scholarly journals Temperature-sensitive protein expression in protocells

2019 ◽  
Vol 55 (45) ◽  
pp. 6421-6424 ◽  
Author(s):  
Haiyang Jia ◽  
Michael Heymann ◽  
Tobias Härtel ◽  
Lei Kai ◽  
Petra Schwille
Keyword(s):  
Protein Expression ◽  
Temperature Regulation ◽  
Temperature Sensitive ◽  
Synthetic Temperature ◽  
Sense And Respond

We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers.

scholarly journals Rtr1 Is the Saccharomyces cerevisiae Homolog of a Novel Family of RNA Polymerase II-Binding Proteins

2008 ◽  
Vol 7 (6) ◽  
pp. 938-948 ◽  
Author(s):  
Patrick A. Gibney ◽  
Thomas Fries ◽  
Susanne M. Bailer ◽  
Kevin A. Morano
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Temperature Sensitive ◽  
Reading Frame ◽  
Acid Sensitivity ◽  
Number Of Genes ◽  
Sense And Respond ◽  
Transcriptional Complex ◽  
Yeast Homolog

ABSTRACT Cells must rapidly sense and respond to a wide variety of potentially cytotoxic external stressors to survive in a constantly changing environment. In a search for novel genes required for stress tolerance in Saccharomyces cerevisiae, we identified the uncharacterized open reading frame YER139C as a gene required for growth at 37°C in the presence of the heat shock mimetic formamide. YER139C encodes the closest yeast homolog of the human RPAP2 protein, recently identified as a novel RNA polymerase II (RNAPII)-associated factor. Multiple lines of evidence support a role for this gene family in transcription, prompting us to rename YER139C RTR1 (regulator of transcription). The core RNAPII subunits RPB5, RPB7, and RPB9 were isolated as potent high-copy-number suppressors of the rtr1Δ temperature-sensitive growth phenotype, and deletion of the nonessential subunits RPB4 and RPB9 hypersensitized cells to RTR1 overexpression. Disruption of RTR1 resulted in mycophenolic acid sensitivity and synthetic genetic interactions with a number of genes involved in multiple phases of transcription. Consistently, rtr1Δ cells are defective in inducible transcription from the GAL1 promoter. Rtr1 constitutively shuttles between the cytoplasm and nucleus, where it physically associates with an active RNAPII transcriptional complex. Taken together, our data reveal a role for members of the RTR1/RPAP2 family as regulators of core RNAPII function.

Temperature regulation in the tortoise Testudo hermanni, studied with indwelling probes

1996 ◽  
Vol 17 (2) ◽  
pp. 91-102 ◽  
Author(s):  
C. Huot-Daubremont ◽  
D. Bradshaw ◽  
C. Grenot
Keyword(s):  
Temperature Regulation ◽  
Temperature Sensitive ◽  
Behavioural Thermoregulation ◽  
Body Temperatures ◽  
South Eastern ◽  
Testudo Hermanni ◽  
Free Ranging

AbstractBehavioural thermoregulation has been studied in the terrestrial tortoise Testudo hermanni in south-eastern France by implanting temperature-sensitive probes in free-ranging individuals. Three tortoises were monitored over a period of nine months (July to March); they maintained body temperatures close to their preferendum for periods of several hours each day. These results are of interest as doubt has been expressed in the literature on the thermoregulatory capacities of Testudo hermanni in the northernmost part of its range.

scholarly journals Temperature Sensing by the dsrA Promoter

2003 ◽  
Vol 185 (22) ◽  
pp. 6609-6614 ◽  
Author(s):  
F. Repoila ◽  
S. Gottesman
Keyword(s):  
Temperature Control ◽  
Temperature Regulation ◽  
Point Mutations ◽  
Thermal Response ◽  
Interaction Model ◽  
Dna Interaction ◽  
Essential Elements ◽  
Temperature Sensitive ◽  
Sufficient Information

ABSTRACT Synthesis of the small regulatory RNA DsrA is under temperature control. The minimal dsrA promoter of 36 bp contains sufficient information to ensure such regulation. In vivo, we have analyzed the critical elements responsible for the temperature control of dsrA by using a collection of chimeric promoters combining various elements of the dsrA promoter and the lacUV5 promoter, which does not respond to temperature. Our results favor an RNA polymerase-DNA interaction model instead of a trans-acting factor for temperature regulation. While all of the elements of the dsrA promoter contribute to temperature-sensitive expression, the sequence of the −10 box and the spacer region are the essential elements for the thermal response of the dsrA promoter. The proper context for these promoter elements, including at least one of the flanking elements, the −35 region or the start site region, is also required. Point mutations demonstrate that the sequence of the −10 box imposes constraints on the length and the sequence of the spacer and/or its AT richness, even at low temperature. These results show a complex interdependence of different regions in the promoter for temperature regulation.

scholarly journals Genetic interactions at the FLA10 locus: suppressors and synthetic phenotypes that affect the cell cycle and flagellar function in Chlamydomonas reinhardtii.

