scholarly journals Multivalent glycoligands with lectin/enzyme dual specificity: self-deliverable glycosidase regulators

2019 ◽  
Vol 55 (85) ◽  
pp. 12845-12848 ◽  
Author(s):  
Manuel González-Cuesta ◽  
David Goyard ◽  
Eiji Nanba ◽  
Katsumi Higaki ◽  
José M. García Fernández ◽  
...  
Keyword(s):  
Mannose Receptor ◽  
Dual Specificity ◽  
Macrophage Mannose Receptor ◽  
Lysosomal Glycosidases

Multimannosides assembled onto cyclodextrin or cyclopeptide cores exhibit dual specificity towards the macrophage mannose receptor and lysosomal glycosidases.

HIV Impairs Alveolar Macrophage Mannose Receptor Function Against Pneumocystis carinii

CHEST Journal ◽  
1993 ◽  
Vol 103 (2) ◽  
pp. 111S-112S ◽  
Author(s):  
Henry Koziel ◽  
B.A. Kruskal ◽  
R.A.B Ezekowitz ◽  
R.M Rose
Keyword(s):  
Alveolar Macrophage ◽  
Pneumocystis Carinii ◽  
Mannose Receptor ◽  
Receptor Function ◽  
Macrophage Mannose Receptor

Dexamethasone Blocks the Interferon-γ-Mediated Downregulation of the Macrophage Mannose Receptor

1994 ◽  
Vol 312 (2) ◽  
pp. 367-374 ◽  
Author(s):  
V.L. Shepherd ◽  
H.B. Cowan ◽  
R. Abdolrasulnia ◽  
S. Vick
Keyword(s):  
Mannose Receptor ◽  
Interferon Γ ◽  
Macrophage Mannose Receptor

scholarly journals Absence of the Macrophage Mannose Receptor in Mice Does Not Increase Susceptibility to Pneumocystis carinii Infection In Vivo

2003 ◽  
Vol 71 (11) ◽  
pp. 6213-6221 ◽  
Author(s):  
Steve D. Swain ◽  
Sena J. Lee ◽  
Michel C. Nussenzweig ◽  
Allen G. Harmsen
Keyword(s):  
Pneumocystis Carinii ◽  
Hydrolytic Enzymes ◽  
Mannose Receptor ◽  
Opportunistic Pathogen ◽  
Cell Types ◽  
Surface Expression ◽  
Wild Type ◽  
Macrophage Mannose Receptor

ABSTRACT Host defense against the opportunistic pathogen Pneumocystis carinii requires functional interactions of many cell types. Alveolar macrophages are presumed to be a vital host cell in the clearance of P. carinii, and the mechanisms of this interaction have come under scrutiny. The macrophage mannose receptor is believed to play an important role as a receptor involved in the binding and phagocytosis of P. carinii. Although there is in vitro evidence for this interaction, the in vivo role of this receptor in P. carinii clearance in unclear. Using a mouse model in which the mannose receptor has been deleted, we found that the absence of this receptor is not sufficient to allow infection by P. carinii in otherwise immunocompetent mice. Furthermore, when mice were rendered susceptible to P. carinii by CD4+ depletion, mannose receptor knockout mice (MR-KO) had pathogen loads equal to those of wild-type mice. However, the MR-KO mice exhibited a greater influx of phagocytes into the alveoli during infection. This was accompanied by increased pulmonary pathology in the MR-KO mice, as well as greater accumulation of glycoproteins in the alveoli (glycoproteins, including harmful hydrolytic enzymes, are normally cleared by the mannose receptor). We also found that the surface expression of the mannose receptor is not downregulated during P. carinii infection in wild-type mice. Our findings suggest that while the macrophage mannose receptor may be important in the recognition of P. carinii, in vivo, this mechanism may be redundant, and the absence of this receptor may be compensated for.

scholarly journals Mannosylated Polyrotaxanes for Increasing Cellular Uptake Efficiency in Macrophages through Receptor-Mediated Endocytosis

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 439 ◽  
Author(s):  
Kai Shibaguchi ◽  
Atsushi Tamura ◽  
Masahiko Terauchi ◽  
Mitsuaki Matsumura ◽  
Hiroyuki Miura ◽  
...  
Keyword(s):  
Targeted Delivery ◽  
Inflammatory Diseases ◽  
Specific Interaction ◽  
Mannose Receptor ◽  
Raw264.7 Cells ◽  
Intracellular Uptake ◽  
Macrophage Mannose Receptor ◽  
Selective Targeting ◽  
Poly Ethylene ◽  
Nih 3T3

Macrophages play an important role in the regulation of inflammation and immune response as well as the pathogenesis of chronic inflammatory diseases and cancer. Therefore, targeted delivery of therapeutic reagents to macrophages is an effective method for treatment and diagnosis. We previously examined the therapeutic applications of polyrotaxanes (PRXs) comprised of multiple cyclodextrins (CDs) threaded on a polymer chain and capped with bulky stopper molecules. In the present study, we designed an α-d-mannose-modified α-CD/poly(ethylene glycol)-based PRX (Man-PRX). The intracellular uptake of Man-PRX through the interaction with macrophage mannose receptor (MMR) in macrophage-like RAW264.7 cells was examined. Intracellular Man-PRX uptake was observed in MMR-positive RAW264.7 cells but was negligible in MMR-negative NIH/3T3 cells. In addition, the intracellular Man-PRX uptake in RAW264.7 cells was significantly inhibited in the presence of free α-d-mannose and an anti-MMR antibody, which suggests that MMR is involved in the intracellular uptake of Man-PRX. Moreover, the polarization of RAW264.7 cells affected the Man-PRX internalization efficiency. These results indicate that Man-PRX is an effective candidate for selective targeting of macrophages through a specific interaction with the MMR.

Sign in / Sign up

Export Citation Format

Share Document