scholarly journals Supramolecular fluorogenic peptide sensor array based on graphene oxide for the differential sensing of ebola virus

2020 ◽  
Vol 56 (43) ◽  
pp. 5735-5738 ◽  
Author(s):  
Meng-Qi Fu ◽  
Xu-Chen Wang ◽  
Wei-Tao Dou ◽  
Guo-Rong Chen ◽  
Tony D. James ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Graphene Oxide ◽  
Vesicular Stomatitis Virus ◽  
Sensor Array ◽  
Ebola Virus ◽  
Principal Component ◽  
Component Analysis ◽  
Marburg Virus ◽  
Vesicular Stomatitis ◽  
Fluorogenic Peptide

Principal component analysis of a fluorescent supramolecular sensor array based on graphene oxide can be used to differentiate ebola virus from marburg virus and receptor-extensive vesicular stomatitis virus.

scholarly journals Lassa-Vesicular Stomatitis Chimeric Virus Safely Destroys Brain Tumors

2015 ◽  
Vol 89 (13) ◽  
pp. 6711-6724 ◽  
Author(s):  
Guido Wollmann ◽  
Eugene Drokhlyansky ◽  
John N. Davis ◽  
Connie Cepko ◽  
Anthony N. van den Pol
Keyword(s):  
Brain Tumors ◽  
Vesicular Stomatitis Virus ◽  
Brain Cancer ◽  
Ebola Virus ◽  
Marburg Virus ◽  
Chimeric Virus ◽  
Lassa Virus ◽  
Gene Coding ◽  
Vesicular Stomatitis ◽  
The Brain

ABSTRACTHigh-grade tumors in the brain are among the deadliest of cancers. Here, we took a promising oncolytic virus, vesicular stomatitis virus (VSV), and tested the hypothesis that the neurotoxicity associated with the virus could be eliminated without blocking its oncolytic potential in the brain by replacing the neurotropic VSV glycoprotein with the glycoprotein from one of five different viruses, including Ebola virus, Marburg virus, lymphocytic choriomeningitis virus (LCMV), rabies virus, and Lassa virus. Based onin vitroinfections of normal and tumor cells, we selected two viruses to testin vivo. Wild-type VSV was lethal when injected directly into the brain. In contrast, a novel chimeric virus (VSV-LASV-GPC) containing genes from both the Lassa virus glycoprotein precursor (GPC) and VSV showed no adverse actions within or outside the brain and targeted and completely destroyed brain cancer, including high-grade glioblastoma and melanoma, even in metastatic cancer models. When mice had two brain tumors, intratumoral VSV-LASV-GPC injection in one tumor (glioma or melanoma) led to complete tumor destruction; importantly, the virus moved contralaterally within the brain to selectively infect the second noninjected tumor. A chimeric virus combining VSV genes with the gene coding for the Ebola virus glycoprotein was safe in the brain and also selectively targeted brain tumors but was substantially less effective in destroying brain tumors and prolonging survival of tumor-bearing mice. A tropism for multiple cancer types combined with an exquisite tumor specificity opens a new door to widespread application of VSV-LASV-GPC as a safe and efficacious oncolytic chimeric virus within the brain.IMPORTANCEMany viruses have been tested for their ability to target and kill cancer cells. Vesicular stomatitis virus (VSV) has shown substantial promise, but a key problem is that if it enters the brain, it can generate adverse neurologic consequences, including death. We tested a series of chimeric viruses containing genes coding for VSV, together with a gene coding for the glycoprotein from other viruses, including Ebola virus, Lassa virus, LCMV, rabies virus, and Marburg virus, which was substituted for the VSV glycoprotein gene. Ebola and Lassa chimeric viruses were safe in the brain and targeted brain tumors. Lassa-VSV was particularly effective, showed no adverse side effects even when injected directly into the brain, and targeted and destroyed two different types of deadly brain cancer, including glioblastoma and melanoma.

scholarly journals Recombinant Vesicular Stomatitis Virus Vector Mediates Postexposure Protection against Sudan Ebola Hemorrhagic Fever in Nonhuman Primates

2008 ◽  
Vol 82 (11) ◽  
pp. 5664-5668 ◽  
Author(s):  
Thomas W. Geisbert ◽  
Kathleen M. Daddario-DiCaprio ◽  
Kinola J. N. Williams ◽  
Joan B. Geisbert ◽  
Anders Leung ◽  
...  
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Rhesus Monkeys ◽  
Nonhuman Primates ◽  
Ebola Virus ◽  
Rhesus Macaques ◽  
Hemorrhagic Fever ◽  
Marburg Virus ◽  
Ebola Hemorrhagic Fever ◽  
Zaire Ebolavirus ◽  
Vesicular Stomatitis

ABSTRACT Recombinant vesicular stomatitis virus (VSV) vectors expressing homologous filoviral glycoproteins can completely protect rhesus monkeys against Marburg virus when administered after exposure and can partially protect macaques after challenge with Zaire ebolavirus. Here, we administered a VSV vector expressing the Sudan ebolavirus (SEBOV) glycoprotein to four rhesus macaques shortly after exposure to SEBOV. All four animals survived SEBOV challenge, while a control animal that received a nonspecific vector developed fulminant SEBOV hemorrhagic fever and succumbed. This is the first demonstration of complete postexposure protection against an Ebola virus in nonhuman primates and provides further evidence that postexposure vaccination may have utility in treating exposures to filoviruses.

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