scholarly journals In situ flow cell platform for examining calcium oxalate and calcium phosphate crystallization on films of basement membrane extract in the presence of urinary ‘inhibitors’

CrystEngComm ◽  
2020 ◽  
Vol 22 (8) ◽  
pp. 1448-1458 ◽  
Author(s):  
Cary A. Kuliasha ◽  
Douglas Rodriguez ◽  
Archana Lovett ◽  
Laurie B. Gower
Keyword(s):  
Calcium Phosphate ◽  
Basement Membrane ◽  
Calcium Oxalate ◽  
Flow Cell ◽  
Biologically Relevant ◽  
Basement Membrane Extract ◽  
Urinary Inhibitors ◽  
Membrane Extract

The development of a flow-cell platform is reported that utilizes basement membrane extract as a biologically relevant crystallization substrate to study the effect of urinary inhibitors on the in situ formation of calcium oxalate/phosphate.

Cardiac myocytes cultured on a basement membrane extract of the ehs tumor

1986 ◽  
Vol 44 ◽  
pp. 270-271
Author(s):  
L. Terracio ◽  
A. Dewey ◽  
K. Rubin ◽  
T.K. Borg
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Cardiac Myocytes ◽  
Normal Tissue ◽  
Cell Spreading ◽  
Collagen Iv ◽  
Basement Membrane Extract ◽  
Membrane Extract ◽  
Growth Development ◽  
Multicellular Organisms

The recognition and interaction of cells with the extracellular matrix (ECM) effects the normal physiology as well as the pathology of all multicellular organisms. These interactions have been shown to influence the growth, development, and maintenance of normal tissue function. In previous studies, we have shown that neonatal cardiac myocytes specifically interacts with a variety of ECM components including fibronectin, laminin, and collagens I, III and IV. Culturing neonatal myocytes on laminin and collagen IV induces an increased rate of both cell spreading and sarcomerogenesis.

Organotypic arrangement of mouse embryonic lung cells on a basement membrane extract: involvement of laminin

Development ◽  
1990 ◽  
Vol 110 (4) ◽  
pp. 1091-1099 ◽  
Author(s):  
L. Schuger ◽  
K.S. O'Shea ◽  
B.B. Nelson ◽  
J. Varani
Keyword(s):  
Pattern Formation ◽  
Basement Membrane ◽  
Cell Aggregation ◽  
Cyst Formation ◽  
Cell Populations ◽  
Lung Cells ◽  
Type I ◽  
Embryonic Lung ◽  
Basement Membrane Extract ◽  
Membrane Extract

The behavior of embryonic murine lung cells on a basement membrane extract (Matrigel) was investigated. Single cell suspensions generated by trypsinization of lungs removed from day 12 embryos were plated on Matrigel and cultured for up to one week. The basement membrane extract was used as a gel, and as a wet or dried film. In all of these instances, organotypic arrangement of the embryonic lung cells was observed. This process consisted of cell aggregation, sorting, polarization and formation of a tridimensional organization resembling embryonic lung. The maximal degree of organotypic development was obtained by using a thick gel; minimal reorganization was observed using a dried film. A rabbit polyclonal serum to laminin inhibited organotypic pattern formation while normal rabbit serum did not. Culture of lung cells on laminin gels promoted epithelial cyst formation but poor mesenchymal organization. By studying the behavior of epithelial and/or mesenchymal enriched cell populations on Matrigel, it was concluded that organotypic pattern formation on Matrigel required the presence of both cell populations. Cultivation of dissociated lung cells on a gel consisting of a mixture of collagens type I and III (Vitrogen-100) produced only cell aggregation. Cultivation of lung cells on a thin film of Vitrogen-100 or on uncoated tissue culture plastic produced monolayers of mesenchymal cells alone. Cultivation of lung cells in suspension also failed to induce organotypic arrangement even at maximal cell densities. The present study strongly supports a role for the basement membrane in the organotypic rearrangement of embryonic lung cells and subsequent in vitro cyst formation and budding of the reestablished epithelium. This, in turn, reinforces the concept of the basement membrane as a major regulator of organogenesis.

scholarly journals In situ luminescence analysis: a new light on monitoring calcium phosphate phase transitions

2017 ◽  
Vol 4 (7) ◽  
pp. 1157-1165 ◽  
Author(s):  
H. Terraschke ◽  
M. Rothe ◽  
A.-M. Tsirigoni ◽  
P. Lindenberg ◽  
L. Ruiz Arana ◽  
...  
Keyword(s):  
Phase Transitions ◽  
Calcium Phosphate ◽  
Synchrotron Radiation ◽  
Calcium Phosphates ◽  
High Sensitivity ◽  
Luminescence Analysis ◽  
Biologically Relevant ◽  
Phosphate Phase ◽  
Calcium Phosphate Phase

In situ luminescence measurements allow monitoring the phase transitions of biologically relevant calcium phosphates with high sensitivity, independent of synchrotron radiation.

Differentiation of Primary Human Submandibular Gland Cells Cultured on Basement Membrane Extract

2008 ◽  
Vol 14 (11) ◽  
pp. 1915-1926 ◽  
Author(s):  
Vanda Szlávik ◽  
Bálint Szabó ◽  
Tamás Vicsek ◽  
József Barabás ◽  
Sándor Bogdán ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Submandibular Gland ◽  
Gland Cells ◽  
Basement Membrane Extract ◽  
Membrane Extract ◽  
Cells Cultured

scholarly journals Basement membrane extract attenuates the more malignant gene expression profile accentuated by fibronectin in prostate cancer cells

2018 ◽  
Vol 451 (1-2) ◽  
pp. 131-138 ◽  
Author(s):  
Bruno Martinucci ◽  
Brenda de Carvalho Minatel ◽  
Maira Smaniotto Cucielo ◽  
Mariana Medeiros ◽  
Ivan José Vechetti-Junior ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Basement Membrane ◽  
Cancer Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Prostate Cancer Cells ◽  
Basement Membrane Extract ◽  
Membrane Extract

scholarly journals Responsiveness to basement membrane extract as a possible trait for tumorigenicity characterization

Vaccine X ◽  
2019 ◽  
Vol 1 ◽  
pp. 100004
Author(s):  
Haruhiko Murata ◽  
Romelda Omeir ◽  
Wei Tu ◽  
Lynda Lanning ◽  
Kathryn Phy ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Basement Membrane Extract ◽  
Membrane Extract
Sign in / Sign up

Export Citation Format

Share Document