Computational modeling of the catalytic mechanism of hydroxymethylbilane synthase

2019 ◽  
Vol 21 (15) ◽  
pp. 7932-7940 ◽  
Author(s):  
Navneet Bung ◽  
Arijit Roy ◽  
U. Deva Priyakumar ◽  
Gopalakrishnan Bulusu
Keyword(s):  
Computational Modeling ◽  
Catalytic Mechanism ◽  
Heme Biosynthesis ◽  
Biosynthesis Pathway ◽  
The Third

Hydroxymethylbilane synthase (HMBS), the third enzyme in the heme biosynthesis pathway, catalyzes the formation of 1-hydroxymethylbilane (HMB) by a stepwise polymerization of four molecules of porphobilinogen (PBG) using the dipyrromethane (DPM) cofactor.

scholarly journals NFE2 regulates transcription of multiple enzymes in the heme biosynthesis pathway

Haematologica ◽  
2014 ◽  
Vol 99 (10) ◽  
pp. e208-e210 ◽  
Author(s):  
L. Rheinemann ◽  
T. S. Seeger ◽  
J. Wehrle ◽  
H. L. Pahl
Keyword(s):  
Heme Biosynthesis ◽  
Biosynthesis Pathway

scholarly journals Laboratory Diagnosis of Porphyria

Diagnostics ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1343
Author(s):  
Elena Di Pierro ◽  
Michele De Canio ◽  
Rosa Mercadante ◽  
Maria Savino ◽  
Francesca Granata ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Diagnostic Testing ◽  
Laboratory Diagnosis ◽  
Clinical Suspicion ◽  
Enzymatic Activities ◽  
Heme Biosynthesis ◽  
Present Review ◽  
Biosynthesis Pathway ◽  
Clinical Presentations

Porphyrias are a group of diseases that are clinically and genetically heterogeneous and originate mostly from inherited dysfunctions of specific enzymes involved in heme biosynthesis. Such dysfunctions result in the excessive production and excretion of the intermediates of the heme biosynthesis pathway in the blood, urine, or feces, and these intermediates are responsible for specific clinical presentations. Porphyrias continue to be underdiagnosed, although laboratory diagnosis based on the measurement of metabolites could be utilized to support clinical suspicion in all symptomatic patients. Moreover, the measurement of enzymatic activities along with a molecular analysis may confirm the diagnosis and are, therefore, crucial for identifying pre-symptomatic carriers. The present review provides an overview of the laboratory assays used most commonly for establishing the diagnosis of porphyria. This would assist the clinicians in prescribing appropriate diagnostic testing and interpreting the testing results.

scholarly journals Toxoplasma gondii requires its plant-like heme biosynthesis pathway for infection

2020 ◽  
Vol 16 (5) ◽  
pp. e1008499
Author(s):  
Amy Bergmann ◽  
Katherine Floyd ◽  
Melanie Key ◽  
Carly Dameron ◽  
Kerrick C. Rees ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Heme Biosynthesis ◽  
Biosynthesis Pathway

scholarly journals The essential role of the transporter ABCG2 in the pathophysiology of erythropoietic protoporphyria

2019 ◽  
Vol 5 (9) ◽  
pp. eaaw6127 ◽  
Author(s):  
Pengcheng Wang ◽  
Madhav Sachar ◽  
Jie Lu ◽  
Amina I. Shehu ◽  
Junjie Zhu ◽  
...  
Keyword(s):  
Essential Role ◽  
Protoporphyrin Ix ◽  
Heme Biosynthesis ◽  
Biosynthesis Pathway ◽  
Efflux Transporter ◽  
Inherited Disease ◽  
Loss Of Function ◽  
Erythropoietic Protoporphyria

Erythropoietic protoporphyria (EPP) is an inherited disease caused by loss-of-function mutations of ferrochelatase, an enzyme in the heme biosynthesis pathway that converts protoporphyrin IX (PPIX) into heme. PPIX accumulation in patients with EPP leads to phototoxicity and hepatotoxicity, and there is no cure. Here, we demonstrated that the PPIX efflux transporter ABCG2 (also called BCRP) determines EPP-associated phototoxicity and hepatotoxicity. We found that ABCG2 deficiency decreases PPIX distribution to the skin and therefore prevents EPP-associated phototoxicity. We also found that ABCG2 deficiency protects against EPP-associated hepatotoxicity by modulating PPIX distribution, metabolism, and excretion. In summary, our work has uncovered an essential role of ABCG2 in the pathophysiology of EPP, which suggests the potential for novel strategies in the development of therapy for EPP.

Heme biosynthesis pathway regulation in a model of hepatocarcinogenesis pre-initiation

1992 ◽  
Vol 103 (1) ◽  
pp. 251-256 ◽  
Author(s):  
César F. Polo ◽  
Elba S. Vazquez ◽  
Fabiana Caballero ◽  
Esther Gerez ◽  
Alcira M.del C. Batlle
Keyword(s):  
Heme Biosynthesis ◽  
Biosynthesis Pathway ◽  
Pathway Regulation

scholarly journals A PEER REVIEWED ONLINE COMPUTATIONAL MODELING FRAMEWORK

2011 ◽  
Author(s):  
Pieter J. Mosterman ◽  
Don Bouldin ◽  
Andrzej Rucinski
Keyword(s):  
Intellectual Property ◽  
Computational Modeling ◽  
Scientific Discovery ◽  
Intrinsic Value ◽  
Computational Simulation ◽  
Modeling Framework ◽  
Quality Requirements ◽  
The Third ◽  
Model Based

Along with theory and experimentation, computational simulation has become the third pillar of scientific discovery. While in industry computational modeling has seen application at an enterprise-wide level in the context of Model-Based Design, in academia models are typically still limited to isolated use by specialists. Once a project is completed, the intellectual property embodied by the model is lost. To harness the effort spent, a networked repository is proposed that stores peer-reviewed models. These models are evaluated whether they adhere to a set of quality requirements so they capture intrinsic value. This would facilitate the type of multi-disciplinary collaboration that is required to engineer the systems that have emerged and that continue to gain in importance. This work puts forward an outline of such a peer-reviewed online repository.

Sign in / Sign up

Export Citation Format

Share Document