  
Serum Creatinine ◽  
Protective Effect ◽  
Diagnostic Ultrasound ◽  
Expression Levels ◽  
Raspberry Ketone ◽  
Tnf Α ◽  
Diagnostic Ultrasound System ◽  
Ultrasound System

Abstract Background Oxidative stress and inflammation play important roles in the pathogenesis of diabetic nephropathy. Raspberry ketone (RK) is one of the main active substances in raspberry and has a variety of medicinal values. Nevertheless, its protective effect on streptozotocin (STZ)-induced diabetic nephropathy mice has not been reported. In this study, we researched if RK improves oxidative stress and inflammation of STZ-induced diabetic nephropathy mice. Methods The model of diabetic nephropathy mice was established by a single injection of STZ (130 mg/kg). Diabetic nephropathy mice were treated orally with 400 mg/kg/day raspberry ketone extract to eight weeks. The expression levels of serum creatinine (Scr), urine micro-albumin (mALB), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) were measured by Enzyme-linked immunosorbent (ELISA). Renal structural changes were investigated by using animal diagnostic ultrasound system. Results RK obviously ameliorated as observed in the visible decrease levels of serum creatinine (Scr), urine micro-albumin (mALB). Furthermore, RK improved expression levels of antioxidant enzyme markers (GSH-Px, SOD, CAT) and obviously reduced MDA, a product of lipid peroxidation, pro-inflammatory divisors (TNF-α, IL-6, IL-1β) in diabetic nephropathy mice. After treatment with RK, we observed that the size of the kidneys and the damage of renal interstitial of mice were obviously improved, as well as the loss of integrity of basement membrane and the renal interstitial edema in diabetic nephropathy mice by using animal diagnostic ultrasound system. Conclusion This study strongly indicates that raspberry ketone provides a protective effect against STZ-induced diabetic nephropathy in improving oxidative stress and inflammation.

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