Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity

2019 ◽  
Vol 55 (35) ◽  
pp. 5064-5067 ◽  
Author(s):  
Qinghua Wang ◽  
Xiang Wang ◽  
Ying Sun ◽  
Xiaoxiao Yang ◽  
Leilei Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probes ◽  
Protease Activity ◽  
Ratiometric Imaging

Two novel FRET probes for selective real-time ratiometric imaging of SspA activity in live S. aureus cells.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

scholarly journals Real-Time Ratiometric Imaging of Micelles Assembly State in a Microfluidic Cancer-on-a-Chip

2020 ◽  
Author(s):  
Natalia Feiner-Gracia ◽  
Adrianna Glinkowska Mares ◽  
Marina Buzhor ◽  
Romen Rodriguez-Trujillo ◽  
Josep Samitier Marti ◽  
...  
Keyword(s):  
Real Time ◽  
Ratiometric Imaging

Ratiometric fluorescent probes with a self-immolative spacer for real-time detection of β-galactosidase and imaging in living cells

2018 ◽  
Vol 1033 ◽  
pp. 193-198 ◽  
Author(s):  
Xiangzhu Chen ◽  
Xiaodong Ma ◽  
Yuanyuan Zhang ◽  
Gui Gao ◽  
Jingjing Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Real Time Detection

scholarly journals Schnellnachweis von SARS-CoV-2 mit recombinase polymerase amplification

BIOspektrum ◽  
2020 ◽  
Vol 26 (6) ◽  
pp. 624-627
Author(s):  
Ole Behrmann ◽  
Iris Bachmann ◽  
Frank Hufert ◽  
Gregory Dame
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Dna Polymerase ◽  
Fluorescent Probes ◽  
Reverse Transcription ◽  
Nucleic Acid Detection ◽  
Detection Scheme ◽  
Recombination Proteins ◽  
Target Dna ◽  
Rapid Amplification

Abstract The COVID-19 pandemic highlights the need for fast and simple assays for nucleic acid detection. As an isothermal alternative to RT-qPCR, we outline the development of a detection scheme for SARS-CoV-2 RNA based on reverse transcription recombinase polymerase amplification (RT-RPA) technology. RPA uses recombination proteins in combination with a DNA polymerase for rapid amplification of target DNA at a constant temperature (39–42 °C) within 10 to 20 minutes and can be monitored in real-time with fluorescent probes.

scholarly journals Rhodamine scaffolds as real time chemosensors for selective detection of bisulfite in aqueous medium

2019 ◽  
Vol 18 (6) ◽  
pp. 1342-1349 ◽  
Author(s):  
Sumit Roy ◽  
Ashim Maity ◽  
Naren Mudi ◽  
Milan Shyamal ◽  
Ajay Misra
Keyword(s):  
Aqueous Medium ◽  
Real Time ◽  
Fluorescent Probes ◽  
Selective Detection ◽  
Turn On

Rhodamine and its derivatives have been widely used in designing fluorescent ‘turn on’ cation sensors, while very few rhodamine based fluorescent probes have been reported to date for the detection of anions in water.

Ribonuclease H-cleavable and recombinase-quenching fluorescent probes for the real-time detection of strand invasion based amplification

2019 ◽  
Vol 11 (43) ◽  
pp. 5568-5576
Author(s):  
Sonja Elf ◽  
Kevin E. Eboigbodin
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Real Time ◽  
Fluorescent Probes ◽  
Nucleic Acid Amplification ◽  
Ribonuclease H ◽  
Chain Reaction ◽  
Amplification Method ◽  
Polymerase Chain ◽  
Strand Invasion

SIBA is an established nucleic acid amplification method that is used as an alternative to polymerase chain reaction (PCR).

Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

2011 ◽  
Vol 47 (8) ◽  
pp. 2399-2401 ◽  
Author(s):  
Min Zhang ◽  
Bin-Cheng Yin ◽  
Xian-Fei Wang ◽  
Bang-Ce Ye
Keyword(s):  
Graphene Oxide ◽  
Real Time ◽  
Protease Activity ◽  
Real Time Monitoring
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