scholarly journals Adaptation of Pseudomonas aeruginosa to constant sub-inhibitory concentrations of quaternary ammonium compounds

2020 ◽  
Vol 6 (4) ◽  
pp. 1139-1152 ◽  
Author(s):  
Margaux Voumard ◽  
Leonardo Venturelli ◽  
Myriam Borgatta ◽  
Antony Croxatto ◽  
Sandor Kasas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quaternary Ammonium ◽  
Quaternary Ammonium Compounds ◽  
Membrane Properties ◽  
Ammonium Compounds

The susceptibility profile of P. aeruginosa exposed to constant sub-inhibitory 3 concentrations of quaternary ammonium compounds was characterized as well as the 4 nanomechanical membrane properties.

A COMPARISON OF SOME PROPERTIES OF STRAINS OF PSEUDOMONAS AERUGINOSA SENSITIVE AND RESISTANT TO QUATERNARY AMMONIUM COMPOUNDS

1958 ◽  
Vol 4 (5) ◽  
pp. 499-503 ◽  
Author(s):  
D. R. MacGregor ◽  
P. R. Elliker
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ammonium Chloride ◽  
Yeast Extract ◽  
Quaternary Ammonium ◽  
Substrate Oxidation ◽  
Quaternary Ammonium Compounds ◽  
Extract Agar ◽  
Resistant Strains ◽  
Ammonium Compounds ◽  
Yeast Extract Agar

Pseudomonas aeruginosa was adapted to grow on tryptone – glucose – yeast extract agar containing 2000 p.p.m. alkyldimethylethylbenzyl ammonium chloride (QAC-A). In germicidal trials these resistant cells could be made QAC-A sensitive by treatment with ethylenediaminetetraacetate. In Warburg studies lyophilized preparations of resistant strains were more sensitive to inhibition of substrate oxidation by QAC-A than the normal strain.It is postulated that quaternary ammonium germicide resistant strains of P. aeruginosa owe their resistance to their impermeability to QAC.

scholarly journals Correlation between Resistance of Pseudomonas aeruginosa to Quaternary Ammonium Compounds and Expression of Outer Membrane Protein OprR

2003 ◽  
Vol 47 (7) ◽  
pp. 2093-2099 ◽  
Author(s):  
Atsushi Tabata ◽  
Hideaki Nagamune ◽  
Takuya Maeda ◽  
Keiji Murakami ◽  
Yoichiro Miyake ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Quaternary Ammonium ◽  
Resistant Strain ◽  
Quaternary Ammonium Compounds ◽  
Cross Resistance ◽  
Knockout Mutant ◽  
Ammonium Compounds

ABSTRACT The adaptation mechanism of Pseudomonas aeruginosa ATCC 10145 to quaternary ammonium compounds (QACs) was investigated. A P. aeruginosa strain with adapted resistance to QACs was developed by a standard broth dilution method. It was revealed that P. aeruginosa exhibited remarkable resistance to N-dodecylpyridinium iodide (P-12), whose structure is similar to that of a common disinfectant, cetylpyridinium chloride. Adapted resistance to benzalkonium chloride (BAC), which is commonly used as a disinfectant, was also observed in P. aeruginosa. Moreover, the P-12-resistant strain exhibited cross-resistance to BAC. Analysis of the outer membrane protein of the P-12-resistant strain by two-dimensional polyacrylamide gel electrophoresis showed a significant increase in the level of expression of a protein (named OprR) whose molecular mass was approximately 26 kDa. The actual function of OprR is not yet clear; however, OprR was expected to be an outer membrane-associated protein with homology to lipoproteins of other bacterial species, according to a search of the National Center for Biotechnology Information website with the BLAST program by use of the N-terminal sequence of OprR. A correlation between the level of expression of OprR and the level of resistance of P. aeruginosa to QACs was observed by using a PA2800 gene knockout mutant derived from the P-12-resistant strain. The knockout mutant recovered susceptibility not only to P-12 but also to BAC. These results suggested that OprR significantly participated in the adaptation of P. aeruginosa to QACs, such as P-12 and BAC.

Determination of quaternary ammonium compounds in food products by the method of ultra-performance liquid chromatography/high resolution mass-spectrometry

2020 ◽  
Vol 86 (8) ◽  
pp. 23-31
Author(s):  
V. G. Amelin ◽  
D. S. Bolshakov
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Quaternary Ammonium ◽  
High Resolution Mass Spectrometry ◽  
Food Products ◽  
Quaternary Ammonium Compounds ◽  
High Resolution Mass ◽  
Ammonium Compounds ◽  
Resolution Mass

The goal of the study is developing a methodology for determination of the residual amounts of quaternary ammonium compounds (QAC) in food products by UHPLC/high-resolution mass spectrometry after water-acetonitrile extraction of the determined components from the analyzed samples. The identification and determination of QAC was carried out on an «UltiMate 3000» ultra-high-performance liquid chromatograph (Thermo Scientific, USA) equipped with a «maXis 4G» high-resolution quadrupole-time-of-flight mass spectrometric detector and an ion spray «ionBooster» source (Bruker Daltonics, Germany). Samples of milk, cheese (upper cortical layer), dumplings, pork, chicken skin and ground beef were used as working samples. Optimal conditions are specified for chromatographic separation of the mixture of five QAC, two of them being a mixture of homologues with a linear structure (including isomeric forms). The identification of QAC is carried out by the retention time, exact mass of the ions, and coincidence of the mSigma isotopic distribution. The limits for QAC detection are 0.1 – 0.5 ng/ml, the determination limits are 1 ng/ml for aqueous standard solutions. The determinable content of QAC in food products ranges within 1 – 100 ng/g. The results of analysis revealed the residual amount of QAC present in all samples, which confirms data of numerous sources of information about active use of QAC-based disinfectants in the meat and dairy industry. The correctness of the obtained results is verified by introduction of the additives in food products at a level of 10 ng/g for each QAC. The relative standard deviation of the analysis results does not exceed 0.18. The duration of the analysis is 30 – 40 min.

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