A PANI supported lipid bilayer that contains NhaA transporter proteins provides a basis for a biomimetic biocapacitor

2019 ◽  
Vol 55 (87) ◽  
pp. 13152-13155
Author(s):  
Awatef Ben Tahar ◽  
Abdelkader Zebda ◽  
Jean-Pierre Alcaraz ◽  
Landry Gayet ◽  
Abderrahim Boualam ◽  
...  
Keyword(s):  
Ion Transport ◽  
Lipid Bilayer ◽  
High Speed ◽  
Membrane System ◽  
Transport Proteins ◽  
Biological Engineering ◽  
Supported Lipid Bilayer ◽  
Transporter Proteins ◽  
Electrogenic Ion Transport ◽  
Biomimetic Membrane

This biomimetic membrane system of Na+/H+ transport proteins in a lipid bilayer supported by polyanaline has controllable electrogenic ion transport to function as a high-speed rechargeable biocapacitor for use in bioinspired biological engineering.

Potassium Ion Transport by Valinomycin across a Hg-Supported Lipid Bilayer

2005 ◽  
Vol 127 (38) ◽  
pp. 13316-13323 ◽  
Author(s):  
Lucia Becucci ◽  
Maria Rosa Moncelli ◽  
Renate Naumann ◽  
Rolando Guidelli
Keyword(s):  
Ion Transport ◽  
Lipid Bilayer ◽  
Potassium Ion ◽  
Supported Lipid Bilayer ◽  
Potassium Ion Transport

Elementary auxin response chains at the plasma membrane involve external abp1 and multiple electrogenic ion transport proteins

1996 ◽  
Vol 18 (1-2) ◽  
pp. 23-28 ◽  
Author(s):  
H�l�ne Barbier-Brygoo ◽  
Sabine Zimmermann ◽  
S�bastien Thomine ◽  
Ian R. White ◽  
Paul Millner ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Ion Transport ◽  
Transport Proteins ◽  
Auxin Response ◽  
Electrogenic Ion Transport ◽  
Response Chains

Biomimetic Membrane System Composed of a Composite Interpenetrating Hydrogel Film and a Lipid Bilayer

2012 ◽  
Vol 22 (20) ◽  
pp. 4259-4267 ◽  
Author(s):  
Barry Stidder ◽  
Jean-Pierre Alcaraz ◽  
Lavinia Liguori ◽  
Nawel Khalef ◽  
Aziz Bakri ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Membrane System ◽  
Hydrogel Film ◽  
Biomimetic Membrane

scholarly journals Stripping Material from a Supported Lipid Bilayer with High Speed Buffer Flow

2020 ◽  
Vol 17 (3) ◽  
pp. 51-59
Author(s):  
Michael Ornstead ◽  
Ruth Hunter ◽  
Mason Valentine ◽  
Cameron Cooper ◽  
Stephen Smith ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Microfluidic Device ◽  
High Speed ◽  
Membrane Vesicle ◽  
Microfluidic Channel ◽  
Lipid Vesicles ◽  
Membrane Properties ◽  
Supported Lipid Bilayer ◽  
Glass Coverslip

A microfluidic device was created and used to demonstrate that supported lipid bilayers can be deposited on clean glass slides and removed using high velocity buffer flow (1-4 m/s linear velocity). This was accomplished by forcing the flow through a microfluidic channel covering an annealed glass coverslip bearing a supported lipid bilayer (SLB). The removal of bilayer material was monitored via fluorescence microscopy, and two basic regimes were observed: at 1-2 m/s smaller areas were stripped, while at 3-4 m/s larger areas were stripped. SLB removal was verified by two means. First, lipid vesicles labeled with a different fluorescent dye were added to the device and filled in holes left by the removal of the original SLB, allowing stripping to be verified visually. Second, the solutions obtained from stripping were concentrated and the fluorescence in the concentrates was measured. The ability to strip SLB from glass provides a relatively gentle method of creating spatially inhomogeneous SLB, which could be a useful tool in the continued investigation of membrane properties and components. KEYWORDS: Supported Lipid Bilayer; Membrane Vesicle; Microfluidic Device