Genetics ◽  
1991 ◽  
Vol 128 (3) ◽  
pp. 549-561 ◽  
Author(s):  
F G Lux ◽  
S K Dutcher
Keyword(s):  
Cell Cycle ◽  
Chlamydomonas Reinhardtii ◽  
Flagellar Apparatus ◽  
Genetic Interactions ◽  
Temperature Sensitive ◽  
Flagellar Motility ◽  
Synthetic Temperature ◽  
Flagellar Assembly ◽  
Cold Sensitive ◽  
Allele Specificity

Abstract Through the isolation of suppressors of temperature-sensitive flagellar assembly mutations at the FLA10 locus of Chlamydomonas reinhardtii, we have identified six other genes involved in flagellar assembly. Mutations at these suppressor loci, termed SUF1-SUF6, display allele specificity with respect to which fla10- mutant alleles they suppress. An additional mutation, apm1-122, which confers resistance to the plant herbicides amiprophos-methyl and oryzalin, was also found to interact with mutations at the FLA10 locus. The apm1-122 mutation in combination with three fla10- mutant alleles results in synthetic cold-sensitive cell division defects, and in combination with an additional pseudo-wild-type fla10- allele yields a synthetic temperature-sensitive flagellar motility phenotype. Based upon the genetic interactions of these loci, we propose that the FLA10 gene product interacts with multiple components of the flagellar apparatus and plays a role both in flagellar assembly and in the cell cycle.

Oncogenesis and the Lucké Virus

1968 ◽  
Vol 26 ◽  
pp. 200-201
Author(s):  
A. E. Vatter ◽  
J. Zambernard
Keyword(s):  
Rna Viruses ◽  
Vegetative State ◽  
Temperature Sensitive ◽  
Structural Level ◽  
Oncogenic Viruses ◽  
Dna Viruses ◽  
Leopard Frogs ◽  
Renal Adenocarcinoma ◽  
Induced Tumors ◽  
Northern Leopard Frogs

Oncogenic viruses, like viruses in general, can be divided into two classes, those that contain deoxyribonucleic acid (DNA) and those that contain ribonucleic acid (RNA). The RNA viruses have been recovered readily from the tumors which they cause whereas, the DNA-virus induced tumors have not yielded the virus. Since DNA viruses cannot be recovered, the bulk of present day investigations have been concerned with RNA viruses.The Lucké renal adenocarcinoma is a spontaneous tumor which occurs in northern leopard frogs (Rana pipiens) and has received increased attention in recent years because of its probable viral etiology. This hypothesis was first advanced by Lucké after he observed intranuclear inclusions in some of the tumor cells. Tumors with inclusions were examined at the fine structural level by Fawcett who showed that they contained immature and mature virus˗like particles.The use of this system in the study of oncogenic tumors offers several unique features, the virus has been shown to contain DNA and it can be recovered from the tumor, also, it is temperature sensitive. This latter feature is of importance because the virus can be transformed from a latent to a vegetative state by lowering or elevating the environmental temperature.

CFTR protein expression in healthy men spermatozoa is not correlated with fertilization rate of ova

2010 ◽  
Vol 34 (8) ◽  
pp. S12-S12
Author(s):  
Hong‑Ge Li ◽  
Chen Min Xu ◽  
Kun Li ◽  
Ya Ni ◽  
Wen‑Ying Chen ◽  
...  
Keyword(s):  
Protein Expression ◽  
Fertilization Rate ◽  
Healthy Men ◽  
Cftr Protein

Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients

2000 ◽  
Vol 111 (4) ◽  
pp. 1118-1121 ◽  
Author(s):  
A. Bellahcene ◽  
I. Van Riet ◽  
C. de Greef ◽  
N. Antoine ◽  
M. F. Young ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Protein Expression ◽  
Cell Lines ◽  
Myeloma Cell ◽  
Bone Sialoprotein ◽  
Multiple Myeloma Cell ◽  
Human Multiple Myeloma ◽  
Mrna And Protein Expression

660: Altered MUC-1 (EMA) Protein Expression Studied by Tissue Microarray Predicts Biochemical Failure after Radical Prostatectomy in Prostate Cancer

2007 ◽  
Vol 177 (4S) ◽  
pp. 222-222
Author(s):  
Mireia Musquera ◽  
Maria J. Ribal ◽  
Yolanda Arce ◽  
Humberto Villavicencio ◽  
Fernando Algaba ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Radical Prostatectomy ◽  
Protein Expression ◽  
Tissue Microarray ◽  
Biochemical Failure
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