scholarly journals Quantitative measures of myo-IP6 in soil using solution 31P NMR spectroscopy and spectral deconvolution fitting including a broad signal

2020 ◽  
Vol 22 (4) ◽  
pp. 1084-1094 ◽  
Author(s):  
Jolanda E. Reusser ◽  
René Verel ◽  
Emmanuel Frossard ◽  
Timothy I. McLaren
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
Inositol Phosphates ◽  
Organic Phosphorus ◽  
Terrestrial Ecosystems ◽  
31P Nmr Spectroscopy ◽  
Inositol Hexakisphosphate ◽  
Soil Organic Phosphorus ◽  
Broad Signal ◽  
Solution 31P Nmr Spectroscopy

Inositol phosphates, particularly myo-inositol hexakisphosphate (myo-IP6), are an important pool of soil organic phosphorus (P) in terrestrial ecosystems.

scholarly journals Identification of lower-order inositol phosphates (IP<sub>5</sub> and IP<sub>4</sub>) in soil extracts as determined by hypobromite oxidation and solution <sup>31</sup>P NMR spectroscopy

2019 ◽  
Author(s):  
Jolanda E. Reusser ◽  
René Verel ◽  
Daniel Zindel ◽  
Emmanuel Frossard ◽  
Timothy I. McLaren
Keyword(s):  
Nmr Spectroscopy ◽  
Lower Order ◽  
31P Nmr ◽  
Inositol Phosphates ◽  
31P Nmr Spectroscopy ◽  
Organic P ◽  
Soil Extracts ◽  
Broad Signal ◽  
Solution 31P Nmr Spectroscopy ◽  
First Time

Abstract. Inositol phosphates (IP) are a major pool of identifiable organic phosphorus (P) in soil. However, insight on their distribution and cycling in soil remains limited, particularly of lower-order IP (IP5 and IP4). This is because their quantification typically requires a series of chemical extractions, including hypobromite oxidation to isolate IP, followed by chromatographic separation. Here, for the first time, we identify the chemical nature of organic P in four soil extracts following hypobromite oxidation using solution 31P NMR spectroscopy and transverse relaxation (T2) experiments. Soil samples analysed include the A horizon of a Ferralsol from Colombia, of a Cambisol from Switzerland, of a Gleysol from Switzerland and of a Cambisol from Germany. Solution 31P NMR spectra of the phosphomonoester region on soil extracts following hypobromite oxidation revealed an increase in the number of sharp signals (up to 70), and an on average 2-fold decrease in the concentration of the broad signal compared to the untreated soil extracts. We identified the presence of four stereoisomers of IP6, four stereoisomers of IP5, and scyllo-IP4 (using solution 31P NMR spectroscopy). We also identified for the first time two isomers of myo-IP5 in soil extracts: myo-(1,2,4,5,6)-IP5 and myo-(1,3,4,5,6)-IP5. Concentrations of total IP ranged from 1.4 to 159.3 mg P/kgsoil across all soils, of which between 9 % and 50 % were comprised of lower-order IP. Furthermore, we found that the T2 times, which are considered to be inversely related to the tumbling of a molecule in solution and hence its molecular size, were significantly shorter for the underlying broad signal compared to the sharp signals (IP6) in soil extracts following hypobromite oxidation. In summary, we demonstrate the presence of a plethora of organic P compounds in soil extracts, largely attributed to IP of various order, and provide new insight on the chemical stability of complex forms of organic P associated with soil organic matter.

scholarly journals Determination of neo- and d-chiro-Inositol Hexakisphosphate in Soils by Solution 31P NMR Spectroscopy

2012 ◽  
Vol 46 (9) ◽  
pp. 4994-5002 ◽  
Author(s):  
Benjamin L. Turner ◽  
Alexander W. Cheesman ◽  
H. Yasmin Godage ◽  
Andrew M. Riley ◽  
Barry V. L. Potter
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
31P Nmr Spectroscopy ◽  
Inositol Hexakisphosphate ◽  
Solution 31P Nmr Spectroscopy

31P NMR spectroscopy and structural models of soil organic phosphorus under Eucalyptus

