scholarly journals Implicit solvent effects in the determination of Brønsted–Evans–Polanyi relationships for heterogeneously catalyzed reactions

2019 ◽  
Vol 21 (32) ◽  
pp. 17687-17695 ◽  
Author(s):  
José R. B. Gomes ◽  
Francesc Viñes ◽  
Francesc Illas ◽  
José L. C. Fajín
Keyword(s):  
Solvent Effects ◽  
Water Dissociation ◽  
Implicit Solvent ◽  
Dissociation Reaction ◽  
Catalyzed Reactions

The Brønsted–Evans–Polanyi relationship derived for the water dissociation reaction within an implicit solvent approach is similar to that without such effects.

Nonproteolyzed Form of DNA-Polymerase ε from T-Cell Spontaneous Lymphoma of Sprague-Dawley Inbred Rat: Isolation and Characterization

1998 ◽  
Vol 63 (5) ◽  
pp. 723-731 ◽  
Author(s):  
Gabriel Birkuš ◽  
Pavel Kramata ◽  
Ivan Votruba ◽  
Berta Otová ◽  
Miroslav Otmar ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Sedimentation Coefficient ◽  
Isolation Procedure ◽  
Sprague Dawley ◽  
Nucleotide Analogs ◽  
Isolation And Characterization ◽  
Inbred Rats ◽  
Catalyzed Reactions ◽  
Inbred Rat

Using a simple isolation procedure and selective assay for the determination of enzyme activity the nonproteolyzed and proteolyzed form of DNA-polymerase ε (pol ε and pol ε*) from the lymphoma of Sprague-Dawley inbred rats were purified. Nonproteolyzed pol ε is composed of two subunits (240 000 and 50 000) with sedimentation coefficient 10.5 S, while the subunit composition of pol ε* was 145 000 and 73 000. Estimated Km values for dATP and dGTP as well as Ki values for acyclic nucleotide analogs (PMEApp, HPMPApp and PMEDAPpp) in pol ε and pol ε* catalyzed reactions have shown that a proteolysis probably does not affect pol ε binding site for dNTPs. Both enzymes (pol ε and pol ε*) possess 3'-5'-exonuclease activity with different Km for 3'-OH end of template poly dA-oligo dT18 (1.6 μmol/l and 0.36 μmol/l, respectively).

scholarly journals Isomerization of an enzyme-coenzyme complex in yeast alcohol dehydrogenase-catalysed reactions

2003 ◽  
Vol 68 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Vladimir Leskovac ◽  
Svetlana Trivic ◽  
Draginja Pericin
Keyword(s):  
Alcohol Dehydrogenase ◽  
Rate Constants ◽  
Equilibrium Constants ◽  
Kinetic Mechanism ◽  
Yeast Alcohol Dehydrogenase ◽  
Catalyzed Reactions ◽  
The Individual ◽  
Individual Rate ◽  
Catalyzed Oxidation

In this work, all the rate constants in the kinetic mechanism of the yeast alcohol dehydrogenase-catalyzed oxidation of ethanol by NAD+, at pH 7.0, 25 ?C, have been estimated. The determination of the individual rate constants was achieved by fitting the reaction progress curves to the experimental data, using the procedures of the FITSIM and KINSIM software package of Carl Frieden. This work is the first report in the literature showing the internal equilibrium constants for the isomerization of the enzyme-NAD+ complex in yeast alcohol dehydrogenase-catalyzed reactions.

Enzymic colorimetric determination of phosphatidylglycerol in amniotic fluid.

1984 ◽  
Vol 30 (4) ◽  
pp. 534-537 ◽  
Author(s):  
J D Artiss ◽  
M W McGowan ◽  
D R Strandbergh ◽  
E Epstein ◽  
B Zak
Keyword(s):  
Amniotic Fluid ◽  
Fetal Lung ◽  
Aqueous Sample ◽  
Colorimetric Determination ◽  
Glycerol Phosphate ◽  
Ionic Detergent ◽  
Catalyzed Reactions ◽  
Enzyme Catalyzed Reactions ◽  
Hydrolysis Of

Abstract We describe a procedure for the enzymic, colorimetric determination of phosphatidylglycerol in amniotic fluid. After extraction into chloroform:methanol (2:1 by vol) and evaporation, the phospholipid-containing residue is redissolved in a non-ionic detergent, which thus provides an aqueous sample. The subsequent enzymic reaction sequence involves phospholipase-catalyzed hydrolysis of glycerol from its phospholipid. Subsequent enzyme-catalyzed reactions phosphorylate this glycerol and oxidize the resulting glycerol phosphate to produce hydrogen peroxide, which is reacted to produce an intense red chromogen in the peroxidase-catalyzed coupling of 4-aminoantipyrine and 2-hydroxy-3,5-dichlorobenzenesulfonate. When used in conjunction with previously reported enzymic techniques for determination of lecithin and sphingomyelin, this procedure may provide an accurate and precise "lung profile" for assessment of fetal lung maturity.

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