Modulating effects of polysaccharides from the fruits ofLycium barbarumon the immune response and gut microbiota in cyclophosphamide-treated mice

2019 ◽  
Vol 10 (6) ◽  
pp. 3671-3683 ◽  
Author(s):  
Yu Ding ◽  
Yamei Yan ◽  
Dan Chen ◽  
Linwu Ran ◽  
Jia Mi ◽  
...  
Keyword(s):  
Immune Response ◽  
Gut Microbiota ◽  
Lycium Barbarum ◽  
Gut Microbiota Dysbiosis

The effects were investigated ofLycium barbarumpolysaccharides on immunoregulation and gut microbiota dysbiosis in CTX-induced mice to elucidate whether the attenuation of immunosuppression is related to the modulation of the gut microbiota.

scholarly journals Lycium barbarum relieves gut microbiota dysbiosis and improves colonic barrier function in mice following antibiotic perturbation

2020 ◽  
Vol 71 ◽  
pp. 103973 ◽  
Author(s):  
Baoming Tian ◽  
Mengyao Liu ◽  
Wei An ◽  
Lizhi Yu ◽  
Jiawei Zhang ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Barrier Function ◽  
Lycium Barbarum ◽  
Gut Microbiota Dysbiosis

scholarly journals Gut Microbiota Dysbiosis Correlates with Abnormal Immune Response in Moderate COVID-19 Patients with Fever

2021 ◽  
Vol Volume 14 ◽  
pp. 2619-2631
Author(s):  
Yaya Zhou ◽  
Xing Shi ◽  
Wei Fu ◽  
Fei Xiang ◽  
Xinliang He ◽  
...  
Keyword(s):  
Immune Response ◽  
Gut Microbiota ◽  
Abnormal Immune Response ◽  
Gut Microbiota Dysbiosis

scholarly journals Altered gut microbiota correlate with different immune response to HAART in HIV-infected individuals

2020 ◽  
Author(s):  
Yirui Xie ◽  
Jia Sun ◽  
Li Wei ◽  
Haiyin Jiang ◽  
Caiqin Hu ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cell ◽  
Gut Microbiota ◽  
Immune Activation ◽  
Highly Active Antiretroviral Therapy ◽  
Cd4 T Cell ◽  
Health Control ◽  
Cell Counts ◽  
Gut Microbiota Dysbiosis

Abstract Background: Although gut microbiota dysbiosis has recently been reported in HIV infected individuals, the relationship between the gut microbiota and immune activation in patients with a different immune response to highly active antiretroviral therapy (HAART) is still not well understood. Gut microbiota and immune activation were studied in 36 non-HIV-infected subjects (Health Control) and 58 HIV-infected individuals which included 28 immunological responders (IR) and 30 immunological non-responders (INR) (≥500 and <200 CD4+ T-cell counts/µl after 2 years of HIV-1 viral suppression respectively) without comorbidities. Results: Metagenome sequencing revealed that the gut microbiota dysbiosis could not be recovered completely in HIV-infected immunological responders and immunological non-responders although with long-term suppressive antiretroviral treatment. At the 97% similarity level, Fusobacterium, Ruminococcus_gnavus and Megamonas were more abundant, whereas Faecalibacterium, Alistipes, Bifidobacterium, Eubacteriumrectale and Roseburia were more depleted in the IR and INR groups than that in the health control. Ruminococcaceae and Alistipes were positively correlated with nadir and current CD4+ T-cell counts, but negatively correlated with CD8+CD57+ T-cell counts. Inflammation markers and translocation biomarkers (LPS) levels were positively correlated with the abundance of genus Ruminococcus and Fusobacterium, but were negatively correlated with the genus Faecalibacterium. The relative abundances of Escherichia-Shigella and Blautia were significantly higher in the IR than that in the INR group. Escherichia-Shigella were negatively correlated with the CD4/CD8 ratio but positively correlated with the amount of CD8+CD57+ T-cells. Roseburia and Blautia were negatively associated with nadir CD4+ T-cell and positively associated with CD8+CD57+ T-cell counts. Conclusions: Gut microbiota dysbiosis may be one of the factors contributing to different immune response to HAART. Fusobacterium, Alistipes, Ruminococcaceae, Faecalibacterium, Escherichia-Shigella, Roseburia and Blautia maybe the major genus contributed to different immune response in immunodiscordant and immunoconcordant patients on long-term suppressive ART. Blautia and Roseburia might be associated with treatment outcome.

scholarly journals Lactobacillus rhamnosus HDB1258 modulates gut microbiota-mediated immune response in mice with or without lipopolysaccharide-induced systemic inflammation

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sang-Kap Han ◽  
Yeon-Jeong Shin ◽  
Dong-Yeon Lee ◽  
Kyung Min Kim ◽  
Seo-Jin Yang ◽  
...  
Keyword(s):  
Immune Response ◽  
Gut Microbiota ◽  
Oral Administration ◽  
Systemic Inflammation ◽  
Nk Cell ◽  
Lactobacillus Rhamnosus ◽  
Expression Ratio ◽  
Macrophage Phagocytosis ◽  
Tnf Α ◽  
Gut Microbiota Composition

