scholarly journals Heptylmannose-functionalized cellulose for the binding and specific detection of pathogenic E. coli

2019 ◽  
Vol 55 (68) ◽  
pp. 10158-10161 ◽  
Author(s):  
Madeleine Cauwel ◽  
Adeline Sivignon ◽  
Clarisse Bridot ◽  
Medy C. Nongbe ◽  
David Deniaud ◽  
...  
Keyword(s):  
Chemical Method ◽  
Cellulose Nanofibers ◽  
Specific Detection ◽  
E Coli ◽  
Cellulose Paper ◽  
Strong Affinity

We developed a chemical method to covalently functionalize cellulose nanofibers and cellulose paper with mannoside ligands displaying a strong affinity for the FimH adhesin from pathogenic E. coli strains.

Recent advances in optical biosensors for specific detection of E. coli bacteria in food and water

Food Control ◽  
2022 ◽  
pp. 108822
Author(s):  
Azam Bagheri Pebdeni ◽  
Amirreza Roshani ◽  
Ensiyeh Mirsadoughi ◽  
Shakila Behzadifar ◽  
Morteza Hosseini
Keyword(s):  
Optical Biosensors ◽  
Specific Detection ◽  
E Coli ◽  
Recent Advances

scholarly journals Studies on the Preservation of Raw Cow’s Milk by Chemical Method

1970 ◽  
Vol 42 (3) ◽  
pp. 317-326 ◽  
Author(s):  
F Rokhsana ◽  
UK Das ◽  
R Yeasmin ◽  
A Nahar ◽  
S Parveen
Keyword(s):  
Hydrogen Peroxide ◽  
Chemical Method ◽  
Room Temperature ◽  
Storage Period ◽  
Total Coliform ◽  
Human Consumption ◽  
Milk Products ◽  
E Coli ◽  
Keeping Quality ◽  
Control Milk

Studies carried out to develop a technique for the preservation of cow's milk in raw condition using hydrogen peroxide (H2O2) as a preservative. Fresh cow’s milk was collected and experiments were conducted by four treatments in order to achieve the optimum condition of storage. The treatments were with various concentration of H2O2 starting from 0.05 %, 0.1 %, 0.2 %, 0.3 %, 0.4 %, & 0.5 %. Treated milk with 0.05 % concentration of H2O2 had storage period of 20 days compared to that of the control one (5 days only) in refrigerated temperature (±8°C). On the other hand hydrogen peroxide treated milk (0.05 %) had a storage period of 8 hours at room temperature (±28°C). Results also showed that the higher concentration of H2O2 had no effect on storage period than that of control. Milk products like kheer and halawa prepared by treated milk and stored for 20 days showed almost nil growth of total coliform and E. coli which means that food products prepared from hydrogen peroxide treated milk is safe for human consumption. Key words: Raw, Storage, Hydrogen peroxide, Preservative, keeping quality, Pasteurization, deteriorated, MPN. Bangladesh J. Sci. Ind. Res. 42(3), 317-326, 2007

Biosensor for selective detection of E. coli in spinach using the strong affinity of derivatized mannose with fimbrial lectin

2014 ◽  
Vol 61 ◽  
pp. 266-273 ◽  
Author(s):  
Idris Yazgan ◽  
Naumih M. Noah ◽  
Ousmane Toure ◽  
Siyi Zhang ◽  
Omowunmi A. Sadik
Keyword(s):  
Selective Detection ◽  
E Coli ◽  
Strong Affinity

scholarly journals Elisa preliminary studies of immobilization and specific detection of bacterial strains

2020 ◽  
Vol 2 (2) ◽  
pp. 64-69
Author(s):  
Madalina Mihalache ◽  
◽  
Alina Banciu ◽  
Lucian Ionescu ◽  
Mihai Nita-Lazar
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescence Intensity ◽  
Polyclonal Antibody ◽  
Specific Binding ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Strains ◽  
Specific Detection ◽  
E Coli ◽  
Alexa Fluor

The paper aims to emphasize the specific detection of bacterial strains using enzyme-linked immunosorbent assay. The assay is based on the specific binding of polyclonal antibody anti-E. coli tagged with FITC to E.coli and monoclonal antibody anti-Ps. aeruginosa tagged with Alexa Fluor 647 tagged to Ps. aeruginosa and on subsequent enzymatic immunological demonstration of the conjugated enzyme. In this experiment, the negative control was the Salmonella enterica strain. The two antibodies had no interaction with the negative control, instead, they were specific for E. coli and Ps. aeruginosa strains. When both strains were in the same well, the fluorescence intensity given by the presence of E. coli was 2.3 times higher than that given by Ps. aeruginosa, and the intensity of fluorescence decreased if there are both bacterial strains in the wells.

