A fluorescent naphthalimide NADH mimic for continuous and reversible sensing of cellular redox state

2020 ◽  
Vol 56 (15) ◽  
pp. 2240-2243 ◽  
Author(s):  
Hemant Sharma ◽  
Nian Kee Tan ◽  
Natalie Trinh ◽  
Jia Hao Yeo ◽  
Elizabeth J. New ◽  
...  
Keyword(s):  
Redox State ◽  
Cellular Redox ◽  
Cellular Redox State

A new naphthalimide based NADH mimic that functions as a fully reversible fluorescent “on off” probe for redox state has been synthesised and evaluated.

scholarly journals Cellular Redox State Acts as Switch to Determine the Direction of NNT-Catalyzed Reaction in Cystic Fibrosis Cells

2021 ◽  
Vol 22 (2) ◽  
pp. 967
Author(s):  
Maria Favia ◽  
Anna Atlante
Keyword(s):  
Cystic Fibrosis ◽  
Redox State ◽  
Molecular Mechanisms ◽  
Activity Level ◽  
Cellular Redox ◽  
Redox States ◽  
Mitochondrial Ros ◽  
New Information ◽  
Cellular Dysfunction ◽  
Cellular Redox State

The redox states of NAD and NADP are linked to each other in the mitochondria thanks to the enzyme nicotinamide nucleotide transhydrogenase (NNT) which, by utilizing the mitochondrial membrane potential (mΔΨ), catalyzes the transfer of redox potential between these two coenzymes, reducing one at the expense of the oxidation of the other. In order to define NNT reaction direction in CF cells, NNT activity under different redox states of cell has been investigated. Using spectrophotometric and western blotting techniques, the presence, abundance and activity level of NNT were determined. In parallel, the levels of NADPH and NADH as well as of mitochondrial and cellular ROS were also quantified. CF cells showed a 70% increase in protein expression compared to the Wt sample; however, regarding NNT activity, it was surprisingly lower in CF cells than healthy cells (about 30%). The cellular redox state, together with the low mΔΨ, pushes to drive NNT reverse reaction, at the expense of its antioxidant potential, thus consuming NADPH to support NADH production. At the same time, the reduced NNT activity prevents the NADH, produced by the reaction, from causing an explosion of ROS by the damaged respiratory chain, in accordance with the reduced level of mitochondrial ROS in NNT-loss cells. This new information on cellular bioenergetics represents an important building block for further understanding the molecular mechanisms responsible for cellular dysfunction in cystic fibrosis.

A Novel Redox Based Therapy Targets the Malignant Cellular Redox State

2016 ◽  
Vol 100 ◽  
pp. S119 ◽  
Author(s):  
Dustin Carroll ◽  
Yanming Zhao ◽  
Haining Zhu ◽  
Ines Batinic-Haberle ◽  
Daret St. Clair
Keyword(s):  
Redox State ◽  
Cellular Redox ◽  
Therapy Targets ◽  
Cellular Redox State

Mitochondria and cellular redox state on the route from ageing to Alzheimer’s disease

2020 ◽  
Vol 192 ◽  
pp. 111385 ◽  
Author(s):  
G. Abate ◽  
M. Vezzoli ◽  
M. Sandri ◽  
W. Rungratanawanich ◽  
M. Memo ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Redox State ◽  
Cellular Redox ◽  
Cellular Redox State

scholarly journals Phosphorylation of a p38-like MAPK is involved in sensing cellular redox state and drives atypical tubulin polymer assembly in angiosperms

2013 ◽  
Vol 37 (5) ◽  
pp. 1130-1143 ◽  
Author(s):  
PANTELIS LIVANOS ◽  
BASIL GALATIS ◽  
CATHERINE GAITANAKI ◽  
PANAGIOTIS APOSTOLAKOS
Keyword(s):  
Redox State ◽  
Cellular Redox ◽  
Cellular Redox State ◽  
Polymer Assembly

scholarly journals Modulation of Pseudomonas aeruginosa Quorum Sensing by Glutathione

2019 ◽  
Vol 201 (9) ◽  
Author(s):  
Hui Zhou ◽  
Meizhen Wang ◽  
Nicole E. Smalley ◽  
Maxim Kostylev ◽  
Amy L. Schaefer ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Quorum Sensing ◽  
Cell Density ◽  
Redox State ◽  
Gene Activation ◽  
Cellular Redox ◽  
Content Type ◽  
Cellular Redox State

ABSTRACT Pseudomonas aeruginosa uses quorum sensing (QS) to regulate the production of a battery of secreted products. At least some of these products are shared among the population and serve as public goods. When P. aeruginosa is grown on casein as the sole carbon and energy source, the QS-induced extracellular protease elastase is required for growth. We isolated a P. aeruginosa variant, which showed increased production of QS-induced factors after repeated transfers in casein broth. This variant, P. aeruginosa QS*, had a mutation in the glutathione synthesis gene gshA. We describe several experiments that show a gshA coding variant and glutathione affect the QS response. The P. aeruginosa QS transcription factor LasR has a redox-sensitive cysteine (C79). We report that GshA variant cells with a LasR C79S substitution show a similar QS response to that of wild-type P. aeruginosa. Surprisingly, it is not LasR but the QS transcription factor RhlR that is more active in bacteria containing the variant gshA. Our results demonstrate that QS integrates information about cell density and the cellular redox state via glutathione levels. IMPORTANCE Pseudomonas aeruginosa and other bacteria coordinate group behaviors using a chemical communication system called quorum sensing (QS). The QS system of P. aeruginosa is complex, with several regulators and signals. We show that decreased levels of glutathione lead to increased gene activation in P. aeruginosa, which did not occur in a strain carrying the redox-insensitive variant of a transcription factor. The ability of P. aeruginosa QS transcription factors to integrate information about cell density and cellular redox state shows these transcription factors can fine-tune levels of the gene products they control in response to at least two types of signals or cues.

scholarly journals Cellular redox state constrains serine synthesis and nucleotide production to impact cell proliferation

2019 ◽  
Vol 1 (9) ◽  
pp. 861-867 ◽  
Author(s):  
Frances F. Diehl ◽  
Caroline A. Lewis ◽  
Brian P. Fiske ◽  
Matthew G. Vander Heiden
Keyword(s):  
Cell Proliferation ◽  
Redox State ◽  
Cellular Redox ◽  
Cellular Redox State
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