Membrane Alterations Induced by Amphiphiles

1989 ◽  
Vol 16 (3) ◽  
pp. 274-280
Author(s):  
Boris Isomaa ◽  
Henry Hägerstrand ◽  
Gun I.L. Paatero
Keyword(s):  
Ion Transport ◽  
Lipid Bilayer ◽  
Erythrocyte Membrane ◽  
Cell Shape ◽  
Osmotic Fragility ◽  
Molecular Shape ◽  
Specific Interactions ◽  
Amphiphilic Compounds ◽  
Rapid Formation ◽  
Polar Parts

Amphiphilic compounds with distinct apolar and polar parts are readily intercalated into the erythrocyte membrane. When intercalated into the membrane, amphiphiles are probably orientated so that the polar head is at the polar-apolar interface of the lipid bilayer and the hydrophobic part within the apolar core of the bilayer. However, by virtue of their difference in molecular shape from the bulk lipids of the lipid bilayer, it is possible that the intercalated amphiphiles are partly segregated from bulk lipids and accumulate at protein-lipid interfaces in the bilayer, where the packing of the bilayer lipids may be less ordered. Our studies show that amphiphiles, when intercalated into the erythrocyte membrane, trigger alterations in several membrane-connected functions. Some of the alterations induced (decreased osmotic fragility, increased passive potassium fluxes) seem to be due to non-specific interactions of the amphiphiles with the membrane, whereas other functions (ion transport mediated by membrane proteins, regulation of cell shape) seem to be sensitive to particular features of the amphiphiles. Our studies indicate that the intercalation of amphiphiles into the erythrocyte membrane must involve rearrangements within the lipid bilayer. We have suggested that, when intercalated into the lipid bilayer, amphiphiles trigger a rapid formation of non-bilayer phases, which protect the bilayer against a collapse and bring about a trans-bilayer redistribution of intercalated amphiphiles as well as of bilayer lipids. At high sublytic concentrations, this process may also involve a release of microvesicles from the membrane.

scholarly journals Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yutaro Shimizu ◽  
Junpei Takagi ◽  
Emi Ito ◽  
Yoko Ito ◽  
Kazuo Ebine ◽  
...  
Keyword(s):  
Membrane Trafficking ◽  
High Speed ◽  
Super Resolution ◽  
Adaptor Protein ◽  
Transport Proteins ◽  
3D Localization ◽  
Golgi Network ◽  
Distinct Distribution ◽  
Vacuolar Trafficking ◽  
Cargo Sorting

AbstractThe trans-Golgi network (TGN) has been known as a key platform to sort and transport proteins to their final destinations in post-Golgi membrane trafficking. However, how the TGN sorts proteins with different destinies still remains elusive. Here, we examined 3D localization and 4D dynamics of TGN-localized proteins of Arabidopsis thaliana that are involved in either secretory or vacuolar trafficking from the TGN, by a multicolor high-speed and high-resolution spinning-disk confocal microscopy approach that we developed. We demonstrate that TGN-localized proteins exhibit spatially and temporally distinct distribution. VAMP721 (R-SNARE), AP (adaptor protein complex)−1, and clathrin which are involved in secretory trafficking compose an exclusive subregion, whereas VAMP727 (R-SNARE) and AP-4 involved in vacuolar trafficking compose another subregion on the same TGN. Based on these findings, we propose that the single TGN has at least two subregions, or “zones”, responsible for distinct cargo sorting: the secretory-trafficking zone and the vacuolar-trafficking zone.

Characterization of Supported Lipid Bilayer Disruption By Chrysophsin-3 Using QCM-D

2011 ◽  
Vol 115 (51) ◽  
pp. 15228-15235 ◽  
Author(s):  
Kathleen F. Wang ◽  
Ramanathan Nagarajan ◽  
Charlene M. Mello ◽  
Terri A. Camesano
Keyword(s):  
Lipid Bilayer ◽  
Supported Lipid Bilayer
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