Abstract Background Gut microbiota closely communicate in the immune system to maintain a balanced immune homeostasis in the gastrointestinal tract of the host. Oral administration of probiotics modulates gut microbiota composition. In the present study, we isolated Lactobacillus rhamnosus HDB1258, which induced tumor necrosis factor (TNF)-α and interleukin (IL)-10 expression in macrophages, from the feces of breastfeeding infants and examined how HDB1258 could regulate the homeostatic immune response in mice with or without lipopolysaccharide (LPS)-induced systemic inflammation. Results Oral administration of HDB1258 significantly increased splenic NK cell cytotoxicity, peritoneal macrophage phagocytosis, splenic and colonic TNF-α expression, TNF-α to IL-10 expression ratio, and fecal IgA level in control mice, while Th1 and Treg cell differentiation was not affected in the spleen. However, HDB1258 treatment significantly suppressed peritoneal macrophage phagocytosis and blood prostaglandin E2 level in mice with LPS-induced systemic inflammation. Its treatment increased LPS-suppressed ratios of Treg to Th1 cell population, Foxp3 to T-bet expression, and IL-10 to TNF-α expression. Oral administration of HDB1258 significantly decreased LPS-induced colon shortening, myeloperoxidase activity and NF-κB+/CD11c+ cell population in the colon, while the ratio of IL-10 to TNF-α expression increased. Moreover, HDB1258 treatment shifted gut microbiota composition in mice with and without LPS-induced systemic inflammation: it increased the Cyanobacteria and PAC000664_g (belonging to Bacteroidetes) populations and reduced Deferribacteres and EU622763_s group (belonging to Bacteroidetes) populations. In particular, PAC001066_g and PAC001072_s populations were negatively correlated with the ratio of IL-10 to TNF-α expression in the colon, while the PAC001070_s group population was positively correlated. Conclusions Oral administered HDB1258 may enhance the immune response by activating innate immunity including to macrophage phagocytosis and NK cell cytotoxicity in the healthy host and suppress systemic inflammation in the host with inflammation by the modulation of gut microbiota and IL-10 to TNF-α expression ratio in immune cells.

scholarly journals Dietary switch to Western diet induces hypothalamic adaptation associated with gut microbiota dysbiosis in rats

2021 ◽  
Author(s):  
Mélanie Fouesnard ◽  
Johanna Zoppi ◽  
Mélanie Petera ◽  
Léa Le Gleau ◽  
Carole Migné ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Western Diet ◽  
Dietary Switch ◽  
Gut Microbiota Dysbiosis

scholarly journals Constipation induced gut microbiota dysbiosis exacerbates experimental autoimmune encephalomyelitis in C57BL/6 mice

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiuli Lin ◽  
Yingying Liu ◽  
Lili Ma ◽  
Xiaomeng Ma ◽  
Liping Shen ◽  
...  
Keyword(s):  
Experimental Autoimmune Encephalomyelitis ◽  
Gut Microbiota ◽  
Blood Brain Barrier ◽  
Intestinal Barrier ◽  
Brain Barrier ◽  
Autoimmune Encephalomyelitis ◽  
Gm Csf ◽  
Blood Brain ◽  
Gut Microbiota Dysbiosis ◽  
Experimental Autoimmune

Abstract Background Constipation is a common gastrointestinal dysfunction which has a potential impact on people's immune state and their quality of life. Here we investigated the effects of constipation on experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). Methods Constipation was induced by loperamide in female C57BL/6 mice. The alternations of gut microbiota, permeability of intestinal barrier and blood–brain barrier, and histopathology of colon were assessed after constipation induction. EAE was induced in the constipation mice. Fecal microbiota transplantation (FMT) was performed from constipation mice into microbiota-depleted mice. Clinical scores, histopathology of inflammation and demyelination, Treg/Th17 and Treg17/Teff17 imbalance both in the peripheral lymphatic organs and central nervous system, cytokines include TGF-β, GM-CSF, IL-10, IL-17A, IL-17F, IL-21, IL-22, and IL-23 in serum were assessed in different groups. Results Compared with the vehicle group, the constipation mice showed gut microbiota dysbiosis, colon inflammation and injury, and increased permeability of intestinal barrier and blood–brain barrier. We found that the clinical and pathological scores of the constipation EAE mice were severer than that of the EAE mice. Compared with the EAE mice, the constipation EAE mice showed reduced percentage of Treg and Treg17 cells, increased percentage of Th17 and Teff17 cells, and decreased ratio of Treg/Th17 and Treg17/Teff17 in the spleen, inguinal lymph nodes, brain, and spinal cord. Moreover, the serum levels of TGF-β, IL-10, and IL-21 were decreased while the GM-CSF, IL-17A, IL-17F, IL-22, and IL-23 were increased in the constipation EAE mice. In addition, these pathological processes could be transferred via their gut microbiota. Conclusions Our results verified that constipation induced gut microbiota dysbiosis exacerbated EAE via aggravating Treg/Th17 and Treg17/Teff17 imbalance and cytokines disturbance in C57BL/6 mice.

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