A pyrophosphate-activated nanochannel inspired by a TRP ion channel

2019 ◽  
Vol 55 (85) ◽  
pp. 12833-12836 ◽  
Author(s):  
Fei Zhu ◽  
Guanxing Yang ◽  
Manivannan Kalavathi Dhinakaran ◽  
Rui Wang ◽  
Miaomiao Song ◽  
...  
Keyword(s):  
Ion Channel ◽  
Trp Ion Channel

An artificial pyrophosphate (PPi) selectively activated nanochannel has been successfully constructed via introducing calix[4]arene receptors into a biomimetic nanochannel.

A cell-based impedance assay for monitoring transient receptor potential (TRP) ion channel activity

2011 ◽  
Vol 26 (5) ◽  
pp. 2376-2382 ◽  
Author(s):  
Oliver Pänke ◽  
Winnie Weigel ◽  
Sabine Schmidt ◽  
Anja Steude ◽  
Andrea A. Robitzki
Keyword(s):  
Ion Channel ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Channel Activity ◽  
A Cell ◽  
Transient Receptor ◽  
Trp Ion Channel

scholarly journals Single nucleotide polymorphisms and genotypes of transient receptor potential ion channel and acetylcholine receptor genes from isolated B lymphocytes in myalgic encephalomyelitis/chronic fatigue syndrome patients

2016 ◽  
Vol 44 (6) ◽  
pp. 1381-1394 ◽  
Author(s):  
Sonya Marshall-Gradisnik ◽  
Samantha Johnston ◽  
Anu Chacko ◽  
Thao Nguyen ◽  
Peter Smith ◽  
...  
Keyword(s):  
B Cells ◽  
Chronic Fatigue Syndrome ◽  
Single Nucleotide Polymorphisms ◽  
Ion Channel ◽  
B Cell ◽  
Chronic Fatigue ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Fatigue Syndrome ◽  
Trp Ion Channel

Objective The pathomechanism of chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is unknown; however, a small subgroup of patients has shown muscarinic antibody positivity and reduced symptom presentation following anti-CD20 intervention. Given the important roles of calcium (Ca2+) and acetylcholine (ACh) signalling in B cell activation and potential antibody development, we aimed to identify relevant single nucleotide polymorphisms (SNPs) and genotypes in isolated B cells from CFS/ME patients. Methods A total of 11 CFS/ME patients (aged 31.82 ± 5.50 years) and 11 non-fatigued controls (aged 33.91 ± 5.06 years) were included. Flow cytometric protocols were used to determine B cell purity, followed by SNP and genotype analysis for 21 mammalian TRP ion channel genes and nine mammalian ACh receptor genes. SNP association and genotyping analysis were performed using ANOVA and PLINK analysis software. Results Seventy-eight SNPs were identified in nicotinic and muscarinic acetylcholine receptor genes in the CFS/ME group, of which 35 were in mAChM3. The remaining SNPs were identified in nAChR delta (n = 12), nAChR alpha 9 (n = 5), TRPV2 (n = 7), TRPM3 (n = 4), TRPM4 (n = 1) mAChRM3 2 (n = 2), and mAChRM5 (n = 3) genes. Nine genotypes were identified from SNPs in TRPM3 (n = 1), TRPC6 (n = 1), mAChRM3 (n = 2), nAChR alpha 4 (n = 1), and nAChR beta 1 (n = 4) genes, and were located in introns and 3′ untranslated regions. Odds ratios for these specific genotypes ranged between 7.11 and 26.67 for CFS/ME compared with the non-fatigued control group. Conclusion This preliminary investigation identified a number of SNPs and genotypes in genes encoding TRP ion channels and AChRs from B cells in patients with CFS/ME. These may be involved in B cell functional changes, and suggest a role for Ca2+ dysregulation in AChR and TRP ion channel signalling in the pathomechanism of CFS/ME.