Antibacterial Activity of the Silver Nanoparticles against Escherichia coli and Enterobacter sp

2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Kathirvel Maruthai ◽  
Kommoju Vallayyachari ◽  
Thirumurugan Ravibalan ◽  
Sheryl Ann Philip ◽  
Antony V. Samrot ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Chemical Method ◽  
Reduction Method ◽  
Clinical Importance ◽  
Bactericidal Effect ◽  
Bacterial Cells ◽  
E Coli ◽  
Protein Leakage ◽  
Glucose Reduction ◽  
Membrane Destabilization

In this study, spherical silver nanoparticle (AgNP) was produced by sustainable chemical method i.e. glucose reduction method and it was utilized to analyse the bactericidal effect against the pathogens of clinical importance - E. coli (ATCC 10536) and Enterobacter sp., KL46 by membrane destabilization and protein leakage analyses. Minimum inhibitory/bactericidal and antibiogram analyses reported that 20ng/ml was enough to inhibit/kill bacterial cells. Even 20ng/ml concentration of AgNPs was found to destabilize membrane and lead to protein leakage from bacterial cells. 

Synthesis of Ag/AgCl–mesoporous silica nanocomposites using a simple aqueous solution-based chemical method and a study of their antibacterial activity on E. coli

Particuology ◽  
2011 ◽  
Vol 9 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Bhanudas Naik ◽  
Vilas Desai ◽  
Meenal Kowshik ◽  
Vadakkethonippurathu Sivankutty Prasad ◽  
Gerard Franklyn Fernando ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Antibacterial Activity ◽  
Mesoporous Silica ◽  
Chemical Method ◽  
E Coli ◽  
Silica Nanocomposites

Indoxyl-β-D-glucuronide, a novel chromogenic reagent for the specific detection and enumeration of Escherichia coli in environmental samples

1988 ◽  
Vol 34 (5) ◽  
pp. 690-693 ◽  
Author(s):  
Arthur Newton Ley ◽  
Raymond John Bowers ◽  
Saul Wolfe
Keyword(s):  
Escherichia Coli ◽  
Large Scale ◽  
Membrane Filter ◽  
Chloroacetic Acid ◽  
Specific Detection ◽  
E Coli ◽  
Filter Test ◽  
Almost All ◽  
First Time ◽  
Specific Reagent

About 97% of Escherichia coli strains produce β-glucuronidase, but almost all other Enterobacteriaceae lack this enzyme. A D-glucopyranosiduronic acid (glucuronide) possessing a readily detectable β-linked aglycone should, therefore, constitute a specific reagent for the detection of this organism. For this purpose, the title compound has been synthesized for the first time. The synthesis proceeds in eight steps from readily available D-glucuronolactone, anthranilic acid, and chloroacetic acid and can be carried out on a large scale. The compound has the predicted properties: when included in the standard membrane filter test for the analysis of water, indoxyl-β-D-glucuronide allows specific detection of E. coli through the formation of blue colonies that are the result of rapid conversion of the liberated aglycone to indigo. The recovery of E. coli is easily measured and almost quantitative.

scholarly journals Intimin Gene (eae) Subtype-Based Real-Time PCR Strategy for Specific Detection of Shiga Toxin-Producing Escherichia coli Serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 in Cattle Feces

2013 ◽  
Vol 80 (3) ◽  
pp. 1177-1184 ◽  
Author(s):  
Delphine Bibbal ◽  
Estelle Loukiadis ◽  
Monique Kérourédan ◽  
Carine Peytavin de Garam ◽  
Franck Ferré ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Specific Detection ◽  
Content Type ◽  
E Coli ◽  
Cattle Feces ◽  
Pcr Assays