2021 ◽  
Author(s):  
Laís Chierici Bernardes Rinaldi ◽  
Seldon Aleixo ◽  
Erika Caitano da Silva ◽  
Antônio Carlos Gama-Rodrigues ◽  
Emanuela Forestieri Gama-Rodrigues ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
Organic Phosphorus ◽  
Structural Models ◽  
31P Nmr Spectroscopy ◽  
Soil Organic Phosphorus

scholarly journals Identification of lower-order inositol phosphates (IP<sub>5</sub> and IP<sub>4</sub>) in soil extracts as determined by hypobromite oxidation and solution <sup>31</sup>P NMR spectroscopy

2020 ◽  
Vol 17 (20) ◽  
pp. 5079-5095
Author(s):  
Jolanda E. Reusser ◽  
René Verel ◽  
Daniel Zindel ◽  
Emmanuel Frossard ◽  
Timothy I. McLaren
Keyword(s):  
Nmr Spectroscopy ◽  
Lower Order ◽  
Inositol Phosphates ◽  
Organic Phosphorus ◽  
31P Nmr Spectroscopy ◽  
Organic P ◽  
Soil Extracts ◽  
Broad Signal ◽  
First Time ◽  
Insight Into

Abstract. Inositol phosphates (IPs) are a major pool of identifiable organic phosphorus (P) in soil. However, insight into their distribution and cycling in soil remains limited, particularly of lower-order IP (IP5 and IP4). This is because the quantification of lower-order IP typically requires a series of chemical extractions, including hypobromite oxidation to isolate IP, followed by chromatographic separation. Here, for the first time, we identify the chemical nature of organic P in four soil extracts following hypobromite oxidation using solution 31P NMR spectroscopy and transverse relaxation (T2) experiments. Soil samples analysed include A horizons from a Ferralsol (Colombia), a Cambisol and a Gleysol from Switzerland, and a Cambisol from Germany. Solution 31P nuclear magnetic resonance (NMR) spectra of the phosphomonoester region in soil extracts following hypobromite oxidation revealed an increase in the number of sharp signals (up to 70) and an on average 2-fold decrease in the concentration of the broad signal compared to the untreated soil extracts. We identified the presence of four stereoisomers of IP6, four stereoisomers of IP5, and scyllo-IP4. We also identified for the first time two isomers of myo-IP5 in soil extracts: myo-(1,2,4,5,6)-IP5 and myo-(1,3,4,5,6)-IP5. Concentrations of total IP ranged from 1.4 to 159.3 mg P per kg soil across all soils, of which between 9 % and 50 % were comprised of lower-order IP. Furthermore, we found that the T2 times, which are considered to be inversely related to the tumbling of a molecule in solution and hence its molecular size, were significantly shorter for the underlying broad signal compared to for the sharp signals (IP6) in soil extracts following hypobromite oxidation. In summary, we demonstrate the presence of a plethora of organic P compounds in soil extracts, largely attributed to IPs of various orders, and provide new insight into the chemical stability of complex forms of organic P associated with soil organic matter.

Response to Comment on “Determination of neo- and d-chiro-Inositol Hexakisphosphate in Soils by Solution 31P NMR Spectroscopy”

2012 ◽  
Vol 46 (20) ◽  
pp. 11480-11481 ◽  
Author(s):  
Benjamin L. Turner ◽  
Alexander W. Cheesman ◽  
H. Yasmin Godage ◽  
Andrew M. Riley ◽  
Barry V. L. Potter
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
31P Nmr Spectroscopy ◽  
Inositol Hexakisphosphate ◽  
Solution 31P Nmr Spectroscopy

Characterisation of soil organic phosphorus in NaOH-EDTA extracts: A comparison of 31P NMR spectroscopy and enzyme addition assays

2015 ◽  
Vol 91 ◽  
pp. 298-309 ◽  
Author(s):  
Klaus A. Jarosch ◽  
Ashlea L. Doolette ◽  
Ronald J. Smernik ◽  
Federica Tamburini ◽  
Emmanuel Frossard ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
31P Nmr ◽  
Organic Phosphorus ◽  
31P Nmr Spectroscopy ◽  
Soil Organic Phosphorus
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