Theragnosis by a miR-141-3p molecular beacon: simultaneous detection and sensitization of 5-fluorouracil resistant colorectal cancer cells through the activation of the TRIM13-associated apoptotic pathway

2019 ◽  
Vol 55 (52) ◽  
pp. 7466-7469 ◽  
Author(s):  
Sung Ung Moon ◽  
Yongkeun Park ◽  
Min Geun Park ◽  
Sung Kyu Song ◽  
Seok Hoo Jeong ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Molecular Beacon ◽  
Simultaneous Detection ◽  
Apoptotic Pathway ◽  
Colorectal Cancer Cells ◽  
Therapy And Diagnosis

Schematic of the ‘therapy and diagnosis at once (theragnosis)’ for 5-FU resistant colorectal cancer using the miR-141-3p molecular beacon.

scholarly journals Optical Biosensing System for the Detection of Survivin mRNA in Colorectal Cancer Cells Using a Graphene Oxide Carrier-Bound Oligonucleotide Molecular Beacon

Nanomaterials ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 510 ◽  
Author(s):  
Katarzyna Ratajczak ◽  
Bartlomiej Krazinski ◽  
Anna Kowalczyk ◽  
Beata Dworakowska ◽  
Slawomir Jakiela ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Graphene Oxide ◽  
Cancer Cells ◽  
Molecular Beacon ◽  
Survivin Mrna ◽  
Optical Biosensing ◽  
Colorectal Cancer Cells ◽  
Biosensing System

scholarly journals Synergistic Suppression Effect on Tumor Growth of Colorectal Cancer by Combining Radiotherapy With a TRAIL-Armed Oncolytic Adenovirus

2019 ◽  
Vol 18 ◽  
pp. 153303381985329 ◽  
Author(s):  
Hangxiang Gao ◽  
Xin Zhang ◽  
Ying Ding ◽  
Rong Qiu ◽  
Yupeng Hong ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Necrosis Factor ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Tumor Necrosis ◽  
Xenograft Model ◽  
Oncolytic Adenovirus ◽  
Apoptotic Pathway ◽  
Colorectal Cancer Cells ◽  
Necrosis Factor

The combination of gene therapy and radiation is a promising new treatment for cancer. This study aimed to clarify the synergistic effect of targeted oncolytic adenovirus (radiotherapy-tumor necrosis factor-related apoptosis-inducing ligand) and radiotherapy on colorectal cancer cells and elucidate the mechanisms of the underlying antitumor activity. Viability, cell cycle status, and apoptosis of treated colorectal cancer cells were determined via MTT and flow cytometric assays. The molecular mechanism underlying apoptotic pathway activation was elucidated through Western blot analysis of caspase-8, caspase-3, and PARP proteins. Combination treatment with radiotherapy-tumor necrosis factor-related apoptosis-inducing ligand and radiotherapy displayed significantly greater antitumor activity than either of the monotherapies. The primary mechanism behind the antitumor activity in the SW480 and Lovo colorectal cancer cell lines was apoptosis induction through the caspase pathway and G1 phase arrest. In an SW480 xenograft model of colorectal cancer, the combination therapy achieved a significantly greater reduction in tumor volume than the monotherapies. Overall, in this study, we demonstrate that the oncolytic radiotherapy-tumor necrosis factor-related apoptosis-inducing ligand construct can sensitize human colorectal cancer cells to radiation-induced apoptosis both in vitro and in vivo. Therefore, our findings point toward a novel synergistic approach to colorectal cancer treatment.

scholarly journals Single-molecule RNA sequencing for simultaneous detection of m6A and 5mC

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takahito Ohshiro ◽  
Masamitsu Konno ◽  
Ayumu Asai ◽  
Yuki Komoto ◽  
Akira Yamagata ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mass Spectrometry ◽  
Cancer Cells ◽  
Rna Sequencing ◽  
Single Molecule ◽  
Simultaneous Detection ◽  
Colorectal Cancer Cells ◽  
Base Modifications ◽  
Cell Propagation ◽  
Methylation Ratio

AbstractEpitranscriptomics is the study of RNA base modifications involving functionally relevant changes to the transcriptome. In recent years, epitranscriptomics has been an active area of research. However, a major issue has been the development of sequencing methods to map transcriptome-wide RNA base modifications. We have proposed a single-molecule quantum sequencer for mapping RNA base modifications in microRNAs (miRNAs), such as N6-methyladenosine (m6A) or 5-methylcytidine (5mC), which are related to cancer cell propagation and suppression. Here, we investigated 5mC and m6A in hsa-miR-200c-5p extracted from colorectal cancer cells and determined their methylation sites and rates; the data were comparable to those determined by mass spectrometry. Furthermore, we evaluated the methylation ratio of cytidine and adenosine at each site in the sequences and its relationship. These results suggest that the methylation ratio of cytidine and adenosine is facilitated by the presence of vicinal methylation. Our work provides a robust new tool for sequencing various types of RNA base modifications in their RNA context.

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