ABSTRACTShiga toxin-producingEscherichia coli(STEC) strains belonging to serotypes O157:H7, O26:H11, O103:H2, O111:H8, and O145:H28 are known to be associated with particular subtypes of the intimin gene (eae), namely, γ1, β1, ε, θ, and γ1, respectively. This study aimed at evaluating the usefulness of their detection for the specific detection of these five main pathogenic STEC serotypes in cattle feces. Using real-time PCR assays, 58.7% of 150 fecal samples were found positive for at least one of the four targetedeaesubtypes. The simultaneous presence ofstx,eae, and one of the five O group markers was found in 58.0% of the samples, and the five targetedstxpluseaeplus O genetic combinations were detected 143 times. However, taking into consideration the association betweeneaesubtypes and O group markers, the resultingstxpluseaesubtype plus O combinations were detected only 46 times. The 46 isolation assays performed allowed recovery of 22E. colistrains belonging to one of the five targeted STEC serogroups. In contrast, only 2 of 39 isolation assays performed on samples that were positive forstx,eaeand an O group marker, but that were negative for the correspondingeaesubtype, were successful. Characterization of the 24E. coliisolates showed that 6 were STEC, including 1 O157:H7, 3 O26:H11, and 2 O145:H28. The remaining 18 strains corresponded to atypical enteropathogenicE. coli(aEPEC). Finally, the more discriminatingeaesubtype-based PCR strategy described here may be helpful for the specific screening of the five major STEC in cattle feces.

Monoclonal Antibodies to Lipopolysaccharide O Antigens of Enterohemorrhagic Escherichia coli Strains in Serogroups O26, O45, O103, O111, O121, and O145

2015 ◽  
Vol 78 (7) ◽  
pp. 1252-1258 ◽  
Author(s):  
BRIAN W. BROOKS ◽  
CHERYL L. LUTZE-WALLACE ◽  
BURTON BLAIS ◽  
MARTINE GAUTHIER ◽  
MYLÈNE DESCHÊNES
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibodies ◽  
Enterohemorrhagic Escherichia Coli ◽  
Human Pathogens ◽  
Specific Detection ◽  
Coli Isolate ◽  
E Coli ◽  
O Antigens

Non-O157 enterohemorrhagic Escherichia coli in priority serogroups O26, O45, O103, O111, O121, and O145 are increasingly recognized as important human pathogens. In the present study, a panel of monoclonal antibodies (MAbs) to the lipopolysaccharide O antigens of E. coli in serogroups O26, O45, O103, O111, O121, and O145 was produced. The specificity was evaluated by examining the reactivity of the MAbs with 50 E. coli strains and 42 non–E. coli bacteria, and several MAbs highly specific for E. coli strains in each of the six non-O157 priority serogroups were identified. The use of these highly specific MAbs may be of considerable value for determining whether an E. coli isolate belongs to one of the six priority non-O157 serogroups, for developing specific detection assays for these organisms, and for characterizing the lipopolysaccharide O antigens of isolates in these serogroups.

scholarly journals Fabrication of Paper Sheets Coatings Based on Chitosan/Bacterial Nanocellulose/ZnO With Enhanced Antibacterial and Mechanical Properties

2021 ◽  
Author(s):  
Joanna Jabłońska ◽  
Magdalena Onyszko ◽  
Maciej Konopacki ◽  
Adrian Augustyniak ◽  
Rafał Rakoczy ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Bacterial Cellulose ◽  
Food Packaging ◽  
Cellulose Nanofibers ◽  
Mechanical Activity ◽  
Bacterial Nanocellulose ◽  
E Coli ◽  
Cellulose Nanofibres ◽  
Tearing Resistance ◽  
Shape And Size

Here, we designed the composition of the coating of the paper sheets composed of chitosan, bacterial cellulose (nanofibres), and ZnO with boosted antibacterial and mechanical activity. We investigated the compositions with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles. The proposed processing of bacterial cellulose resulted in the formation of nanofibers. Antimicrobial behavior was tested using E. coli ATCC® 25922™ following ASTM E2149-13a standard. Mechanical properties of the paper sheets were measured by comparison of tearing resistance, tensile strength, and bursting strength according to ISO 5270 standard. The increased antibacterial response is assigned to the combination of chitosan and ZnO (independently of its shape and size), while the boosted mechanical behavior is due to bacterial cellulose nanofibers. Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.

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