Anti-obesity effects of instant fermented teas in vitro and in mice with high-fat-diet-induced obesity

2019 ◽  
Vol 10 (6) ◽  
pp. 3502-3513 ◽  
Author(s):  
Yue Sun ◽  
Yuwan Wang ◽  
Pengpeng Song ◽  
Haisong Wang ◽  
Na Xu ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
High Fat Diet ◽  
Inhibitory Effect ◽  
High Fat ◽  
Related Factors ◽  
Diet Induced Obesity

IDT exerted better anti-obesity effects than PET in HFD-fed mice, as evidenced by reduced BW and regulated obesity-related factors. IDT also showed inhibitory effect on pancreatic lipase in vitro.

scholarly journals Glial A2B Adenosine Receptors Modulate Abnormal Tachykininergic Responses and Prevent Enteric Inflammation Associated with High Fat Diet-Induced Obesity

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1245 ◽  
Author(s):  
Vanessa D’Antongiovanni ◽  
Laura Benvenuti ◽  
Matteo Fornai ◽  
Carolina Pellegrini ◽  
Renè van den Wijngaard ◽  
...  
Keyword(s):  
Glial Cell ◽  
High Fat Diet ◽  
Enzyme Linked Immunosorbent Assay ◽  
Standard Diet ◽  
Tlr4 Expression ◽  
High Fat ◽  
Toll Like Receptor 4 ◽  
Diet Induced Obesity ◽  
Adenosine A2b

The role played by adenosine A2B receptors (A2BRs) in the regulation of enteric glial cell (EGC) functions remains unclear. This study was aimed at investigating the involvement of A2BRs in the control of EGC functions in a model of obesity. C57BL/6 mice were fed with standard diet (SD) or high fat diet (HFD) for eight weeks. Colonic tachykininergic contractions were recorded in the presence of BAY60-6583 (A2BRs agonist), MRS1754 (A2BRs antagonist), and the gliotoxin fluorocitrate. Immunofluorescence distribution of HuC/D, S100β, and A2BRs was assessed in whole mount preparations of colonic myenteric plexus. To mimic HFD, EGCs were incubated in vitro with palmitate (PA) and lipopolysaccharide (LPS), in the absence or in the presence of A2BR ligands. Toll-like receptor 4 (TLR4) expression was assessed by Western blot analysis. Interleukin-1β (IL-1β), substance P (SP), and glial cell derived neurotrophic factor (GDNF) release were determined by enzyme-linked immunosorbent assay (ELISA) assays. MRS1754 enhanced electrically evoked tachykininergic contractions of colonic preparations from HFD mice. BAY60-6583 decreased the evoked tachykininergic contractions, with higher efficacy in HFD mice. Such effects were blunted upon incubation with fluorocitrate. In in vitro experiments on EGCs, PA and LPS increased TLR4 expression as well as IL-1β, GDNF, and SP release. Incubation with BAY60-6583 reduced TLR4 expression as well as IL-1β, GDNF, and SP release. Such effects were blunted by MRS1754. The present results suggest that A2BRs, expressed on EGCs, participate in the modulation of enteric inflammation and altered tachykininergic responses associated with obesity, thus representing a potential therapeutic target.

scholarly journals Hedgehog signaling via Gli2 prevents obesity induced by high-fat diet in adult mice

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Yu Shi ◽  
Fanxin Long
Keyword(s):  
High Fat Diet ◽  
Hedgehog Signaling ◽  
Significant Risk ◽  
Whole Body ◽  
Postnatal Life ◽  
High Fat ◽  
Fat Depots ◽  
Diet Induced Obesity ◽  
Hh Signaling

Obesity poses a significant risk of developing type II diabetes and other diseases. Hedgehog (Hh) signaling has been shown to inhibit adipose tissue development, but its effect on diet-induced obesity during postnatal life is not known. Here by inducing expression of constitutively active Smoothened (SmoM2) or Gli2 (ΔNGli2) in the adipocyte lineage of postnatal mice, we show that targeted activation of Hh signaling suppresses high-fat-diet-induced obesity and improves whole-body glucose tolerance and insulin sensitivity. Both SmoM2 and ΔNGli2 induce the expression of Wnt6, a known anti-adipogenic factor, in fat depots of the mouse. Hh-Gli2 signaling inhibits not only adipocyte differentiation but also lipogenesis in adipocytes in vitro. Finally, pharmacological inhibition of Porcupine, an acyltransferase essential for Wnt secretion, alleviates both anti-adipogenic and anti-lipogenic effects of Hh in cell culture models. Overall, targeted activation of Hh signaling ameliorates diet-induced obesity and may be explored for pharmaceutical development.

scholarly journals The Resin fromProtium heptaphyllumPrevents High-Fat Diet-Induced Obesity in Mice: Scientific Evidence and Potential Mechanisms

