Cyclization of a G4-specific peptide enhances its stability and G-quadruplex binding affinity

2020 ◽  
Vol 56 (7) ◽  
pp. 1082-1084 ◽  
Author(s):  
Khac Huy Ngo ◽  
Renliang Yang ◽  
Poulomi Das ◽  
Giang K. T. Nguyen ◽  
Kah Wai Lim ◽  
...  
Keyword(s):  
Binding Affinity ◽  
G Quadruplex ◽  
Specific Peptide ◽  
Head To Tail Cyclization

Head-to-tail cyclization of a G-quadruplex-specific peptide was shown to enhance its stability and G-quadruplex binding affinity.

Studies on the Material Properties of Platinum Complex Binding to Human Telomeric G-Quadruplex DNA

2013 ◽  
Vol 700 ◽  
pp. 63-66
Author(s):  
Xu Jian Luo ◽  
Qi Pin Qin ◽  
Yu Lan Li ◽  
Yan Cheng Liu
Keyword(s):  
Binding Affinity ◽  
Material Properties ◽  
Platinum Complex ◽  
Spectroscopic Methods ◽  
Binding Properties ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
New Material ◽  
Binding Intensity ◽  
Ct Dna

A new material of cation platinum (II) complex has been synthesized and characterized. The new material binding properties with human telomeric G-quadruplex DNA (G4-Htel DNA) and ct-DNA were examined by UV-Vis and CD spectroscopic methods. The results showed that complex exhibited higher binding affinity and binding intensity to G4-Htel DNA (up to Kb = 1.54×106 M-1) and with selectivity (up to 11-fold) over duplex DNA. The CD results suggests the antiparallel structure of G-quadruplex can remain stable in the presence of platinum complex and the complex may bind to DNA by intercalation mode.

scholarly journals Stapling a G-quadruplex specific peptide

2020 ◽  
Vol 531 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Militsa Yavorova Yaneva ◽  
Vee Vee Cheong ◽  
Jun Kee Cheng ◽  
Kah Wai Lim ◽  
Anh Tuân Phan
Keyword(s):  
G Quadruplex ◽  
Specific Peptide

Aptamer-induced thermofluorimetric protein stabilization and G-quadruplex nucleic acid staining by SYPRO orange dye

2019 ◽  
Vol 43 (13) ◽  
pp. 4994-4997 ◽  
Author(s):  
Prashant S. Deore ◽  
Richard A. Manderville
Keyword(s):  
Nucleic Acid ◽  
Dissociation Constant ◽  
Melting Temperature ◽  
Binding Affinity ◽  
Thermal Stabilization ◽  
Protein Stabilization ◽  
Dna Aptamer ◽  
G Quadruplex

Serendipitously discovered nucleic acid staining by SYPRO Orange dye utilized to demonstrate proteins thermal stabilization (increase in melting temperature,Tm) as a function of increased DNA aptamer binding affinity (decrease in dissociation constant,Kd).

scholarly journals Dimeric and Multimeric DNA Aptamers for Highly Effective Protein Recognition

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5227 ◽  
Author(s):  
Claudia Riccardi ◽  
Ettore Napolitano ◽  
Domenica Musumeci ◽  
Daniela Montesarchio
Keyword(s):  
Binding Affinity ◽  
Anticancer Agents ◽  
Building Blocks ◽  
Protein Recognition ◽  
Therapeutic Activity ◽  
G Quadruplex ◽  
Biological Target ◽  
Activity Enhancement ◽  
Improved Performance ◽  
Target Binding

Multivalent interactions frequently occur in biological systems and typically provide higher binding affinity and selectivity in target recognition than when only monovalent interactions are operative. Thus, taking inspiration by nature, bivalent or multivalent nucleic acid aptamers recognizing a specific biological target have been extensively studied in the last decades. Indeed, oligonucleotide-based aptamers are suitable building blocks for the development of highly efficient multivalent systems since they can be easily modified and assembled exploiting proper connecting linkers of different nature. Thus, substantial research efforts have been put in the construction of dimeric/multimeric versions of effective aptamers with various degrees of success in target binding affinity or therapeutic activity enhancement. The present review summarizes recent advances in the design and development of dimeric and multimeric DNA-based aptamers, including those forming G-quadruplex (G4) structures, recognizing different key proteins in relevant pathological processes. Most of the designed constructs have shown improved performance in terms of binding affinity or therapeutic activity as anti-inflammatory, antiviral, anticoagulant, and anticancer agents and their number is certainly bound to grow in the next future.

A Metal-Mediated Conformational Switch Controls G-Quadruplex Binding Affinity

2008 ◽  
Vol 47 (26) ◽  
pp. 4858-4861 ◽  
Author(s):  
David Monchaud ◽  
Peng Yang ◽  
Laurent Lacroix ◽  
Marie-Paule Teulade-Fichou ◽  
Jean-Louis Mergny
Keyword(s):  
Binding Affinity ◽  
Conformational Switch ◽  
G Quadruplex

Spectroscopic study and G-quadruplex DNA binding affinity of two bioactive papaverine-derived ligands

2007 ◽  
Vol 41 (5) ◽  
pp. 558-563 ◽  
Author(s):  
Elzbieta Galezowska ◽  
Anna Masternak ◽  
Blazej Rubis ◽  
Artur Czyrski ◽  
Maria Rybczyńska ◽  
...  
Keyword(s):  
Dna Binding ◽  
Spectroscopic Study ◽  
Binding Affinity ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Dna Binding Affinity
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