scholarly journals Alterations in the Ca2+ toolkit in oesophageal adenocarcinoma

2021 ◽  
Vol 2 (6) ◽  
Author(s):  
Alana L. Cutliffe ◽  
Sharon L. McKenna ◽  
Darshan S. Chandrashekar ◽  
Alvin Ng ◽  
Ginny Devonshire ◽  
...  
Keyword(s):  
Ion Channel ◽  
Oesophageal Cancer ◽  
Normal Tissue ◽  
Patient Survival ◽  
Oesophageal Adenocarcinoma ◽  
Survival Outcomes ◽  
Altered Expression ◽  
Voltage Gated ◽  
Molecular Stratification ◽  
Trp Ion Channel

Aim: To investigate alterations in transcription of genes, encoding Ca2+ toolkit proteins, in oesophageal adenocarcinoma (OAC) and to assess associations between gene expression, tumor grade, nodal-metastatic stage, and patient survival. Methods: The expression of 275 transcripts, encoding components of the Ca2+ toolkit, was analyzed in two OAC datasets: the Cancer Genome Atlas [via the University of Alabama Cancer (UALCAN) portal] and the oesophageal-cancer, clinical, and molecular stratification [Oesophageal Cancer Clinical and Molecular Stratification (OCCAMS)] dataset. Effects of differential expression of these genes on patient survival were determined using Kaplan-Meier log-rank tests. OAC grade- and metastatic-stage status was investigated for a subset of genes. Adjustment for the multiplicity of testing was made throughout. Results: Of the 275 Ca2+-toolkit genes analyzed, 75 displayed consistent changes in expression between OAC and normal tissue in both datasets. The channel-encoding genes, N-methyl-D-aspartate receptor 2D (GRIN2D), transient receptor potential (TRP) ion channel classical or canonical 4 (TRPC4), and TRP ion channel melastatin 2 (TRPM2) demonstrated the greatest increase in expression in OAC in both datasets. Nine genes were consistently upregulated in both datasets and were also associated with improved survival outcomes. The 6 top-ranking genes for the weighted significance of altered expression and survival outcomes were selected for further analysis: voltage-gated Ca2+ channel subunit α 1D (CACNA1D), voltage-gated Ca2+ channel auxiliary subunit α2 δ4 (CACNA2D4), junctophilin 1 (JPH1), acid-sensing ion channel 4 (ACCN4), TRPM5, and secretory pathway Ca2+ ATPase 2 (ATP2C2). CACNA1D, JPH1, and ATP2C2 were also upregulated in advanced OAC tumor grades and nodal-metastatic stages in both datasets. Conclusions: This study has unveiled alterations of the Ca2+ toolkit in OAC, compared to normal tissue. Such Ca2+ signalling findings are consistent with those from studies on other cancers. Genes that were consistently upregulated in both datasets might represent useful markers for patient diagnosis. Genes that were consistently upregulated, and which were associated with improved survival, might be useful markers for patient outcome. These survival-associated genes may also represent targets for the development of novel chemotherapeutic agents.

The capsaicin receptor

2018 ◽  
Author(s):  
Istvan Nagy
Keyword(s):  
Ion Channel ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Chilli Pepper ◽  
Capsaicin Receptor ◽  
Major Cation ◽  
Starting Point ◽  
Pain Pathway ◽  
Transient Receptor ◽  
Trp Ion Channel

The landmark paper discussed in this chapter is ‘The capsaicin receptor: A heat activated ion channel in the pain pathway’, published by Caterina et al. in 1997. The identification of the molecular basis for the sensitivity of a major proportion of nociceptive primary sensory neurons for capsaicin, the pungent agent in chilli pepper, was undoubtedly one of the most significant pain-related discoveries in the twentieth century, for at least three reasons. First, the mechanism for capsaicin-induced responses could unequivocally be explained. Second, the discovery heralded the starting point for the development of a highly promising, mechanism-based means of analgesia. Third, the discovery also sparked studies which resulted in the discovery of the major cation channel family, the transient receptor potential (TRP) ion channel family, several members of which have also become putative targets for the development of analgesics.

Behavioral role of the canonical transient receptor potential (TRP) ion channel, TRP‐1, in C. elegans

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Ana Y. Estevez ◽  
Brian Aucter ◽  
Charles Dirac ◽  
Thomas Lepesqueur ◽  
Alejandra Marrero ◽  
...  
Keyword(s):  
Ion Channel ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
C Elegans ◽  
Canonical Transient Receptor Potential ◽  
Transient Receptor ◽  
Trp Ion Channel
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