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Karine Maria Martins Bezerra Carvalho ◽  
José Delano Barreto Marinho Filho ◽  
Tiago Sousa de Melo ◽  
Ana Jérsia Araújo ◽  
Josiane da Silva Quetz ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Scientific Evidence ◽  
Net Energy ◽  
High Fat ◽  
Beneficial Effects ◽  
Proinflammatory Mediators ◽  
Glucose And Lipid Metabolism ◽  
Diet Induced Obesity ◽  
Obesity In Mice

Herbal compounds rich in triterpenes are well known to regulate glucose and lipid metabolism and to have beneficial effects on metabolic disorders. The present study investigated the antiobesity properties of resin fromProtium heptaphyllum(RPH) and the possible mechanisms in mice fed a high-fat diet (HFD) for 15 weeks. Mice treated with RPH showed decreases in body weight, net energy intake, abdominal fat accumulation, plasma glucose, amylase, lipase, triglycerides, and total cholesterol relative to their respective controls, which were RPH unfed. Additionally, RPH treatment, while significantly elevating the plasma level of ghrelin hormone, decreased the levels of insulin, leptin, and resistin. Besides, HFD-induced increases in plasma levels of proinflammatory mediators TNF-α, IL-6, and MCP-1 were significantly lowered by RPH. Furthermore,in vitrostudies revealed that RPH could significantly inhibit the lipid accumulation in 3T3-L1 adipocytes (measured by Oil-Red O staining) at concentrations up to 50 μg/mL. These findings suggest that the antiobese potential of RPH is largely due to its modulatory effects on various hormonal and enzymatic secretions related to fat and carbohydrate metabolism and to the regulation of obesity-associated inflammation.

scholarly journals Maté Tea Inhibits In Vitro Pancreatic Lipase Activity and Has Hypolipidemic Effect on High-fat Diet-induced Obese Mice

Obesity ◽  
2010 ◽  
Vol 18 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Fernanda Martins ◽  
Tatiana M. Noso ◽  
Viviane B. Porto ◽  
Alline Curiel ◽  
Alessandra Gambero ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
High Fat Diet ◽  
Hypolipidemic Effect ◽  
Obese Mice ◽  
High Fat ◽  
Mate Tea

scholarly journals Effect of Aruncus dioicus var. kamtschaticus Extract on Neurodegeneration Improvement: Ameliorating Role in Cognitive Disorder Caused by High-Fat Diet Induced Obesity

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1319 ◽  
Author(s):  
Su Bin Park ◽  
Jin Yong Kang ◽  
Jong Min Kim ◽  
Seon Kyeong Park ◽  
Seul Ki Yoo ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Brain Tissue ◽  
High Fat Diet ◽  
Cognitive Disorder ◽  
High Fat ◽  
Related Factors ◽  
Necrosis Factor Alpha ◽  
Peripheral Tissues

This study was performed to estimate the possibility of using an ethyl acetate fraction from Aruncus dioicus var. kamtschaticus (EFAD) on metabolic syndrome that is induced by a high-fat diet (HFD). It was demonstrated that EFAD suppresses lipid accumulation and improves insulin resistance (IR) caused by Tumor necrosis factor alpha (TNF-α) in in-vitro experiments using the 3T3-L1 cell. In in-vivo tests, C57BL/6 mice were fed EFAD at 20 and 40 mg/kg body weight (BW) for four weeks after the mice were fed HFD for 15 weeks to induce obesity. EFAD significantly suppressed the elevation of BW and improved impaired glucose tolerance in obese mice. Additionally, this study showed that EFAD has an ameliorating effect on obesity-induced cognitive disorder with behavioral tests. The effect of EFAD on peripheral-IR improvement was confirmed by serum analysis and western blotting in peripheral tissues. Additionally, EFAD showed an ameliorating effect on HFD-induced oxidative stress, impaired cholinergic system and mitochondrial dysfunction, which are interrelated symptoms of neurodegeneration, such as Alzheimer’s disease and central nervous system (CNS)-IR in brain tissue. Furthermore, we confirmed that EFAD improves CNS-IR by confirming the IR-related factors in brain tissue. Consequently, this study suggests the possibility of using EFAD for the prevention of neurodegeneration by improving metabolic syndrome that is caused by HFD.

scholarly journals Camellia sinensis extract inhibits in vitro pancreatic lipase and has preventive effect on obesity in female rat fed a high-fat diet

2015 ◽  
Vol 9 (37) ◽  
pp. 919-928
Author(s):  
Carolina Vieira Almeida Erika ◽  
Antonio Pigoso Ac aacute cio ◽  
Fernando de Almeida Alex
Keyword(s):  
Camellia Sinensis ◽  
Pancreatic Lipase ◽  
High Fat Diet ◽  
Female Rat ◽  
Preventive Effect ◽  
High Fat

scholarly journals Evaluation of anti-obesity potential of aqueous extract of Achyranthes aspera Linn. in high fat diet induced obese rats

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Kumaraswamy Athesh ◽  
Rangaraju Sivasubramanian ◽  
Gnanasekaran Jothi ◽  
Pemiah Brindha
Keyword(s):  
Aqueous Extract ◽  
Pancreatic Lipase ◽  
High Fat Diet ◽  
In Vivo Studies ◽  
Control Group ◽  
High Fat ◽  
Achyranthes Aspera ◽  
Obese Rats

Abstract Background Obesity, reached epidemic proportions globally is often associated with life threatening comorbidities. The unavailability of safe and effective long term medications for obesity in modern pharmacotherapy forces the scientific community to explore the potential of Ayurvedic traditional healers as they are considered safe and effective. Objective To explore the anti-obesity potential of aqueous extract of aerial parts of Achyranthes aspera L. (AEAA), a traditional healer in high fat diet (HFD) induced obese rats. Methods AEAA was prepared and subjected to in-vitro pancreatic lipase inhibition assay and in-vivo anti-obesity studies. For in-vivo studies, HFD fed obese prone Wistar albino rats were divided into five experimental groups (Group II to VI): animals fed with standard pellet chow served as normal control (Group I) while, animals continued with HFD alone served as obese control (Group II); Group III, IV and V were administered AEAA at a dose of 100, 200 and 300 mg/kg b.w. respectively along with HFD; and animals administered orlistat (30 mg/kg bw) along with HFD served as standard control (Group VI). All the drugs were administered orally once a day for a period of 60 days. At the end of the experimental period various physical, biochemical and histopathological observations were made. Results In-vitro studies showed AEAA partially but not significantly inhibited the activity of pancreatic lipase. Data of in-vivo studies revealed, significant reduction in body weights, fat pad weights and organ weights upon AEAA treatment. Elevated levels of glucose, insulin, leptin, lipid profiles and antioxidant status were also brought back to normal. Conclusion The obtained results clearly suggested that AEAA possess pronounced anti-obesity potential.

Comparative effects of piperine and simvastatin in fat accumulation and antioxidative status in high fat-induced hyperlipidemic rats

2016 ◽  
Vol 94 (12) ◽  
pp. 1344-1348 ◽  
Author(s):  
Sakara Tunsophon ◽  
Krongkarn Chootip
Keyword(s):  
Pancreatic Lipase ◽  
In Vitro Study ◽  
Inhibitory Effect ◽  
Liver Cholesterol ◽  
High Fat ◽  
Serum Aminotransferase ◽  
Fat Accumulation ◽  
Antioxidative Status ◽  
Beneficial Effects

The present study investigated the comparative effects of piperine (PIP) — the active ingredient of black and long peppers — and simvastatin (SIM) on hepatic steatosis in hyperlipidemic rats. Male Wistar rats were fed a cholesterol mixture daily by intragastric gavage for 8 weeks. Piperine was given by oral gavage 8 h after cholesterol feeding. The animals were divided into 4 groups: control, high fat (HF), high fat plus 40 mg PIP/kg, and high fat plus 2 mg SIM/kg. At the end of the treatment, liver cholesterol, triglyceride, thiobaribituric reacting substances, superoxide dismutase (SOD), serum aminotransferase (AST), and alanine transferase (ALT) were measured. The result demonstrated that PIP and SIM significantly reduced the accumulation of cholesterol, triglyceride, and lipid peroxidation in the liver, while elevation of SOD was observed. The activities of AST and ALT significantly decreased in PIP when compared with the HF group. Our in vitro study of pancreatic lipase also showed the inhibitory effect of PIP higher than 30% at 5 mmol/L. These results demonstrate that PIP has beneficial effects in the treatment and (or) prevention of fat accumulation in the liver and that this mechanism is due to the inhibition of pancreatic lipase and the improvement of oxidative status.

scholarly journals Inhibitory Effects of Six Types of Tea on Aging and High-Fat Diet-Related Amyloid Formation Activities

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1513
Author(s):  
Juan Wan ◽  
Meiyan Feng ◽  
Wenjing Pan ◽  
Xin Zheng ◽  
Xinya Xie ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
High Fat Diet ◽  
Inhibitory Effect ◽  
Premature Aging ◽  
High Fat ◽  
Amyloid A ◽  
Significant Inhibitory Effect ◽  
Lipid Metabolism Disorders ◽  
Age Related

Aging and lipid metabolism disorders promote the formation and accumulation of amyloid with β-sheet structure, closely related to cardiovascular disease, senile dementia, type 2 diabetes, and other senile degenerative diseases. In this study, five representative teas were selected from each of the six types of tea, and a total of 30 teas were selected to evaluate the inhibitory activities on the formation of aging-related amyloid in vitro. The results showed that the 30 teas had a significant inhibitory effect on the formation activity on aging-related amyloid at the protein level in vitro. Although the content of catechins is relatively low, black tea and dark tea still have significant antioxidant activity and inhibit the formation of amyloid. A high-fat diet established the model of lipid metabolism disorder in premature aging SAMP8 mice, and these mice were gavaged different tea water extracts. The results showed that different tea types have a significant inhibitory effect on the formation of β-amyloid and Aβ42 mediated by age-related lipid metabolism disorders, and the in vivo activity of fully fermented teas was better than that of green tea. The action mechanism was related to antioxidation, anti-inflammatory, and improving lipid metabolism.

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